Direct Measurement of the Amount of Bound Collagen which Stimulates Platelet Aggregation

Summary

The amount of collagen which binds to platelets has been correlated with aggregating activity in a platelet aggregometer. Aggregating activity requires only 0.22 μg/ml (0.35 μg/ 10⁹ cells) of collagen bound, the reaction saturating with 4.9 μg/ml (7.9 μg/10⁹ cells). On the basis of the fraction of collagen bound and its hydroxyproline/proline ratio, it is concluded that the activity resides in the type I collagen component.

• References

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