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Thromb Haemost 1978; 39(02): 466-473
DOI: 10.1055/s-0038-1646706
Original Article
Schattauer GmbH Stuttgart

Quantitation of Radio-Labelled Vitamin K1 and Vitamin K1 Epoxide in Plasma by High Pressure Liquid Chromatography

Thorir D Bjornsson
The Divisions of Clinical Pharmacology and Cardiology, Department of Medicine, Stanford University Medical Center, Stanford, California, USA
,
Sarah E Swezey
The Divisions of Clinical Pharmacology and Cardiology, Department of Medicine, Stanford University Medical Center, Stanford, California, USA
,
Peter J Meffin
The Divisions of Clinical Pharmacology and Cardiology, Department of Medicine, Stanford University Medical Center, Stanford, California, USA
,
Terrence F Blaschke
The Divisions of Clinical Pharmacology and Cardiology, Department of Medicine, Stanford University Medical Center, Stanford, California, USA
› Author Affiliations
Further Information

Publication History

Received 21 July 1977

Accepted 20 October 1977

Publication Date:
12 July 2018 (online)

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Summary

A convenient, accurate and reproducible high pressure liquid chromatographic method for the quantitation of radio-labelled vitamin K1 and vitamin K1 epoxide in plasma is described. The method involves the determination of total ether extractable radioactivity, and a chromatographic separation to determine the relative quantities of radio-labelled vitamin K1 and vitamin K1 epoxide. The method is useful over a wide range of ratios of the two compounds, and has a coefficient of variation of approximately 5%.

 

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