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Thromb Haemost 1988; 60(01): 030-034
DOI: 10.1055/s-0038-1647629
Original Article
Schattauer GmbH Stuttgart

Asialo von Willebrand Factor Enhances Platelet Adhesion to Vessel Subendothelium

Eva Bastida
The Servicio Hemoterapia y Hemostasia, Hospital Clinic i Provincial, Facultad de Medicina, Universidad de Barcelona, Barcelona, Spain
,
Juan Monteagudo
The Servicio Hemoterapia y Hemostasia, Hospital Clinic i Provincial, Facultad de Medicina, Universidad de Barcelona, Barcelona, Spain
,
Antonio Ordinas
The Servicio Hemoterapia y Hemostasia, Hospital Clinic i Provincial, Facultad de Medicina, Universidad de Barcelona, Barcelona, Spain
,
Luigi De Marco
*   The Centro Immunotransfusionale, Ospedale Civile, Pordenone, Italy
,
Ricardo Castillo
The Servicio Hemoterapia y Hemostasia, Hospital Clinic i Provincial, Facultad de Medicina, Universidad de Barcelona, Barcelona, Spain
› Institutsangaben
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Publikationsverlauf

Received 19. August 1987

Accepted after revision 28. März 1988

Publikationsdatum:
24. Juli 2018 (online)

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Summary

Native von Willebrand factor (N-vWF) binds to platelets activated by thrombin, ADP or ristocetin. Asialo vWF (As-vWF) induces platelet aggregation in absence of platelet activators. N-vWF mediates platelet adhesion to vessel subendothelium at high shear rates. We have investigated the role of As-vWF in supporting platelet deposition to rabbit vessel subendothelium at a shear rate of 2,000 sec-1, using the Baumgartner perfusion system. We have studied the effects of the addition of As-vWF (from 2 to 12 μg/ml) to perfusates consisting of washed red blood cells, 4% human albumin and washed platelets. Our results show a significant increase in platelet deposition on subendothelium (p <0.01) in perfusions to which As-vWF had been added. Blockage of the platelet glycoproteins Ib and IIb/IIIa (GPIb and GPIIb/IIIa) by specific monoclonal antibodies (LJIb1 and LJCP8, respectively) resulted in a decrease of platelet deposition in both types of perfusates prepared with N-vWF and As-vWF. Our results indicate that As-vWF enhances platelet deposition to vessel subendothelium under flow conditions. Furthermore, they suggest that this effect is mediated by the binding of As-vWF to platelet membrane receptors, which in turn, promote platelet spreading and adhesion to the subendothelium.

Key words

Asialo von Willebrand factor - Platelet adhesion - Flow system
 

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