Download PDF
Thromb Haemost 1978; 39(03): 582-591
DOI: 10.1055/s-0038-1646731
Original Article
Schattauer GmbH Stuttgart

Tissue Thromboplastin Activity of Isolated Human Monocytes

H Prydz
Cell Pathology Division, Clinical Research Centre, Northwick Park, Harrow, Middlesex, England
,
A C Allison
Cell Pathology Division, Clinical Research Centre, Northwick Park, Harrow, Middlesex, England
› Author Affiliations
Further Information

Publication History

Received 05 October 1977

Accepted 03 December 1977

Publication Date:
12 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

Certain agents that induce spreading of or increased content or release of lysosomal enzymes from human monocytes also induce a dramatic increase in tissue thromboplastin content of the cells. The increase is inhibited by cycloheximide and actinomycin D and does not appear in granulocyte, lymphocyte or platelet populations treated in the same way. The effect of several other substances on the development of monocyte tissue thromboplastin activity is reported.

 

  • References

  • 1 BjØrklid E, Storm E. 1977; Purification and some properties of the protein component of tissue thromboplastin from human brain. Biochemical Journal 165: 89
    CrossrefPubMedSearch in Google Scholar
  • 2 BjØRKLID E, Giercksky KE, Prydz H. 1978 An immunoradiometric assay for tissue thromboplastin. British Journal of Haematology, in press
    PubMedSearch in Google Scholar
  • 3 BjØrklid E, Storm E, Prydz H. 1973; The protein component of human brain thromboplastin. Biochemical and Biophysical Research Communications 55: 969
    CrossrefPubMedSearch in Google Scholar
  • 4 BjØrklid E, Storm-Mathisen J, Storm E, Prydz H. 1977; Localization of tissue thromboplastin in the human brain. Thrombosis and Haemostasis 37: 91
    Thieme ConnectPubMedSearch in Google Scholar
  • 5 Bohn E, MüLLER-BERGHAUS G. 1976; The effect of leukocyte and platelet transfusion on the activation of intravascular coagulation by endotoxin in granulocytopenic and thrombocytopenic rabbits. American Journal of Pathology 84: 239
    PubMedSearch in Google Scholar
  • 6 BØyum A. 1968; Isolation of mononuclear cells and granulocytes from human blood. Scandinavian Journal of Clinical and Laboratory Investigation. 21 Suppl (Suppl. 97) 77
    PubMedSearch in Google Scholar
  • 7 Gordon S, Unkeless JC, Cohn ZA. 1974; Induction of macrophage plasminogen activator by endotoxin stimulation and phagocytosis. Evidence for a two stage process. Journal of Experimental Medicine 140: 995
    CrossrefPubMedSearch in Google Scholar
  • 8 Hvatum M, Prydz H. 1966; Studies on tissue thromboplastin. I Solubilization with sodium deoxycholate. Biochimica et biophysica acta 130: 92
    CrossrefPubMedSearch in Google Scholar
  • 9 Little C, Aurebekk B, Otnaess AB. 1975; Purification by affinity chromatography of phospholipase C from Bacillus cereus. FEBS Letters 52: 175
    CrossrefPubMedSearch in Google Scholar
  • 10 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265
    PubMedSearch in Google Scholar
  • 11 Maynard JR, Heckman AA, Pitlick FA, Nemerson Y. 1975; Association of Tissue Factor Activity with the surface of cultured cells. Journal of Clinical Investigation, 55 814: 824
    PubMedSearch in Google Scholar
  • 12 Maynard JR, Fintel BS, Pitlick FA, Nemerson Y. 1976; a Tissue factor in culture cells. Metabolic control. Laboratory Investigations 35: 542
    PubMedSearch in Google Scholar
  • 13 Maynard JR, Fintel BS, Pitlick FA, Nemerson Y. 1976; b Tissue factor in cultured cells. Pharmacologic effects. Laboratory Investigations 35: 550
    PubMedSearch in Google Scholar
  • 14 Mizel SB, Wilson L. 1972; Inhibition of the transport of hexoses in mammalian cells by cytochalasin B. Journal of Biological Chemistry 247: 4102
    PubMedSearch in Google Scholar
  • 15 Niemetz J. 1971; The role of protein synthesis in the generation of tissue factor activity by lymphocytes. Proceedings of the Society for Experimental Biology and Medicine 139: 1276
    PubMedSearch in Google Scholar
  • 16 Otnaess AB, Little C, Sletten K, Wallin R, Johnson S, Flengsrud R, Prydz H. 1977; Some characteristics of phospholipase C from Bacillus cereus. European Journal of Biochemistry 79: 459
    CrossrefPubMedSearch in Google Scholar
  • 17 Prydz H, Allison AC, Schorlemmer HU. 1977; A further link between complement activation and blood coagulation. Nature 270: 173
    CrossrefPubMedSearch in Google Scholar
  • 18 Rickles FR, Levin J, Hardin JA, Barr CF, Conrad ME. 1977; Tissue factor generation by human mononuclear cells: Effects of endotoxin and dissociation of tissue factor generation from mitogenic response. Journal of Laboratory and Clinical Medicine 89: 792
    PubMedSearch in Google Scholar
  • 19 Rivers RPA, Hathaway WE, Weston WL. 1975; The endotoxin-induced coagulant activity of human monocytes. British Journal of Haematology 30: 311
    CrossrefPubMedSearch in Google Scholar
  • 20 Segal AW, Peters TJ. 1977; Analytical subcellular fractionation of human granulocytes with special reference to the localization of enzymes involved in microbicidal mechanisms. Clinical Science and Molecular Medicine 52: 429
    PubMedSearch in Google Scholar
  • 21 Schorlemmer HU, Burger R, Hylton W, Allison AC. 1977; Induction of lysosomal enzyme release from cultured macrophages by dextran sulphate. Clinical Immunology and Immunopathology 7: 88
    CrossrefPubMedSearch in Google Scholar
  • 22 Unkeless JC, Gordon S, Reich E. 1974; Secretion of plasminogen activator by stimulated macrophages. Journal of Experimental Medicine 139: 834
    CrossrefPubMedSearch in Google Scholar
  • 23 Vasalli JD, Reich E. 1977; Macrophage plasminogen ativator: Induction by products of activated lymphoid cells. Journal of Experimental Medicine 145: 429
    CrossrefPubMedSearch in Google Scholar
  • 24 Werb Z. 1975. In Van Furth R. (Ed.) Mononuclear Phagocytes; Blackwell, Oxford: 331-345
  • 25 Woods MW, Landy M, Whitby JL, Burk D. 1961; Metabolic effects of endotoxins on mammalian cells. Bact. Rev 25: 447
    PubMedSearch in Google Scholar
  • 26 Yam LT, Li CY, Crosby WH. 1971; Cytochemical identification of monocytes and granulocytes. American Journal of Clinical Pathology 55: 283
    CrossrefPubMedSearch in Google Scholar