Direct Measurement of the Amount of Bound Collagen which Stimulates Platelet Aggregation

Paul L Kronick; Sergio A Jimenez

doi:10.1055/s-0038-1646802

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1979; 41(03): 498-511
DOI: 10.1055/s-0038-1646802

Original Articles

Schattauer GmbH Stuttgart

Direct Measurement of the Amount of Bound Collagen which Stimulates Platelet Aggregation

Paul L Kronick

The Physical and Life Sciences Department, The Franklin Institute Research Laboratories, Philadelphia, Pa., 19103, USA

The Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pa., 19174, USA

,

Sergio A Jimenez

The Physical and Life Sciences Department, The Franklin Institute Research Laboratories, Philadelphia, Pa., 19103, USA

The Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pa., 19174, USA

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Abstract

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Summary

The amount of collagen which binds to platelets has been correlated with aggregating activity in a platelet aggregometer. Aggregating activity requires only 0.22 μg/ml (0.35 μg/ 10⁹ cells) of collagen bound, the reaction saturating with 4.9 μg/ml (7.9 μg/10⁹ cells). On the basis of the fraction of collagen bound and its hydroxyproline/proline ratio, it is concluded that the activity resides in the type I collagen component.

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Referenzen

References
1 Balleisen L, Gay S, Marx R, Kühn K. 1975; Comparative investigation on the influence of human bovine collagen types I, II and III on the aggregation of human platelets. Klinische Wochenschrift 53: 903
2 Barnes MJ, Gordon JL, Macintyre DE. 1976; Platelet-aggregating activity of type I and type III collagens from human aorta and chicken skin. Biochemical Journal 160: 647
3 Bensusan HA, HOYT BL. 1958; Effect of various parameters on the rate of formation of fibers in collagen solutions. Journal of the American Chemical Society 80: 719
4 Born GV R. 1962; Quantitative investigations into the aggregation of blood platelets. Journal of Physiology (London) 162: 67 P
5 Brass LF, Bensusan HA. 1974; The role of collagen quaternary structure in the platelet- collagen interaction. Journal of Clinical Investigation 54: 1480
6 Cazenave JP, Assimeh SN, Painter RH, Packham MA, Mustard JF. 1976; Clq inhibition of the interaction of collagen with human platelets. The Journal of Immunology 116: 162
7 Chiang TM, Kang AH. 1976; Binding of chick skin collagen al chain by isolated membranes from human platelets. Journal of Biological Chemistry 251: 6347
8 Gay S, Balleisen L, Remberger K, Fietzek P, Adelmann BC, Kühn K. 1975; Immunohistological evidence for the presence of collagen type III in human arterial walls, arterial thrombi, and in leucocytes incubated with collagen. Klinische Wochenschrift 53: 899
9 Gibson JG, Rees SB, Mcmanus TJ, Scheitlin WA. 1957; A citrate-phosphate-dextrose solution for the preservation of human blood. American Journal of Clinical Pathology 28: 569
10 Gordon JL, Dingle JT. 1974; Binding of radiolabeled collagen to blood platelets. Journal of Cell Science 16: 157
11 Hovig T. 1963; Aggregation of rabbit blood platelets produced in vitro by saline “extract” of tendons. Thrombosis et Diathesis Haemorrhagica 9: 248
12 Hugues J, Hérion F, Nusgens B, LapiéRe CHM. 1976; Type III collagen and probably not type I collagen aggregates platelets. Thrombosis Research 9: 223
13 Jaffe RM, Deykin D. 1974; Evidence for a structural requirement for the aggregation of platelets by collagen. Journal of Clinical Investigation 53: 875
14 Jimenez SA, Dehm P, Prockop DJ. 1971; Further evidence for a transport form of collagen. Its extrusion and extracellular conversion to tropocollagen. Federation of European Biological Societies Letters 17: 245
15 Juva K, Prockop DJ. 1966; Modified procedure for the assay of³H- or¹⁴C-labelled hydroxyproline. Analytical Biochemistry 15: 77
16 Kivirikko KI, Laitinen Q, Prockop DJ. 1967; Modifications of a specific assay for hydroxyproline in urine. Analytical Biochemistry 19: 249
17 Kronick PL. 1974; Binding of collagen to platelets. Federation Proceedings 33: 1536
18 Kronick PL, Jimenez SA. 1975; a Quantitative determination of collagen-platelet binding. Thrombosis et Diathesis Haemorrhagica 34: 332
19 Kronick PL, Jimenez SA. 1975; b Collagen structure and binding to platelets. Federation Proceedings 34: 855
20 Kronick PL, Jimenez SA. 1976; Simultaneous aggregation and release from platelets monitored continuously in plasma. Thrombosis Research 9: 553
21 Muggli R, Baumgartner HR. 1973; Collagen induced platelet aggregation: requirement for tropocollagen multimers. Thrombosis Research 3: 715
22 Umbreit WW, Burris RH, Stauffer JF. 1964; Manometric Techniques. Burgess, Minneapolis, p 132
23 Wilner GD, Nossel HL, Procupez TL. 1974; Aggregation of platelets by collagen: polar active sites of insoluble human collagen. American Journal of Physiology 220: 1074