Download PDF
Thromb Haemost 1988; 60(02): 232-235
DOI: 10.1055/s-0038-1647036
Original Article
Schattauer GmbH Stuttgart

In Vivo Effects of a Low Molecular Weight Heparin Fragment on Platelet Aggregation and Platelet Dependent Hemostasis in Dogs

B Ljungberg
The Department of Medicine, Division of Thrombosis and Coagulation and Department of Experimental Surgery, Karolinska Hospital, Stockholm, Sweden
,
H Johnsson
The Department of Medicine, Division of Thrombosis and Coagulation and Department of Experimental Surgery, Karolinska Hospital, Stockholm, Sweden
› Author Affiliations
Further Information

Publication History

Received 20 October 1987

Accepted after revision 03 June 1988

Publication Date:
28 June 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

Plasma defibrinogenated dogs were used to study the influence of conventional heparin and a low molecular weight heparin fragment (Fragmin®, mean MW 5,000 d) on platelet dependent hemostasis. The heparins were given intravenously in gravimetri- cafly equal doses. The bleeding from standardized skin flap wounds and platelet aggregation (ADP and thrombin) was studied. In comparison, higher doses of the fragment than of heparin were required to increase the bleeding. ADP-induced aggregation in defibrinogenated platelet rich plasma (after addition of normal dog plasma) was potentiated by both heparins. After injection of heparin or the fragment, ADP induced platelet aggregation without prior addition of normal plasma to the testtube.

In conclusion the heparin fragment affected bleeding to a less extent than conventional heparin. One explanation might be a weaker inhibition of thrombin-induced platelet aggregation.

Keywords

Heparin-fragment - Bleeding time - Platelet aggregation - Dog
 

  • References

  • 1 Hirsh J. Heparin induced bleeding.. Nouv Rev Fr Hematol 1984; 26: 261-266
    PubMedSearch in Google Scholar
  • 2 Carter CJ, Kelton JG, Hirsh J, Cerskus A, Santos AV, Gent M. The relationship between the hemorrhagic and antithrombotic properties of low molecular weight heparin in rabbits.. Blood 1982; 59: 1239-1245
    PubMedSearch in Google Scholar
  • 3 Esquivel CO, Bergqvist D, Bjorck CG, Nilsson B. Comparison between commercial heparin, low molecular weight heparin and pentosan polysulfate on haemostasis and platelets in vivo.. Thromb Res 1982; 28: 389-399
    CrossrefPubMedSearch in Google Scholar
  • 4 Barrowcliffe TW. Anticoagulant activities of high and low molecular weight heparin fractions.. Br J Haematol 1979; 41: 573-583
    CrossrefPubMedSearch in Google Scholar
  • 5 Heiden D, Mielke CH, Rodvien R. Impairment by heparin of primary haemostasis and platelet (14C) 5-hydroxytryptamine release.. Br J Haematol 1977; 36: 427-436
    CrossrefPubMedSearch in Google Scholar
  • 6 Barradas MA, Mikhailidis DP, Epemolu E, Jeremy JY, Fonseca V, Dandona P. Comparison of the platelet pro-aggregatory effect of conventional heparins and a low molecular weight heparin fraction (CY 222).. Br J Haematol 1987; 67: 451-457
    CrossrefPubMedSearch in Google Scholar
  • 7 Westwick J, Scully MF, Poll C, Kakkar VV. Comparison of the effects of low molecular weight heparin and unfractionated heparin on activation of human platelets in vitro.. Thromb Res 1986; 42: 435-447
    CrossrefPubMedSearch in Google Scholar
  • 8 Borm JJ J, Krediet R, Sturk Aten, Cate JW. Heparin versus low molecular weight heparin K 2165 in chronic hemodialysis patients: A randomized cross-over study.. Haemostasis 1986; 16 Suppl. 2 59-68
    PubMedSearch in Google Scholar
  • 9 Johnsson H, Olsson P. Interference on the haemostasis in dogs with induced coagulopathies by drugs affecting the platelet function.. Haemostasis 1976; 4: 27-37
    PubMedSearch in Google Scholar
  • 10 Vermylen C, de VrekerRA, Verstraete M. A rapid enzymatic method for assay of fibrinogen fibrin polymerization time.. Clin Chim Acta 1973; 8: 418-424
    PubMedSearch in Google Scholar
  • 11 Teien AN, Lie M. Evaluation of an amidolytic heparin assay method: Increased sensivity by adding purified antithrombin III.. Thromb Res 1977; 10: 399-410
    CrossrefPubMedSearch in Google Scholar
  • 12 Bom GV R, Cross MJ. The aggregation of blood platelets.. J Physiol 1963; 168: 178-195
    CrossrefPubMedSearch in Google Scholar
  • 13 Stocker K. Defibrinogenation with thrombin-like snake venom enzymes. In: Handbook of Experimental Pharmacology 1978; 46: 451-484
    PubMedSearch in Google Scholar
  • 14 Egberg N, Johnsson H. Platelet aggregation induced by ADP and thrombin in reptilase defibrinated dogs.. Thromb Res 1972; 1: 95-112
    CrossrefPubMedSearch in Google Scholar
  • 15 Solum NO, Stormorken H. Influence of fibrinogen on the aggregation of washed human platelets induced by adenosin diphosphate, thrombin, collagen and adrenaline.. Scand J Lab Invest 1965; 17 Suppl 84 170-180
    PubMedSearch in Google Scholar
  • 16 Weiss HJ, Rogers J. Fibrinogen and platelets in the primary arrest of bleeding. Studies in two patients with congenital afibrogenemia.. N Engl J Med 1971; 285: 369-370
    CrossrefPubMedSearch in Google Scholar
  • 17 Saba SR, Saba HI, Morelli GA. Effect of collagen and citrate on heparin-related antiaggregatory activity.. Thromb Haemostas 1987; 58: 18 (Abstr.)
    PubMedSearch in Google Scholar
  • 18 Dunn FW, Soria C, Thomaidis A, Soria J, Dupuy E, Belluci S, Tobelem G. Interactions of platelets with standard heparin and low molecular weight fractions.. Nouv Rev Fr Hematol 1984; 26: 249-253
    PubMedSearch in Google Scholar