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Thromb Haemost 1989; 61(03): 423-428
DOI: 10.1055/s-0038-1646608
Original Article
Schattauer GmbH Stuttgart

Platelet Aggregation in Whole Blood - Studies with a Platelet Counting Technique - Methodological Aspects and Some Applications

C Falcon
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
,
J Arnout
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
,
J Vermylen
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 07. September 1988

Accepted after revision 10. März 1989

Publikationsdatum:
24. Juli 2018 (online)

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Summary

We describe a method for measuring platelet aggregation in whole blood by single platelet counting. The importance of a low stirring speed (100 rpm) to obtain agonist-specific aggregation is stressed. Despite this low stirring speed, the sensitivity to agonists equals that of the turbidometric technique in platelet-rich plasma. The optimal concentration of formaldehyde for fixing the aggregates, the effects of storage times and anticoagulant are studied. Applicability to the study of platelet function inhibitors or of inherited platelet function disorders is illustrated. It is concluded that this technique, used under the appropriate conditions, combines the advantage of measuring platelet aggregation in a more physiologtc environment with the advantages of the turbidometric technique such as high sensitivity.

Keywords

Platelet aggregation, whole blood - Platelet aggregation, platelet counting
 

  • References

  • 1 Born GV R. Quantitative investigations into the aggregation of blood platelets. J Physiol (London) 1962; 162: 67-68
    PubMedSuche in Google Scholar
  • 2 O’Brien JR. Platelet aggregation. II. Some results from a new method of study. J Clin Pathol 1962; 15: 452-455
    CrossrefPubMedSuche in Google Scholar
  • 3 Cardinal DC, Flower RJ. The electronic aggregometer: a novel device for assessing platelet behaviour in blood. J Pharmacol Methods 1980; 3: 135-158
    CrossrefPubMedSuche in Google Scholar
  • 4 Heynen MJ, Blockmans D, Verwilghen RL, Vermylen J. Congenital macrothrombocytopenia, leukocyte inclusions, deafness and proteinuria: functional and electronmicroscopic observations on platelets and megakaryocytes. Br J Haematol. (in press)
    PubMed
  • 5 Lumley P, Humphrey P. A method for quantitating platelet aggregation and analyzing drug-receptor interactions on platelets in whole blood in vitro. J Pharmacol Methods 1981; 6: 153-166
    CrossrefPubMedSuche in Google Scholar
  • 6 Fox SC, Burgess-Wilson M, Heptinstall S, Mitchell J. Platelet aggregation in whole blood determined using the Ultra-Flo 100 Platelet Counter. Thromb Haemostas 1982; 48 (03) 327-329
    PubMedSuche in Google Scholar
  • 7 Saniabadi A, Lowe G, Forbes C, Prentice C, Barbenel J. Platelet aggregation studies in whole human blood. Thromb Res 1983; 30: 625-632
    CrossrefPubMedSuche in Google Scholar
  • 8 Cheeseman J, Mills SP, Hardisty R. Platelet aggregometry on whole blood: the use of the ELT 8/ds blood cell counter in the investigation of bleeding disorders. Clin Lab Haematol 1984; 6: 265-272
    PubMedSuche in Google Scholar
  • 9 Gresele P, Zoja C, Deckmyn H, Arnout J, Vermylen J, Verstraete M. Dipyridamole inhibits platelet aggregation in whole blood. Thromb Haemostas 1983; 50 (04) 1-5
    PubMedSuche in Google Scholar
  • 10 Wu KK, Hoak JC. A new method for the detection of platelet aggregates in patients with arterial insufficiency. Lancet 1974; 2: 924-926
    PubMedSuche in Google Scholar
  • 11 Heptinstall S, Fox S, Crawford J, Hawkins M. Inhibition of platelet aggregation in whole blood by dipyridamole and aspirin. Thromb Res 1986; 42: 215-223
    CrossrefPubMedSuche in Google Scholar
  • 12 Lad N, Lunt DO, Tuffin DP. The effect of thromboxane A2 synthesis inhibitors on platelet aggregation in whole blood. Thromb Res 1987; 46: 555-566
    CrossrefPubMedSuche in Google Scholar
  • 13 Saniabadi AR, Lowe GD O, Belch JJ F, Barbanel JC, Forbes CD. Effect of prostacyclin (epoprostenol) on the aggregation of human platelets in whole blood in vitro. Haemostasis 1984; 14: 487-494
    PubMedSuche in Google Scholar
  • 14 Greer IA, Walker JJ, Calder AA, Forbes CD. Inhibition of platelet aggregation in whole blood by adrenoreceptor antagonists. Thromb Res 1985; 40: 631-643
    CrossrefPubMedSuche in Google Scholar
  • 15 Bevan J, Heptinstall S. Serotonin-induced platelet aggregation in whole blood and the effects of ketanserin and mepyramine. Thromb Res 1985; 38: 189-194
    CrossrefPubMedSuche in Google Scholar
  • 16 Born GV R, Cross MJ. The aggregation of blood platelets. J Physiol (London) 1963; 168: 178-195
    CrossrefPubMedSuche in Google Scholar
  • 17 Saniabadi AR, Lowe GD O, Barbenel JC, Forbes CD. A comparison of spontaneous platelet aggregation in whole blood with platelet rich plasma: additional evidence for the role of ADP. Thromb Haemostas 1984; 51 (01) 115-118
    PubMedSuche in Google Scholar
  • 18 Saniabadi AR, Lowe GD O, Barbenel JC, Forbes CD. Further studies on the role of red blood cells in spontaneous platelet aggregation. Thromb Res 1985; 38: 225-232
    CrossrefPubMedSuche in Google Scholar
  • 19 Arnout J, Deckmyn H, Huybrechts E, Verstraete M. UDCG115 and 212, two benzimidazo-pyridazinones with broad spectrum antiplatelet activity. Thromb Haemostas 1985; 54 (01) 134 (Abstr 792)
    PubMedSuche in Google Scholar