Subscribe to RSS
DOI: 10.1055/s-0038-1646608
Platelet Aggregation in Whole Blood - Studies with a Platelet Counting Technique - Methodological Aspects and Some Applications
Publication History
Received 07 September 1988
Accepted after revision 10 March 1989
Publication Date:
24 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198903/lookinside/thumbnails/10-1055-s-0038-1646608-1.jpg)
Summary
We describe a method for measuring platelet aggregation in whole blood by single platelet counting. The importance of a low stirring speed (100 rpm) to obtain agonist-specific aggregation is stressed. Despite this low stirring speed, the sensitivity to agonists equals that of the turbidometric technique in platelet-rich plasma. The optimal concentration of formaldehyde for fixing the aggregates, the effects of storage times and anticoagulant are studied. Applicability to the study of platelet function inhibitors or of inherited platelet function disorders is illustrated. It is concluded that this technique, used under the appropriate conditions, combines the advantage of measuring platelet aggregation in a more physiologtc environment with the advantages of the turbidometric technique such as high sensitivity.
-
References
- 1 Born GV R. Quantitative investigations into the aggregation of blood platelets. J Physiol (London) 1962; 162: 67-68
- 2 O’Brien JR. Platelet aggregation. II. Some results from a new method of study. J Clin Pathol 1962; 15: 452-455
- 3 Cardinal DC, Flower RJ. The electronic aggregometer: a novel device for assessing platelet behaviour in blood. J Pharmacol Methods 1980; 3: 135-158
- 4 Heynen MJ, Blockmans D, Verwilghen RL, Vermylen J. Congenital macrothrombocytopenia, leukocyte inclusions, deafness and proteinuria: functional and electronmicroscopic observations on platelets and megakaryocytes. Br J Haematol. (in press)
- 5 Lumley P, Humphrey P. A method for quantitating platelet aggregation and analyzing drug-receptor interactions on platelets in whole blood in vitro. J Pharmacol Methods 1981; 6: 153-166
- 6 Fox SC, Burgess-Wilson M, Heptinstall S, Mitchell J. Platelet aggregation in whole blood determined using the Ultra-Flo 100 Platelet Counter. Thromb Haemostas 1982; 48 (03) 327-329
- 7 Saniabadi A, Lowe G, Forbes C, Prentice C, Barbenel J. Platelet aggregation studies in whole human blood. Thromb Res 1983; 30: 625-632
- 8 Cheeseman J, Mills SP, Hardisty R. Platelet aggregometry on whole blood: the use of the ELT 8/ds blood cell counter in the investigation of bleeding disorders. Clin Lab Haematol 1984; 6: 265-272
- 9 Gresele P, Zoja C, Deckmyn H, Arnout J, Vermylen J, Verstraete M. Dipyridamole inhibits platelet aggregation in whole blood. Thromb Haemostas 1983; 50 (04) 1-5
- 10 Wu KK, Hoak JC. A new method for the detection of platelet aggregates in patients with arterial insufficiency. Lancet 1974; 2: 924-926
- 11 Heptinstall S, Fox S, Crawford J, Hawkins M. Inhibition of platelet aggregation in whole blood by dipyridamole and aspirin. Thromb Res 1986; 42: 215-223
- 12 Lad N, Lunt DO, Tuffin DP. The effect of thromboxane A2 synthesis inhibitors on platelet aggregation in whole blood. Thromb Res 1987; 46: 555-566
- 13 Saniabadi AR, Lowe GD O, Belch JJ F, Barbanel JC, Forbes CD. Effect of prostacyclin (epoprostenol) on the aggregation of human platelets in whole blood in vitro. Haemostasis 1984; 14: 487-494
- 14 Greer IA, Walker JJ, Calder AA, Forbes CD. Inhibition of platelet aggregation in whole blood by adrenoreceptor antagonists. Thromb Res 1985; 40: 631-643
- 15 Bevan J, Heptinstall S. Serotonin-induced platelet aggregation in whole blood and the effects of ketanserin and mepyramine. Thromb Res 1985; 38: 189-194
- 16 Born GV R, Cross MJ. The aggregation of blood platelets. J Physiol (London) 1963; 168: 178-195
- 17 Saniabadi AR, Lowe GD O, Barbenel JC, Forbes CD. A comparison of spontaneous platelet aggregation in whole blood with platelet rich plasma: additional evidence for the role of ADP. Thromb Haemostas 1984; 51 (01) 115-118
- 18 Saniabadi AR, Lowe GD O, Barbenel JC, Forbes CD. Further studies on the role of red blood cells in spontaneous platelet aggregation. Thromb Res 1985; 38: 225-232
- 19 Arnout J, Deckmyn H, Huybrechts E, Verstraete M. UDCG115 and 212, two benzimidazo-pyridazinones with broad spectrum antiplatelet activity. Thromb Haemostas 1985; 54 (01) 134 (Abstr 792)