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Thromb Haemost 1978; 39(01): 177-185
DOI: 10.1055/s-0038-1646667
Original Article
Schattauer GmbH Stuttgart

The Effect of Mitomycin C on Platelet Aggregation and Adenosine 3′, 5′-Monophosphate Metabolism

Shuichi Hashimoto
The Radioisotope Research Laboratory and Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo 108, Japan
,
Sachiko Shibata
The Radioisotope Research Laboratory and Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo 108, Japan
,
Bonro Kobayashi
The Radioisotope Research Laboratory and Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo 108, Japan
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Publication History

Received 06 March 1977

Accepted 15 July 1977

Publication Date:
12 July 2018 (online)

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Summary

The effect of Mitomycin C on aggregation, adenosine 3′, 5′-monophosphate (cyclic AMP) metabolism and reactions induced by thrombin was studied in rabbit platelets. Mitomycin C inhibited the platelet aggregation induced by adenosine diphosphate or thrombin. The level of radioactive cyclic AMP derived from 8-14C adenine or 8-14C adenosine increased after incubating intact platelets with Mitomycin G. Formation of radioactive adenosine triphosphate also increased though mitochondrial oxidation was not stimulated. Similar effect was observed also in rabbit liver. Mitomycin C failed to stimulate platelet adenyl cyclase but inhibited cyclic AMP phosphodiesterase in the absence of theophylline. In the platelets preincubated with Mitomycin C, thrombin-induced inhibition of adenyl cyclase, stimulation of membrane-bound cyclic AMP phosphodiesterase, and release of 250,000 dalton protein from platelet membranes were prevented. These results suggest that Mitomycin C will affect cellular membrane structure and function, and this extranuclear effect of Mitomycin C will lead to inhibition of aggregation in blood platelets.

 

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