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Thromb Haemost 1989; 62(02): 718-722
DOI: 10.1055/s-0038-1646890
Original Article
Schattauer GmbH Stuttgart

Coated Pits and Vesicles Transfer Plasma Components to Platelet Granules

O Behnke
The Department of Anatomy C, University of Copenhagen, The Panum Institute, Copenhagen, Denmark
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Further Information

Publication History

Received 03 October 1988

Accepted after revision 12 April 1989

Publication Date:
30 June 2018 (online)

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Summary

Electron microscopy of mammalian blood platelets after contrast enhancement with tannic acid after an initial fixation in glutaraldehyde and osmium reveals numerous coated pits (c.p.) and vesicles (c.v.), indicating a process of receptor-mediated endocytosis. The c.p. may be located at any site of the plasma membrane or the canalicular, surface connected membrane system. C.v. fuse with platelet granules without losing their coat. Evidence for a continuous transfer of ambient fluid to granules via c. p. and c.v. was obtained by the use of fluid-phase markers.

It is proposed that the endocytic process may play a role in blood platelet activation.

Keywords

Blood platelets - Endocytosis - Coated vesicles - Granules
 

  • References

  • 1 Goldstein JL, Anderson RG W, Brown MS. Coated pits, coated vesicles, and receptor-mediated endocytosis. Nature 1979; 279: 679-685
    CrossrefPubMedGoogle Scholar
  • 2 Goldstein JL, Brown MS, Anderson RG W, Russell DW, Schneider WJ. Receptor-mediated endocytosis: Concepts emerging from the LDL receptor system. Annu Rev Cell Biol 1985; 1: 1-39
    CrossrefPubMedGoogle Scholar
  • 3 Wileman T, Harding C, Stahl P. Receptor-mediated endocytosis. Biochem J 1985; 232: 1-14
    CrossrefPubMedGoogle Scholar
  • 4 Morgenstem E. Coated membranes in blood platelets. Eur J Cell Biol 1982; 26: 315-318
    PubMedGoogle Scholar
  • 5 Bevers EM, Rosing J, Zwaal RF A. Platelets and coagulation. In: Platelets in Biology and Pathology III. MacIntyre DE, Gordon JL. (eds) Elsevier; Amsterdam: 1987. (; 13). 127-160
    Google Scholar
  • 6 George JN, Saucerman S, Levine SP, Knieriem LK. Immunoglobulin G is a platelet alpha granule-secreted protein. J Clin Invest 1985; 76: 2020-2025
    CrossrefPubMedGoogle Scholar
  • 7 Holt JC, Niewiarowski S. Biochemistry of granule proteins. Sem Hematol 1985; 22: 151-163
    PubMedGoogle Scholar
  • 8 Niewiarowski S, Varma KG. Biochemistry and physiology of secreted platelet proteins. In: Hemostasis and Thrombosis - Basic Principles and Clinical Practice. Colman RW, Hirsh J, Salzman EW. (eds) J. B. Lippincott; Philadelphia/Toronto: 1982: 421-430
    Google Scholar
  • 9 Walz DA. Platelet-released proteins as molecular markers for the activation process. Sem Thromb Hemostas 1984; 10: 270-279
    Article in Thieme ConnectPubMedGoogle Scholar
  • 10 Handagama PJ, George JN, Shuman MA, McEver RP, Bainton DF. Incorporation of a circulating protein into megakaryocyte and platelet granules. Proc Natl Acad Sci 1987; 84: 861-865
    CrossrefPubMedGoogle Scholar
  • 11 Walsh PN. Platelet washing by albumin density separation (ADGS). Adv Exp Med Biol 1972; 34: 245-256
    PubMedGoogle Scholar
  • 12 Behnke O, Bray D. Surface movements during the spreading of blood platelets. Eur J Cell Biol 1988; 46: 207-216
    PubMedGoogle Scholar
  • 13 Gelderblom HR. Fine structure, antigenic composition and functional aspects of the AIDS virus (HIV). Zeiss Information 1987; 5: 46-50
    PubMedGoogle Scholar
  • 14 Behnke O, Ammitzbøll T, lessen H, Klokker M, Nilausen K, Tranum-Jensen J, Olsson L. Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry. Eur J Cell Biol 1986; 41: 326-338
    PubMedGoogle Scholar
  • 15 De Roe C, Courtoy PJ, Baudhuin P. A model of protein-colloidal gold interactions. J Histochem Cytochem 1987; 35: 1191-1198
    CrossrefPubMedGoogle Scholar
  • 16 Morgenstern E, Reimers HJ. The platelet contacts during aggregation. Blut 1984; 48: 81-90
    CrossrefPubMedGoogle Scholar
  • 17 Mellman I. Membrane recycling during endocytosis. In: Lysosomes in Biology and Pathology. (; 7). Dingle JT, Dean RT, Sly W. (eds) Elsevier; Amsterdam: 1984: 201-229
    Google Scholar
  • 18 Pearse BM F, Bretscher MS. Membrane recycling by coated vesicles. Annu Rev Biochem 1981; 50: 85-101
    CrossrefPubMedGoogle Scholar
  • 19 Wencel-Drake JD, Plow EF, Kunicki TJ, Woods VL, Keller DM, Ginsberg HH. Localization of internal pools of membrane glycoproteins involved in platelet adhesive responses. Am J Pathol 1986; 124: 324-334
    PubMedGoogle Scholar
  • 20 Niewiarowski S. Platelet membrane components and receptors. Haematologica 1987; 20: 3-14
    PubMedGoogle Scholar
  • 21 Phillips DR. Receptors for platelet agonists. In: Platelet Membrane Glycoproteins. George JN, Nurden AT, Philips DR. (eds) Plenum Press; New York & London: 1985: 145-169
    Google Scholar
  • 22 Orci L, Glick BS, Rothman JE. A new type of coated vesicular carrier that appears not to contain clathrin: Its possible role in protein transport within the Golgi stack. Cell 1986; 46: 171-184
    CrossrefPubMedGoogle Scholar
  • 23 Rodriguez P, Bello O, Apitz-Castro R. Glycoprotein biosynthesis by human platelets. Biochem Med Metabol Biol 1987; 37: 197-204
    CrossrefPubMedGoogle Scholar
  • 24 George JN, Saucerman S. Platelet IgG, IgA, IgM and albumin: Correlation of platelet and plasma concentrations in normal subjects and in patients with ITP or dysproteinemia. Blood 1988; 72: 362-365
    PubMedGoogle Scholar
  • 25 White JG. The transfer of thorium particles from plasma to platelets and platelet granules. Am J Pathol 1968; 53: 567-575
    PubMedGoogle Scholar