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Thromb Haemost 1988; 59(03): 392-395
DOI: 10.1055/s-0038-1647502
Original Article
Schattauer GmbH Stuttgart

Evidence for a Discrete Binding Protein of Plasminogen Activator Inhibitor in Plasma

Björn Wiman
The Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden
,
Tomas Lindahl
The Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden
,
Åsa Almqvist
The Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden
› Author Affiliations
Further Information

Publication History

Received 24 September 1987

Accepted after revision 18 January 1988

Publication Date:
29 June 2018 (online)

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Summary

Gel-filtration experiments of mixtures of functionally active and inactive forms of plasminogen activator inhibitor (PAI) with human plasma or bovine serum albumin have provided evidence for the existence of a discrete binding protein of PAI in plasma. Most likely it is a glycoprotein with a molecular weight of approximately 150,000. The data suggest that it forms a very stable complex with functionally active forms of PAI, but not with the inactive or “latent” PAI. However, the PAI activity seems not to be significantly altered by the interaction with the binding protein. Assuming that a stoichiometric complex is formed, titration experiments suggest that a pool of normal human plasma contains about 40–50 mg of PAI-binding protein liter.

Keywords

Plasminogen activator inhibitor (PAI) - Protein-protein interaction - PAI-binding protein
 

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