Characterization of Monoclonal Antibodies to Human Tissue-Type Plasminogen Activator: Catalytic Inhibition and One-Two Chain Discriminatory Reactivities

Torgny Stigbrand; Lars Frängsmyr; Nils Bergsdorf; Per Wallen

doi:10.1055/s-0038-1646894

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1989; 62(02): 742-747
DOI: 10.1055/s-0038-1646894

Original Article

Schattauer GmbH Stuttgart

Characterization of Monoclonal Antibodies to Human Tissue-Type Plasminogen Activator: Catalytic Inhibition and One-Two Chain Discriminatory Reactivities

Torgny Stigbrand

The Department of Physiological Chemistry, University of Umei, Umeå, Sweden

,

Lars Frängsmyr

The Department of Physiological Chemistry, University of Umei, Umeå, Sweden

,

Nils Bergsdorf

The Department of Physiological Chemistry, University of Umei, Umeå, Sweden

,

Per Wallen

The Department of Physiological Chemistry, University of Umei, Umeå, Sweden

› Author Affiliations

Abstract

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Summary

A new set of monoclonal antibodies was generated against the tissue plasminogen activator (t-PA). One of the antibodies, 1:3 C5, was found to be able to distinguish between the one- and two-chain form of t-PA and also exerted significant amidolytic inhibitory activity. Several of the antibodies, as judged from their binding properties in immunosorbent tests, were found to be suitable for immunoaffinity purification purposes, i.e. 1:3C5, 1:3 G5, and 1:2 B9. Three of the mabs, 1:2 B9, 1:3 G5 and 2:2 BIO, were selectively reactive with the A-chain of t-PA, whereas indirect evidences indicated 1:3 C5 to be reactive with a conformational epitope on the B-chain. Three of the antibodies were reactive with porcine t-PA. This new set of antibodies should prove useful for structure-function investigations of t-PA.

Keywords

Tissue plasminogen activator - Fibrinolysis - Monoclonal antibodies

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References

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