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Thromb Haemost 1979; 41(03): 498-511
DOI: 10.1055/s-0038-1646802
Original Articles
Schattauer GmbH Stuttgart

Direct Measurement of the Amount of Bound Collagen which Stimulates Platelet Aggregation

Paul L Kronick
The Physical and Life Sciences Department, The Franklin Institute Research Laboratories, Philadelphia, Pa., 19103, USA
The Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pa., 19174, USA
,
Sergio A Jimenez
The Physical and Life Sciences Department, The Franklin Institute Research Laboratories, Philadelphia, Pa., 19103, USA
The Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pa., 19174, USA
› Author Affiliations
Further Information

Publication History

Received 14 December 1977

Accepted 20 June 1978

Publication Date:
09 July 2018 (online)

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Summary

The amount of collagen which binds to platelets has been correlated with aggregating activity in a platelet aggregometer. Aggregating activity requires only 0.22 μg/ml (0.35 μg/ 109 cells) of collagen bound, the reaction saturating with 4.9 μg/ml (7.9 μg/109 cells). On the basis of the fraction of collagen bound and its hydroxyproline/proline ratio, it is concluded that the activity resides in the type I collagen component.

 

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