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Thromb Haemost 1978; 39(03): 725-732
DOI: 10.1055/s-0038-1646749
Original Article
Schattauer GmbH Stuttgart

The Role of Prostaglandins in the ADP-Induced Aggregation of Rabbit Platelets Shown by the Use of 15-Hydroxyprostaglandin Dehydrogenase

Robert B Wallis
The CIBA Laboratories, Wimblehurst Road, Horsham, West Sussex, RH12 4AB, England
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Publication History

Received 27 June 1977

Accepted 01 November 1977

Publication Date:
12 July 2018 (online)

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Summary

The initial shape change and subsequent aggregation of platelets in citrated rabbit platelet-rich plasma caused by ADP in vitro was inhibited by 15-hydroxyprostaglandin dehydrogenase. This inhibition was NAD-dependent and was also seen when shape change and aggregation were initiated by sodium arachidonate or by collagen. The aggregation of gel-filtered rabbit platelets by thrombin was not, however, affected by removal of 15-hydroxyprostaglandins.

Indomethacin was found to inhibit ADP-induced aggregation but at a concentration (250 μM) much higher than that required to inhibit collagen-induced aggregation. Moreover the platelet release reaction had not taken place 3 min after ADP stimulation. The direct role 15-hydroxyprostaglandin production in ADP-induced aggregation of rabbit platelets is proposed. The involvement of 15-hydroxyprostaglandins in platelet aggregation caused by other inducers is also discussed.

 

  • References

  • 1 Aster RH, Jandl JH. 1964; Platelet sequestration in man. 1. Method. Journal of Clinical Investigation 43: 843
    CrossrefPubMedSearch in Google Scholar
  • 2 Bullock G, Christian RA. 1976; A novel approach for enzyme histochemical and autoradiographic studies on single cells. Histochemical Journal 8: 291
    CrossrefPubMedSearch in Google Scholar
  • 3 Calkins J, Lane KP, Losasso B, Thurber LE. 1974; Comparative study of platelet aggregation in various species. Journal of Medicine 5: 292
    PubMedSearch in Google Scholar
  • 4 Drummond AH, Gordon JL. 1974; Rapid, sensitive microassay for platelet 5-HT. Thrombosis et Diathesis Haemorrhagica 31: 366
    PubMedSearch in Google Scholar
  • 5 Flower RJ, Blackwell GJ. 1976; The importance of phospholipase A2 in prostaglandin biosynthesis. Biochemical Pharmacology 25: 285
    CrossrefPubMedSearch in Google Scholar
  • 6 Hamberg M, Svensson J, Samuelsson B. 1975. Thromboxanes : A new group of biologically active compounds derived from prostaglandin endoperoxides. Proceedings of the National Academy of Sciences; U.S.A.: 72 2994
    Search in Google Scholar
  • 7 Hamberg M, Svensson J, Wakabayishi T, Samuelsson B. 1974. Isolation and structure of two prostaglandin endoperoxides that cause platelet aggregation. Proceedings of the National Academy of Sciences; U.S.A: 71 345
    Search in Google Scholar
  • 8 Hansen HS. 1974; Inhibition by indomethacin and aspirin of 15-hydroxyprostaglandin dehydrogenase in vitro. Prostaglandins 8: 75
    PubMedSearch in Google Scholar
  • 9 Macintyre DE, Gordon JL. 1975; Calcium-dependent stimulation of platelet aggregation by PGE2 . Nature 258: 337
    CrossrefPubMedSearch in Google Scholar
  • 10 Maickel RP, Miller FP. 1966; Fluorescent products formed by reaction of indole derivatives and orthophthaldialdehyde. Analytical Chemistry 38: 1937
    CrossrefPubMedSearch in Google Scholar
  • 11 Matchinsky FM, Shanahan D, Ellerman J. 1974; An improved procedure for the enzymatic analysis of prostaglandins in the nanogram range. Analytical Biochemistry 60: 188
    CrossrefPubMedSearch in Google Scholar
  • 12 Mills DCB, Robb IA, Roberts GCK. 1968; The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation. Journal of Physiology 195: 715
    CrossrefPubMedSearch in Google Scholar
  • 13 Packham MA, Kinlough-Rathbone RL, Reimers HJ, Scott S, Mustard JF. 1976. Mechanisms of platelet aggregation independent of adenosine diphosphate. In: Silver MJ, Smith JB, Kocsis CC. (Eds) Prostaglandins in Haematology. Spectrum Publications Inc; Cocheton, New York: 1977, in press
    Search in Google Scholar
  • 14 Rao GHR, Gerrard JM, Eaton J, White JG. 1976; Detection of arachidonic acid (AA)-free radical formation, an essential step in platelet prostaglandin biosynthesis. Blood 48: 967
    PubMedSearch in Google Scholar
  • 15 Smith JB, Ingerman C, Kocsis JJ, Silver MJ. 1974; Formation of an intermediate in prostaglandin biosynthesis and its association with the platelet release reaction. Journal of Clinical Investigation 53: 1468
    CrossrefPubMedSearch in Google Scholar
  • 16 Sun FF, Armour SB, Bockstanz VR, Mcguire JC. 1976. Studies on 15-hydroxyprostaglandin dehydrogenase from monkey lung. In: Samuelsson B, Paoletti R. (eds.) Advances in prostaglandin and thromboxane research. vol. 1 Raven Press; New York: 163
    Search in Google Scholar
  • 17 Tangen O, Berman HJ, Marfey P. 1971; Gel filtration. A new technique for separation of blood platelets from plasma. Thrombosis et Diathesis Haemorrhagica 25: 268
    PubMedSearch in Google Scholar
  • 18 Taylor DG, Crawford N. 1974; An automated procedure for the extraction and fluorimetric determination of 5-hydroxytryptamine in platelet subcellular fractions. Analytical Biochemistry 59: 1
    CrossrefPubMedSearch in Google Scholar
  • 19 Westwick J. 1976; The effect of pulmonary metabolites of prostaglandins E1, E2 and F2αon ADP-induced aggregation of human and rabbit platelets. British Journal of Pharmacology 58: 297P
    PubMedSearch in Google Scholar