Binding of Plasma von Wllebrand Factor by Arterial Microfibrils

Summary

We developed an ELISA test to measure the binding of plasma von Willebrand factor (vWF) to arterial microfibrils and compared this interaction to the binding of vWF to collagen under the same conditions. We found that vWF binds to microfibrils in a similar manner as it binds to collagen: the binding was independent of the presence of cations, and temperature of incubation and was displaced by 1 M NaCl. Using purified ¹²⁵I-vwF we showed that the binding was saturable and could be displaced by cold vWF in excess. Using immunoblotting we showed that vWF binds to a 97 kDa protein present in the microfibrils different from the 128 kDa thrombospondin-like structure (GP 128) which in microfibrils is known to interact with blood platelets. These results indicate that in the subendothelium, microfibrils bind plasma vWF and this reinforces the thrombogenic role of these structures.

• References

• 1 Stel HV, Sakariassen KS, de Groot PG, van Mourik JA, Sixma JJ. Von Willebrand factor in the vessel wall mediates platelet adherence. Blood 1985; 65: 85-90
• 2 Tschopp TB, Weiss HJ, Baumgartner HR. Decreased adhesion of platelets to subendothelium in von Willebrand disease. J Lab Clin Med 1974; 83: 296-300
• 3 Birembaut P, Legrand YJ, Bariety J, Bretton R, Fauvel F, Belair MF, Pignaud G, Caen JP. Histochemical and ultrastructural characterization of subendothelial glycoprotein microfibrils interacting with platelets. J Histochem Cytochem 1982; 33: 75-80
• 4 Fauvel F, Grant ME, Legrand YJ, Souchon H, Tobelem G, Jackson DS, Caen JP. Interaction of blood platelets with a microfibrillar extract from adult bovine aorta: requirement for von Willebrand factor. Proc Natl Acad Sci USA 1983; 80: 551-554
• 5 Sakai LY, Keene DR, Engvall E. Fibrillin, a new 350 kD glycoprotein, is a component of extracellular microfibrils. J Cell Biol 1986; 103: 2499-2509
• 6 Kewley MA, Steven FS, Williams G. Preparation of a specific antiserum towards the microfibrillar proteins of elastic tissue. Immunology 1977; 32: 483-489
• 7 Serafini-Fracassini A, Ventrella G, Field M, Hinnie J, Onyezili NI, Griffiths R. Characterization of a structural glycoprotein from bovine ligamentum nuchae exhibiting dual amine oxidase activity. Biochemistry 1981; 20: 5424-5428
• 8 Gibson MA, Hugues JL, Fanning JC, Cleary EG. The major antigen of elastin-associated microfibrils is a 31 KD glycoprotein. J Biol Chem 1986; 261: 11429-11435
• 9 Fauvel-Lafeve F, Legrand YJ. Immunochemical identification of a thrombospondin-like structure in an arterial microfibrillar extract. Thromb Res 1988; 50: 305-316
• 10 Fauvel-Lafeve F, Picard P, Godeau G, Legrand YJ. Characterization of an antibody directed against a 128 kDa glycoprotein involved in the thrombogenicity of the elastin associated microfibrils. Biochem J 1988; 255: 251-258
• 11 Fauvel F, Campos-Oriola R, Leger D, Pignaud G, Rosenbaum J, Legrand YJ. Aortic endothelial cells in culture secrete glycoproteins reacting with blood platelets. Biochem Biophys Res Commun 1984; 123: 114-120
• 12 Legrand YJ, Fauvel F, Gutman N, Muh JP, Tobelem G, Souchon H, Karniguian A, Caen JP. Microfibrils platelet interaction: requirement of Von Willebrand factor. Thromb Res 1980; 19: 737-739
• 13 Nyman D. Interaction of collagen with the factor VIII antigen activity von Willebrand factor complex. Thromb Res 1977; 11: 433-438
• 14 Legrand YJ, Rodriguez-Zeballos A, Kartalis G, Fauvel F, Caen JP. Adsorption of factor VIII antigen-activity complex by collagen. Thromb Res 1978; 13: 909-911
• 15 Santoro SA, Cowan JF. Adsorption of von Willebrand factor by fibrillar collagen - Implications concerning the adhesion of platelets to collagen. Collagen Rel Res 1982; 2: 31-43
• 16 Scott DM, Griffin B, Pepper DS, Barnes MJ. The binding of purified factor VUI/von Willebrand factor to collagen of differing type and form. Thromb Res 1981; 24: 467-472
• 17 Sixma JJ, Sakariassen KS, Stel HV, Houdijk WP M, in der Maur DW, Hamer RJ, de Groot PG, Van Mourik JA. Functional domains on von Willebrand factor. Recognition of discrete tryptic fragments by monoclonal antibodies that inhibit interaction of von Willebrand factor with platelets and with collagen. J Clin Invest 1984; 74: 736-744
• 18 Kalafatis M, Takahashi Y, Girma JP, Meyer D. Localization of a collagen-interactive domain of human von Willebrand factor between amino acid residues Gly 911 and Glu 1,365. Blood 1987; 70: 1577-1583
• 19 Hormia M, Lehto VP, Virtanen I. Factor VIII related antigen: a pericellular matrix component of cultured human endothelial cells. Exp Cell Res 1983; 149: 483-497
• 20 Wagner D, Urban-Pickering M, Marder VJ. Von Willebrand protein binds to extracellular matrices independently of collagen. Proc Natl Acad Sc USA 1984; 81: 471-475
• 21 Rand JH, Sussman II, Gordon RE, Chu SV, Solomon V. Localization of factor VIII related antigen in human vascular subendothelium. Blood 1980; 55: 752-756
• 22 Fauvel F, Legrand YJ, Caen JP. Platelet adhesion to type I collagen and alpha 1(1)3 trimers: involvement of the C terminal alpha l(I)CB6a peptide. Thromb Res 1978; 12: 273-285
• 23 Wu QY, Drouet L, Carrier JL, Rothschild C, Berard M, Rouault C, Caen JP, Meyer D. Differential distribution of Von Willebrand factor in endothelial cells. Comparison between normal pigs and pigs with von Willebrand disease. Arteriosclerosis 1987; 7: 47-54
• 24 Legrand YJ, Fauvel F, Arbeille B, Leger D, Mouhli H, Gutman N, Muh JP. Activation of platelets by microfibrils and collagen: a comparative study. Lab Invest 1986; 54: 566-573
• 25 Suzuki K, Hashimoto S. Effect of dextran sulfate on thrombin activity. J Clin Pathol 1979; 32: 439-444
• 26 Vuento M, Vaheri A. Purification of fibronectin from human plasma by affinity chromatography under non-denaturing conditions. Biochem J. 1979; 183: 331-337
• 27 Saundry RH, Harrison P, Savidge GF. Chromatography of vWF on dextran sulfate Sepharose. Thromb Res 1987; 48: 641-652
• 28 Enayat MS, Hill FG H. Analysis of the complexity of the multimeric structure of factor VIII related antigen/von Willebrand protein using a modified electrophoretic technique. J Clin Pathol 1983; 36: 915-919
• 29 Hunter WM, Greenwood FC. Preparation of iodine 131 labelled human growth hormone of high specific activity. Nature 1962; 194: 495-496
• 30 Laemmli UK. Cleavage of structural glycoproteins during the assembly of the head of the bacteriophage T4. Nature 1970; 227: 680-685
• 31 Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4354
• 32 Sakariassen KS, Bolhuis PA, Sixma JJ. Human blood platelet adhesion to artery subendothelium is mediated by factor VUI-von Willebrand factor bound to the subendothelium. Nature 1979; 279: 636-639
• 33 de Groot PG, Ottenhof-Rovers M, van Mourik JA, Sixma JJ. Evidence that the primary binding site of von Willebrand factor that mediates platelet adhesion on subendothelium is not collagen. J Clin Invest 1988; 82: 65-73