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Thromb Haemost 1988; 60(02): 133-136
DOI: 10.1055/s-0038-1647016
Original Article
Schattauer GmbH Stuttgart

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

R Schneppenheim
*   The Department of Pediatrics, Klinikum der Christian-Albrechts-Universität Kiel, Hamburg, FRG
,
H Plendl
**   The Department of Human Genetics, Klinikum der Christian-Albrechts-Universität Kiel, Hamburg, FRG
,
U Budde
***   The General Hospital Harburg, Hamburg, FRG
› Author Affiliations
Further Information

Publication History

Received 15 April 1987

Accepted after revision 11 March 1988

Publication Date:
28 June 2018 (online)

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Summary

A luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

Keywords

Von Willebrand factor (vWF) - Multimers - Immunoblotting - Peroxidase-linked antibodies - Luminography
 

  • References

  • 1 Ruggeri ZM, Zimmermann TS. Variant von Willebrand’s disease – Characterization of two subtypes by analysis of multimeric composition of factor VIII/von Willebrand factor in plasma and platelets. J Clin Invest 1980; 65: 1318-1325
    CrossrefPubMedGoogle Scholar
  • 2 Ruggeri ZM, Zimmermann TS. The complex multimeric composition of factor VIII von Willebrand factor. Blood 1981; 57: 1140-1143
    PubMedGoogle Scholar
  • 3 Schneppenheim R, Plendl H, Grote W. Detection of factor VIII-related antigen by means of IEF in urea-agarose gels and an enzyme linked antibody. Electrophoresis 84 – Proceedings of the fourth meeting of the International Electrophoresis Society, Gottingen 1984. Neühoff V. (ed) Verlag Chemie; Weinheim: 1984: 481-483
    Google Scholar
  • 4 Miller MA, Palascak JE, Thompson MR, Martelo OJ. A modified SDS agarose gel method for determining factor VIII von Willebrand factor multimers using commercially available reagents. Thromb Res 1983; 39: 777-780
    PubMedGoogle Scholar
  • 5 Bukh A, Ingerslev J, Stenbjerg S, Hundahl Moeller NP. The multimeric structure of plasma FVIII: Ag studied by electroelution and immunoperoxidase detection. Thromb Res 1986; 43: 579-584
    CrossrefPubMedGoogle Scholar
  • 6 Lombardi R, Gelfi C, Righetti PG, Lattuada A, Mannucci PM. Electroblot and immunoperoxidase staining for rapid screening of the abnormalities of the multimeric structure of von Willebrand factor in von Willebrand’s disease. Thromb Haemostas 1986; 55: 246-249
    PubMedGoogle Scholar
  • 7 Brosstad F, Kjoenniksen I, Roenning B, Stormorken H. Visualization of von Willebrand factor multimers by enzyme-conjugated secondary antibodies. Thromb Haemostas 1986; 55: 276-278
    PubMedGoogle Scholar
  • 8 Aihara M, Sawada Y, Ueno K, Morimoto S, Yoshida Y, de Serres M, Cooper HA, Wagner RH. Visualization of von Willebrand factor multimers by immunoenzymatic stain using avidin-biotin peroxidase complex. Thromb Haemostas 1986; 55: 263-267
    PubMedGoogle Scholar
  • 9 Wang HX, George J, Thorpe GH G, Stott RA, Kricka LJ, Whitehead TP. Enhanced luminescence enzyme immunoassay for factor VIII related antigen. J Clin Pathol 1985; 38: 317-379
    CrossrefPubMedGoogle Scholar
  • 10 Schneppenheim R, Rautenberg P. A luminescence Western Blot with enhanced sensitivity for antibodies to Human Immunodeficiency Virus (HIV). Eur J Clin Microbiol 1987; 6: 49-51
    CrossrefPubMedGoogle Scholar