Direct Measurement of the Amount of Bound Collagen which Stimulates Platelet Aggregation

Paul L Kronick; Sergio A Jimenez

doi:10.1055/s-0038-1646802

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1979; 41(03): 498-511
DOI: 10.1055/s-0038-1646802

Original Articles

Schattauer GmbH Stuttgart

Direct Measurement of the Amount of Bound Collagen which Stimulates Platelet Aggregation

Authors

Paul L Kronick

The Physical and Life Sciences Department, The Franklin Institute Research Laboratories, Philadelphia, Pa., 19103, USA

The Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pa., 19174, USA
Sergio A Jimenez

The Physical and Life Sciences Department, The Franklin Institute Research Laboratories, Philadelphia, Pa., 19103, USA

The Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pa., 19174, USA

Abstract

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Summary

The amount of collagen which binds to platelets has been correlated with aggregating activity in a platelet aggregometer. Aggregating activity requires only 0.22 μg/ml (0.35 μg/ 10⁹ cells) of collagen bound, the reaction saturating with 4.9 μg/ml (7.9 μg/10⁹ cells). On the basis of the fraction of collagen bound and its hydroxyproline/proline ratio, it is concluded that the activity resides in the type I collagen component.

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References

References
1 Balleisen L, Gay S, Marx R, Kühn K. 1975; Comparative investigation on the influence of human bovine collagen types I, II and III on the aggregation of human platelets. Klinische Wochenschrift 53: 903
2 Barnes MJ, Gordon JL, Macintyre DE. 1976; Platelet-aggregating activity of type I and type III collagens from human aorta and chicken skin. Biochemical Journal 160: 647
3 Bensusan HA, HOYT BL. 1958; Effect of various parameters on the rate of formation of fibers in collagen solutions. Journal of the American Chemical Society 80: 719
4 Born GV R. 1962; Quantitative investigations into the aggregation of blood platelets. Journal of Physiology (London) 162: 67 P
5 Brass LF, Bensusan HA. 1974; The role of collagen quaternary structure in the platelet- collagen interaction. Journal of Clinical Investigation 54: 1480
6 Cazenave JP, Assimeh SN, Painter RH, Packham MA, Mustard JF. 1976; Clq inhibition of the interaction of collagen with human platelets. The Journal of Immunology 116: 162
7 Chiang TM, Kang AH. 1976; Binding of chick skin collagen al chain by isolated membranes from human platelets. Journal of Biological Chemistry 251: 6347
8 Gay S, Balleisen L, Remberger K, Fietzek P, Adelmann BC, Kühn K. 1975; Immunohistological evidence for the presence of collagen type III in human arterial walls, arterial thrombi, and in leucocytes incubated with collagen. Klinische Wochenschrift 53: 899
9 Gibson JG, Rees SB, Mcmanus TJ, Scheitlin WA. 1957; A citrate-phosphate-dextrose solution for the preservation of human blood. American Journal of Clinical Pathology 28: 569
10 Gordon JL, Dingle JT. 1974; Binding of radiolabeled collagen to blood platelets. Journal of Cell Science 16: 157
11 Hovig T. 1963; Aggregation of rabbit blood platelets produced in vitro by saline “extract” of tendons. Thrombosis et Diathesis Haemorrhagica 9: 248
12 Hugues J, Hérion F, Nusgens B, LapiéRe CHM. 1976; Type III collagen and probably not type I collagen aggregates platelets. Thrombosis Research 9: 223
13 Jaffe RM, Deykin D. 1974; Evidence for a structural requirement for the aggregation of platelets by collagen. Journal of Clinical Investigation 53: 875
14 Jimenez SA, Dehm P, Prockop DJ. 1971; Further evidence for a transport form of collagen. Its extrusion and extracellular conversion to tropocollagen. Federation of European Biological Societies Letters 17: 245
15 Juva K, Prockop DJ. 1966; Modified procedure for the assay of³H- or¹⁴C-labelled hydroxyproline. Analytical Biochemistry 15: 77
16 Kivirikko KI, Laitinen Q, Prockop DJ. 1967; Modifications of a specific assay for hydroxyproline in urine. Analytical Biochemistry 19: 249
17 Kronick PL. 1974; Binding of collagen to platelets. Federation Proceedings 33: 1536
18 Kronick PL, Jimenez SA. 1975; a Quantitative determination of collagen-platelet binding. Thrombosis et Diathesis Haemorrhagica 34: 332
19 Kronick PL, Jimenez SA. 1975; b Collagen structure and binding to platelets. Federation Proceedings 34: 855
20 Kronick PL, Jimenez SA. 1976; Simultaneous aggregation and release from platelets monitored continuously in plasma. Thrombosis Research 9: 553
21 Muggli R, Baumgartner HR. 1973; Collagen induced platelet aggregation: requirement for tropocollagen multimers. Thrombosis Research 3: 715
22 Umbreit WW, Burris RH, Stauffer JF. 1964; Manometric Techniques. Burgess, Minneapolis, p 132
23 Wilner GD, Nossel HL, Procupez TL. 1974; Aggregation of platelets by collagen: polar active sites of insoluble human collagen. American Journal of Physiology 220: 1074