PDF herunterladen
Thromb Haemost 1979; 41(04): 779-786
DOI: 10.1055/s-0038-1646837
Original Articles
Schattauer GmbH Stuttgart

Ionophoretic Activities of Phospholipids on Human Platelets

Yasuo Ikeda
The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan
,
Masao Kikuchi
The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan
,
Keisuke Toyama
The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan
,
Kiyoaki Watanabe
*   Department of Clinical Pathology, School of Medicine, Keio University, Tokyo, Japan
The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan
,
Yasuhiko Ando
*   Department of Clinical Pathology, School of Medicine, Keio University, Tokyo, Japan
The Department of Hematology, School of Medicine, Keio University, Tokyo, Japan
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 19. Dezember 1977

Accepted 05. Mai 1978

Publikationsdatum:
09. Juli 2018 (online)

Auch verfügbar auf
  als PDF herunterladen  Lizenzen und Reprints

Summary

The effects of phospholipids on calcium uptake by human platelets were investigated utilizing 45CaCl2. Among the phospholipids tested, cardiolipin and phosphatidic acid exerted a significant increase of calcium uptake by platelets. Calcium uptake was dependent upon cardiolipin concentration and incubation time. These phospholipids enhanced platelet aggregation induced by ADP (2 μM) or epinephrine (0.5 μg/ml). Cardiolipin caused the significant release of serotonin from platelets in the absence of externally added calcium in the medium.

These results may suggest that phospholipids activate platelets through their ionophoretic activities.

 

  • References

  • 1 Born GV R. 1962; Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 194: 927
    PubMedSuche in Google Scholar
  • 2 Charo IF, Feinman RD, Detwiler TC. 1976; Inhibition of platelet secretion by an antagonist of intracellular calcium. B. B. Research Communication 72: 1462
    PubMedSuche in Google Scholar
  • 3 Feinman RD, Detwiler TC. 1974; Platelet secretion induced by divalent cation ionophores. Nature (London) 249: 172
    CrossrefPubMedSuche in Google Scholar
  • 4 Hallman B, Sehlin J, TÄljedal IB. 1976; Calcium and secretion: Distinction between two pools of glucose-sensitive calcium in pancreatic islets. Science 194: 1421
    CrossrefPubMedSuche in Google Scholar
  • 5 Harris RA, Farmer B. 1973; Stimulation of ion transport by phosphoglycerides. Lipids 8: 717
    CrossrefPubMedSuche in Google Scholar
  • 6 Iked AY, Steiner M. 1976; Isolation of platelet microtubule protein by an immunosorptive method. Journal of Biological Chemistry 251: 6135
    PubMedSuche in Google Scholar
  • 7 Joist JH, Dolezel G, Lloyd JV, Mustard JF. 1976; Phospholipid transfer between plasma and platelets in vitro. Blood 48: 199
    PubMedSuche in Google Scholar
  • 8 Leung NL, Kinlough-Rathbone RL, Mustard JF. 1977; Thrombin-induced changes in phosphatidic acid (PA) and phosphoinositides. Thrombosis and Haemostasis 38: 7
    PubMedSuche in Google Scholar
  • 9 Manery JF. 1969. In: Mineral Metabolism. Cowar CL, Bronner F. (Ed.) Academic Press; New York: P 405
    Suche in Google Scholar
  • 10 Massini P, LÜscher EF. 1974; Some effects of ionophores for divalent cations on blood platelets. Biochimica et Biophysica Acta 372: 109
    CrossrefPubMedSuche in Google Scholar
  • 11 MÜrer EH, Stewart GL. 1975; Calcium ionophore A23187. Rausch MA, DAY HJ. Thrombos Diathes Hemorrh 34: 72
    PubMedSuche in Google Scholar
  • 12 Rasmussen H. 1970; Cell communication, calcium ion and cyclic adenosine monophosphate. Science 170: 1970
    PubMedSuche in Google Scholar
  • 13 Robblee LS, Shepro D. 1976; The effect of external calcium and lanthanum on platelet calcium content and on the release reaction. Biochimica et Biophysica Acta 436: 448
    CrossrefPubMedSuche in Google Scholar
  • 14 Tyson CA, Zande HV, Green DE. 1976; Phospholipids as ionophores. Journal of Biological Chemistry 251: 1326
    PubMedSuche in Google Scholar
  • 15 White JG, Rao GH R, Gerrad JM. 1974; Effects of the ionophore A 23187 on blood platelets. American Journal of Pathology 77: 135
    PubMedSuche in Google Scholar