In Vitro Effects of Ethanol on Rabbit Platelet Aggregation, Secretion of Granule Contents, and Cyclic AMP Levels in the Presence of Prostacyclin

Margaret L Rand; Peter L Gross; Donna M Jakowec; Marian A Packham; J Fraser Mustard

doi:10.1055/s-0038-1646570

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1989; 61(02): 254-258
DOI: 10.1055/s-0038-1646570

Original Article

Schattauer GmbH Stuttgart

In Vitro Effects of Ethanol on Rabbit Platelet Aggregation, Secretion of Granule Contents, and Cyclic AMP Levels in the Presence of Prostacyclin

Margaret L Rand

The Department of Biochemistry, University of Toronto, Toronto, Ont., Canada, and the Department of Pathology, McMaster University, Hamilton, Ont., Canada

,

Peter L Gross

The Department of Biochemistry, University of Toronto, Toronto, Ont., Canada, and the Department of Pathology, McMaster University, Hamilton, Ont., Canada

,

Donna M Jakowec

The Department of Biochemistry, University of Toronto, Toronto, Ont., Canada, and the Department of Pathology, McMaster University, Hamilton, Ont., Canada

,

Marian A Packham

The Department of Biochemistry, University of Toronto, Toronto, Ont., Canada, and the Department of Pathology, McMaster University, Hamilton, Ont., Canada

,

J Fraser Mustard

The Department of Biochemistry, University of Toronto, Toronto, Ont., Canada, and the Department of Pathology, McMaster University, Hamilton, Ont., Canada

› Author Affiliations

Abstract

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Summary

Ethanol, at physiologically tolerable concentrations, inhibits platelet responses to low concentrations of collagen or thrombin, but does not inhibit responses of washed rabbit platelets stimulated with high concentrations of ADP, collagen, or thrombin. However, when platelet responses to high concentrations of collagen or thrombin had been partially inhibited by prostacyclin (PGI₂), ethanol had additional inhibitory effects on aggregation and secretion. These effects were also observed with aspirin- treated platelets stimulated with thrombin. Ethanol had no further inhibitory effect on aggregation of platelets stimulated with ADP, or the combination of ADP and epinephrine. Thus, the inhibitory effects of ethanol on platelet responses in the presence of PGI₂ were very similar to its inhibitory effects in the absence of PGI₂, when platelets were stimulated with lower concentrations of collagen or thrombin. Ethanol did not appear to exert its inhibitory effects by increasing cyclic AMP above basal levels and the additional inhibitory effects of ethanol in the presence of PGI₂ did not appear to be brought about by further increases in platelet cyclic AMP levels.

Keywords

Platelet responses - Ethanol - Prostacyclin - Cyclic AMP

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References

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