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Thromb Haemost 1978; 39(03): 574-581
DOI: 10.1055/s-0038-1646730
DOI: 10.1055/s-0038-1646730
Original Article
Determination of Soluble Fibrin Monomer Complexes by Adsorption on Immobilized Fibrinogen
Further Information
Publication History
Received 07 October 1977
Accepted 11 October 1977
Publication Date:
12 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/197801/lookinside/thumbnails/10-1055-s-0038-1646730-1.jpg)
Summary
Determination of fibrin in plasma, also containing fibrinogen, is possible by selective adsorption on affinity columns prepared from highly purified fibrinogen. Desorption is complete using 1.0 M potassium bromide, pH 5.3. In addition, the cold-insoluble globulin is desorbed. Therefore, the eluate is checked on microtiter plates by the staphylococcal clumping test which selectively detects fibrin or fibrinogen. The procedure works well with simple laboratory equipment. The diagnosis of fibrinogen-fibrin complexes is demonstrated in some pathological plasmas.
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