Somatostatin-Induced Changes in the Hemostasis of Rats

Summary

Somatostatin (SRIF) given intravenously, either as a single bolus or as a 2 hr infusion caused a significant prolongation of partial thromboplastin time (PTT) and depressed platelet counts and platelet aggregation in the rat. Following daily injections of protamine- zinc SRIF for 2 weeks the platelet count returned to normal, PTT remained prolonged and platelet aggregation was enhanced. The doses of SRIF used in this work were adequate to suppress the secretion of insulin and glucagon by the isolated pancreatic islets of treated animals.

• References

• 1 Bern MM. 1978; Platelet functions in diabetes mellitus. Diabetes 27: 342
• 2 Besser GM, Paxton AM, Johnson SA N, Moody EJ, Mortimer CH, Hall R, Gomez- Pan A, Schally AV, Kasun AJ, Coy DH. 1975; Impairment of platelet function by growth hormone release inhibiting hormone. Lancet 1: 1166
• 3 Brazeau P, Rivier J, Vale W, Guillemin R. 1974; Inhibition of growth hormone secretion in the rat by synthetic somatostatin. Endocrinology 94: 184
• 4 Brazeau P, Vale W, Brugis R, Ling N, Butcher M, Rivier J, Guillemin R. 1973; Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science 179: 77
• 5 Brecher G, Cronkite E. 1950; Morphology and enumeration of human platelets. Journal of Applied Physiology 3: 365
• 6 Broersma RJ, Bullemer GS, Mammen EF. 1969; Coagulation changes in shock and acidosis. Thrombosis et Diathesis Haemorrhagica Supp (Suppl. 01) 36: 171
• 7 Carmina E, Lanzura P, Janni A. 1976; Somatostatin and platelet function. New England Journal of Medicine 294: 226
• 8 Chideckel EW, Palmer J, Koerker DJ, Ensinck J, Davidson MB, Goodner CJ. 1975; Somatostatin blockade of acute and chronic stimuli of the endocrine pancreas and the consequences of this blockade on glucose hemostasis. Journal of Clinical Investigation 55: 754
• 9 Dobbs R, Sakuri H, Sasaki H, Faloona G, Valverde I, Beatens D, Orci L, Unger R. 1975; Glucagon: role in hyperglycemia of diabetes mellitus. Science 187: 544
• 10 Foá PP, Matsuyama T, Foá NL. 1977. Radioimmunoassay of glucagon. In: Abraham GE. ed Handbook of Radioimmunoassay. Marcel Dekker; New York: pp 299 318
• 11 Gerich JE, Loren M, Schneider J, Karam JH, Rivier J, Guillemin R, Forsham PH. 1974; Effects of somatostatin on plasma glucose and glucagon levels in human diabetes mellitus: pathophysiologic and therapeutic implications. New England Journal of Medicine 291: 544
• 12 Gordon YB, Martin MM, Mcneille AT, Chard T. 1973; Specific and sensitive determination of fibrinogen-degradation products by radioimmunoassay. Lancet 2: 1168
• 13 Hales CN, Randle PJ. 1963; Immunoassay of insulin with insulin antibody precipitate. Biochemical Journal 88: 137
• 14 Hansen AP, Lundbaek K. 1976; Somatostatin: a review of its effects especially in human beings. Diabete Mètab 2: 203
• 15 Koerker DJ, Harker LA, Goodner CJ. 1975; Effect of somatostatin on hemostasis in the baboon. New England Journal of Medicine 293: 476
• 16 Lacy PE, Kostianovsky M. 1967; Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16: 35
• 17 Langdell RD, Wagner RH, Brinkhous KM. 1953; Effects of antihemophilic factor on one-stage clotting tests. A presumptive test for hemophilia and a simple one-stage antihemophilic factor assay procedure. Journal of Laboratory are Clinical Medicine 41: 637
• 18 Micossi P, Pontiroli AE, Dunbar JC, Foá PP. 1978. Decrease in serum glucose and lipid levels and weight loss in normal and streptozotocin diabetic rats treated with protamine-zinc somatostatin. In: Crepaldi G, Lefèbvre PJ, and Alberti KG M M. (Eds.) Diabetes, Obesity and Hyperlipidemias. Academic Press; New York: pp 58 67
• 19 Mielke jr CH, Gerich JE, Lorenzi M, Tsalikian E, Rodvien R, Forsham PH. 1975; The effect of somatostatin on coagulation and platelet function in man. New England Journal of Medicine 293: 480
• 20 Nie NH, Hull CH, Jenkins JG, Steinbrenner K, Bent DH. 1975. Statistical Package for the Social Sciences, 2nd Edition. McGraw Hill; New York: pp 410 423
• 21 Quick AJ. 1940; The thromboplastin reagent for the determination of prothrombin. Science 92: 113
• 22 Sagel J, Colwell JA, Crook L, Laimins M. 1975; Increased platelet aggregation in early diabetes mellitus. Annals of Internal Medicine 82: 733
• 23 Sherwin RS, Fisher M, Hendler R, and Felig P. 1976; Hyperglucagonemia and blood glucose regulation in normal,obese and diabetic subjects. New England Journal of Medicine 294: 455
• 24 Vale W, Brazeau P, Rivier C, Brown M, Boss B, Rivier J, Burgus R, Ling N, Guillemin R. 1975; Somatostatin. Recent Progress in Hormone Research 31: 365