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Thromb Haemost 1979; 41(03): 608-621
DOI: 10.1055/s-0038-1646814
Original Articles
Schattauer GmbH Stuttgart

The Nucleated Thrombocytoid Cells. I. Electron Microscopic Studies on Chicken Blood Cells

H Janzarik
The Department of Medicine, Justus Liebig- Universität Giessen, and Department of Theoretical Medicine, Universität des Saarlandes, Homburg/Saar, W–Germany
,
E Morgenstern
The Department of Medicine, Justus Liebig- Universität Giessen, and Department of Theoretical Medicine, Universität des Saarlandes, Homburg/Saar, W–Germany
› Author Affiliations
Further Information

Publication History

Received 30 January 1977

Accepted 24 May 1977

Publication Date:
24 July 2018 (online)

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Summary

By analyses of electron micrographs it was investigated whether nucleated thrombocytes of chickens described in the literature can be differentiated from lymphoid cells with sufficient certainty as an independent cell system. The result was negative. The mixture of lymphoid cells and so-called thrombocytes investigated was extremely polymorphous and, unlike mammalian platelets, proved to be one cell system containing younger and more mature cells. In the literature cells with signs of overmaturity or activation are designated as thrombocytes. Our studies suggest that a genuine thrombocyte system of chickens and other birds is not existent. The cells hitherto considered as nucleated thrombocytes seem to belong to the lymphoid system.

 

  • References

  • 1 Archer RK. 1971. Blood coagulation. In: Bell DJ, Freeman BM. (eds.) Physiology and Biochemistry of the Domestic Fowl, Vol. 2. Academic Press; London: p 897
    Google Scholar
  • 2 Barr RD, Whang-Peng J, Perry S. 1975; Hemopoietic stem cells in human peripheral blood. Science 190: 284
    CrossrefPubMedGoogle Scholar
  • 3 Behnke O. 1970; Microtubules in disc-shaped blood cells. International Review of Experimental Pathology 9: 1
    PubMedGoogle Scholar
  • 4 Behnke O. 1974; Mikrotubuli und Mikrofilamente. Triangel, Sandoz — Zeitschrift fur Medizinische Wissenschaft 13: 7
    PubMedGoogle Scholar
  • 5 Belamarich FA, Fusari MH, Shepro D, Kien M. 1966; In vitro studies of aggregation of non-mammalian thrombocytes. Nature 212: 1579
    CrossrefPubMedGoogle Scholar
  • 6 Belamarich FA, Stiller RA, Shepro D. 1973; The release reaction: Is it a common mechanism in all types of hemostatic cells. Series Haematologica VI 3: 418
    PubMedGoogle Scholar
  • 7 Belamarich FA, Simoneit LW. 1973; Aggregation of duck thrombocytes by 5-Hydroxytrypt- amine. Microvascular Research 6: 229
    CrossrefPubMedGoogle Scholar
  • 8 Belamarich FA. 1975; The effect of Reserpine on aggregation of avian thrombocytes. Thrombosis et Diathesis Haemorrhagica 34: 360
    PubMedGoogle Scholar
  • 9 Bessis M. 1973. Living Blood Cells and their Ultrastructure. Springer-Verlag. Berlin-Heidelberg; New York:
    Google Scholar
  • 10 Blessing MH, cited by SCHULZ H. 1968. Thrombocyten und Thrombose im elektronenmikroskopis-chen Bild. Springer-Verlag, Berlin -Heidelberg; New York: p 46
    Google Scholar
  • 11 Blount WP. 1939; Thrombocyte formation in the domestic hen. The Veterinary Journal 95: 195
    PubMedGoogle Scholar
  • 12 Carlson HC, Sweeny PR, Tokaryk JM. 1968; Demonstration of phagocytic and trephocytic activities of chicken thrombocytes by microscopy and vital staining techniques. Avian Diseases 12: 700
    CrossrefPubMedGoogle Scholar
  • 13 Clawson CC, Cooper MD, Good RA. 1967; Lymphocyte fine structure in the bursa of Fabricius, the thymus, and the germinal centers. Laboratory Investigation 16: 407
    PubMedGoogle Scholar
  • 14 Dhingra LD, Parrish WB, Venzke WG. 1969; Electron microscopy of nongranular leucocytes and thrombocytes of chickens. American Journal of Veterinary Research 30: 1837
    PubMedGoogle Scholar
  • 15 Edmonds RH. 1969; In vivo uptake of particulate matter by the avian embryo thrombocyte. Journal of Cellular Biology 43: 33 a
    PubMedGoogle Scholar
  • 16 Enbergs H, Kriesten K. 1968; a Die weißen Blutzellen des Haushuhns im elektronen- mikroskopischen Bild. Deutsche Tierarztliche Wochenschrift 75: 271
    PubMedGoogle Scholar
  • 17 Enbergs H, Kriesten K. 1968; b Zytoplasmatische Feinstrukturen der Thrombozyten des Haushuhns. Experientia 24: 597
    CrossrefPubMedGoogle Scholar
  • 18 Han SS, Baker BL. 1964; The ultrastructure of megakaryocytes and blood platelets in the rat spleen. Anatomical Record 149: 251
    CrossrefPubMedGoogle Scholar
  • 19 Höfmann U. 1968; Die Riesenzellen in der Milz des Igels (Erinaceus europaeus L.). Zeitschrift fiir ’ Zellforschung und Mikroskopische Anatomie 91: 261
    PubMedGoogle Scholar
  • 20 Hoepke H. 1933; Beiträge zur Morphologie und Physiologie des Lymphgewebes. I. Die Milz winterschlafender Tiere. Zeitschrift für Anatomie und Entwicklungsgeschichte 98: 411
    PubMedGoogle Scholar
  • 21 Inouye A, Kataoka K, Sorimachi M. 1969; 5-Hydroxytryptamine releasing effect on domestic fowl thrombocytes of the alkali kidney extract and some drugs. European Journal of Pharmacology 8: 200
    CrossrefPubMedGoogle Scholar
  • 22 Janzarik H. 1976. Prinzipien der Haemostase bei Hühnem. In: Die Rolle der Blutplättchen bei der Thrombose. Das Problem der Hyperkoagulabilität. Lüscher EF. (ed.) F. K, Schattauer Verlag, Stuttgart; New York:
    Google Scholar
  • 23 Jordan HE. 1965. Comparative Hematology. In: Downey H. (ed.) Handbook of Hematology, Vol. 2. Paul B. Hoeber Inc; New York: p 700
    Google Scholar
  • 24 Klaschen LV. 1922; Untersuchungen über die Riesenzellen in der Mäusemilz. Virchows Archiv für Pathologische Anatomie 237: 184
    PubMedGoogle Scholar
  • 25 Kriesten K, Enbergs H. 1970; Zur Ultrastruktur der Lymphozyten des Haushuhns. Blut 20: 229
    CrossrefPubMedGoogle Scholar
  • 26 Kuruma I, Okada T, Kataoka K, Sorimachi M. 1970; Ultrastructural observation of 5- Hydroxytryptamine-storing granules in the domestic fowl thrombocytes. Zeitschrift fiir Zellforschung und Mikroskopische Anatomie 108: 268
    PubMedGoogle Scholar
  • 27 Lucas AM, Jamroz C. 1961. Atlas of Avian Hematology. Agriculture Monograph 25. United States Department of Agriculture; Washington:
    Google Scholar
  • 28 Maxwell MH, Trejo F. 1970; The ultrastructure of white blood cells and thrombocytes of the domestic fowl. British Veterinary Journal 126: 583
    CrossrefPubMedGoogle Scholar
  • 29 Maxwell MH. 1974; An ultrastructural comparison of the mononuclear leucocytes and thrombocytes in six species of domestic bird. Journal of Anatomy 117: 69
    PubMedGoogle Scholar
  • 30 Morgenstern E, Janzarik H. 1977; The microtubular cytoskeleton in avian thrombocytes and human blood platelets and its correlations to certain organelles. Mikroskopie (Wien) 33: 47
    PubMedGoogle Scholar
  • 31 Nirmalan GP, Atwal OS, Carlson HC. 1972; Ultrastructural studies on the leucocytes and thrombocytes in the circulating blood of Japanese quail. Poultry Science 51: 2050
    CrossrefPubMedGoogle Scholar
  • 32 Rowsell HC. 1968; Hemostasis in the chicken and turkey. Federation Proceedings 27: 627
    PubMedGoogle Scholar
  • 33 Schumacher A. 1965; Zur submikroskopischen Struktur der Thrombozyten, Lymphozyten und Monozyten des Haushuhnes (gallus domesticus). Zeitschrift fur Zellforschung und Mikroskopische Anatomie 66: 219
    CrossrefPubMedGoogle Scholar
  • 34 Sengar DP S, Douglas RJ, Jerome FN. 1968; Thrombocyte antigens in chickens. Poultry Science 47: 1632
    CrossrefPubMedGoogle Scholar
  • 35 Simoneit LW, Belamarich FA, Shepro D. 1970; The localization of 5-Hydroxytryptamine in the blood cells of non-mammalian vertebrates. Journal of Cellular Biology 47: 192 a
    PubMedGoogle Scholar
  • 36 Simpson CF. 1968; Ultrastructural features of the turkey thrombocyte and lymphocyte. Poultry Science 47: 848
    CrossrefPubMedGoogle Scholar
  • 37 Sorimachi M, Kataoka K, Inouye A. 1970; Release in vitro of 5-Hydroxytryptamine from spindle cells of the domestic fowl. European Journal of Pharmacology 10: 243
    CrossrefPubMedGoogle Scholar
  • 38 Sorimachi M, Kataoka K, Hori S, Hori Sh, Fujisawa H. 1973; Tryptophan hydrolase in nucleated thrombocytes of the domestic fowl. European Journal of Biochemistry 33: 486
    CrossrefPubMedGoogle Scholar
  • 39 Sorimachi M, Kataoka K, Inouye A. 1974; Uptake of 5-Hydroxytryptamine by domestic fowl thrombocytes (spindle cells) in vitro. Naunyn-Schmiedeberg’s Archiv fiir Pharmakologie 283: 53
    PubMedGoogle Scholar
  • 40 Stalsberg H, Prydz H. 1963; Studies on chick embryo thrombocytes. I. Morphology and development. II. Function in primary hemostasis. Thrombosis et Diathesis Haemorrhagica 9: 279
    PubMedGoogle Scholar
  • 41 Sterz I. 1972 Elektronenmikroskopische und virologische Untersuchungen an Hiihnerthrombozy- ten nach Infektion mit dem Virus der klassischen Gefliigelpest (KP) in vitro und in vivo. Dissertation Giessen.
    PubMedGoogle Scholar
  • 42 Sterz I, Weiss E. 1974; Herkunft und Phagocytosefahigkeit der Huhnerthrombozyten. Deutsche tierarztliche Wochenschrift 81: 311
    PubMedGoogle Scholar
  • 43 Stiller RA, Belamarich FA, Shepro D. 1975; Aggregation and release in thrombocytes of the duck. American Journal of Physiology 229: 206
    PubMedGoogle Scholar
  • 44 Stockinger L, Weisel M, Lechner K. 1969; Thrombozytenpräparation und Auswertung. Mikroskopie 25: 362
    PubMedGoogle Scholar
  • 45 Stokes EE, Firkin BG. Studies of the peripheral blood of the Port Jackson shark (Heterodontus portusjacksoni) with particular reference to the thrombocyte. British Journal of Haematology 20: 427
    PubMedGoogle Scholar
  • 46 Sweeny PR, Carlson HC. 1968; Electron microscopy and histochemical demonstration of lysosomal structures in chicken thrombocytes. Avian Diseases 12: 636
    CrossrefPubMedGoogle Scholar
  • 47 Yamada E. 1957; The fine structure of megakaryocytes in the mouse spleen. Acta Anatomica 29: 267
    CrossrefPubMedGoogle Scholar