Thromb Haemost 1978; 39(03): 555-563
DOI: 10.1055/s-0038-1646728
Original Article
Schattauer GmbH Stuttgart

A Rapid Chromatographic Method for Quantitation of High Molecular Weight Fibrinogen Derivatives in Plasma

Vincenzo Musumeci
The Laboratory of Blood Coagulation, Department of Internal Medicine, the Catholic University, Rome, Italy
,
Dolly Culasso
The Laboratory of Blood Coagulation, Department of Internal Medicine, the Catholic University, Rome, Italy
,
Paolo Boni
The Laboratory of Blood Coagulation, Department of Internal Medicine, the Catholic University, Rome, Italy
› Author Affiliations
Further Information

Publication History

Received 18 October 1977

Accepted 14 December 1977

Publication Date:
12 July 2018 (online)

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Summary

High molecular weight fibrinogen derivatives were precipitated from 3 ml of plasma by 0.88 M ammonium sulphate. The resuspended precipitate was analyzed by chromatography on a 2.5 × 30 cm glass bead Bio-Gel 5 M column by using a flow rate of 60 cm/hr and continuous effluent monitoring at O. D. 280 nm. Complete elution of fibrinogen occurred in 50 min. Elution profile was handled according to the chromatographic plate theory analysis. Results obtained by our method were not significantly different from those obtained by conventional chromatography.