Ionophoretic Activities of Phospholipids on Human Platelets

Yasuo Ikeda; Masao Kikuchi; Keisuke Toyama; Kiyoaki Watanabe; Yasuhiko Ando

doi:10.1055/s-0038-1646837

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1979; 41(04): 779-786
DOI: 10.1055/s-0038-1646837

Original Articles

Schattauer GmbH Stuttgart

Ionophoretic Activities of Phospholipids on Human Platelets

Yasuo Ikeda

The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan

,

Masao Kikuchi

The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan

,

Keisuke Toyama

The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan

,

Kiyoaki Watanabe

^*Department of Clinical Pathology, School of Medicine, Keio University, Tokyo, Japan

The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan

,

Yasuhiko Ando

^*Department of Clinical Pathology, School of Medicine, Keio University, Tokyo, Japan

The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan

› Author Affiliations

Abstract

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Summary

The effects of phospholipids on calcium uptake by human platelets were investigated utilizing ⁴⁵CaCl₂. Among the phospholipids tested, cardiolipin and phosphatidic acid exerted a significant increase of calcium uptake by platelets. Calcium uptake was dependent upon cardiolipin concentration and incubation time. These phospholipids enhanced platelet aggregation induced by ADP (2 μM) or epinephrine (0.5 μg/ml). Cardiolipin caused the significant release of serotonin from platelets in the absence of externally added calcium in the medium.

These results may suggest that phospholipids activate platelets through their ionophoretic activities.

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