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Thromb Haemost 1988; 60(01): 054-058
DOI: 10.1055/s-0038-1647634
Original Article
Schattauer GmbH Stuttgart

Effect of Lupus Anticoagulant on Antithrombogenic Properties of Endothelial Cells - Inhibition of Thrombomodulin-Dependent Protein C Activation

Roger Cariou
1   The INSERM U 150, CNRS UA 334 - Hôpital Lariboisière, Paris, France
2   The CHU de Nantes, Hôtel Dieu, Service Hématologie Clinique, Nantes, France
,
Gérard Tobelem
1   The INSERM U 150, CNRS UA 334 - Hôpital Lariboisière, Paris, France
,
Sylvia Bellucci
1   The INSERM U 150, CNRS UA 334 - Hôpital Lariboisière, Paris, France
,
Jeannette Soria
1   The INSERM U 150, CNRS UA 334 - Hôpital Lariboisière, Paris, France
,
Claudine Soria
1   The INSERM U 150, CNRS UA 334 - Hôpital Lariboisière, Paris, France
,
Jacques Maclouf
1   The INSERM U 150, CNRS UA 334 - Hôpital Lariboisière, Paris, France
,
Jacques Caen
1   The INSERM U 150, CNRS UA 334 - Hôpital Lariboisière, Paris, France
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Publikationsverlauf

Received 23. November 1987

Accepted after revision 31. März 1988

Publikationsdatum:
30. Juni 2018 (online)

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Summary

We have investigated the effect of purified immunoglobulin G (IgG) on endothelial cell functions in 16 patients with lupus anticoagulant, 9 of whom had systemic lupus erythematosus (SLE). Spontaneous or thrombin-stimulated secretion of prostacyclin (PGI2) by cultured human endothelial cells from umbilical cord vein (HUVEC) was not inhibited by the patient’s IgG. Nor was spontaneous release of tissue plasminogen activator (t-PA) or of its inhibitor (PAI) modified in the presence of patient’s IgG.

The rate of activation of purified protein C (PC) by HUVEC in the presence of thrombin was significantly lowered by patient’s IgG or Fab’ fragment (inhibition of 43%). Neutralization of this effect was obtained by incubation of a greater quantity of phospholipids (phosphatidylcholine, phosphatidylserine) with the patient’s IgG. Activation of PC was also performed using purified rabbit thrombomodulin (TM) and a similar inhibition of the patient IgG was observed (inhibition of 48%) but the activation of Gla-domainless PC was not modified.

Key words

Lupus anticoagulant - Endothelial cells - Protein C - Thrombomodulin - Prostacyclin
 

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