Preparation and Evaluation of an Antiserum to the Carboxymethylated Aα Chain of Human Fibrinogen

Summary

Antiserum to the carboxymethylated Aα chain of human fibrinogen has been produced in rabbits and its reactions investigated in a double diffusion system. The antiserum reacts with the carboxymethylated Aα chains, but not with carboxymethylatedγ chains, nor with carboxymethylated Bβ chains, provided that the antiserum had been absorbed with carboxymethylated Bβ chains.

The antiserum also reacts with Aα chains in the whole fibrinogen molecule, either in plasma or in the purified form, and spur formation was observed when intact fibrinogen was compared with high solubility fibrinogen, which contained little or no intact Aα chain.

• References

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