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Thromb Haemost 1978; 39(02): 496-503
DOI: 10.1055/s-0038-1646709
Original Article
Schattauer GmbH Stuttgart

Ornithine Decarboxylase Activity in Cultured Endothelial Cells Stimulated by Serum, Thrombin and Serotonin

P A D’Amore
The Department of Biology and Surgery; Boston University, Boston, Massachusetts, U.S.A.
,
H B Hechtman
The Department of Surgery, Horvard Medical School, Boston, Massachusetts, U.S.A.
,
D Shepro
The Department of Biology and Surgery; Boston University, Boston, Massachusetts, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 01 June 1977

Accepted 10 November 1977

Publication Date:
12 July 2018 (online)

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Summary

Ornithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10-5 M) or Lilly 11 0140 (10-6M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.

 

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