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Thromb Haemost 1978; 39(03): 574-581
DOI: 10.1055/s-0038-1646730
Original Article
Schattauer GmbH Stuttgart

Determination of Soluble Fibrin Monomer Complexes by Adsorption on Immobilized Fibrinogen

Axel W Stemberger
The Max-Planck-Institut für Biochemie, Am Klopferspitz, D-8033 Martinsried, West Germany
,
Helmut Hӧrmann
The Max-Planck-Institut für Biochemie, Am Klopferspitz, D-8033 Martinsried, West Germany
› Author Affiliations
Further Information

Publication History

Received 07 October 1977

Accepted 11 October 1977

Publication Date:
12 July 2018 (online)

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Summary

Determination of fibrin in plasma, also containing fibrinogen, is possible by selective adsorption on affinity columns prepared from highly purified fibrinogen. Desorption is complete using 1.0 M potassium bromide, pH 5.3. In addition, the cold-insoluble globulin is desorbed. Therefore, the eluate is checked on microtiter plates by the staphylococcal clumping test which selectively detects fibrin or fibrinogen. The procedure works well with simple laboratory equipment. The diagnosis of fibrinogen-fibrin complexes is demonstrated in some pathological plasmas.

 

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