Aging and Diabetes Increase the Aggregating Potency of Rat Skin Collagen Towards Normal Platelets

Summary

Acid-soluble collagen samples were prepared from individual skins of 24 month old rats (n = 8), 2 month old young controls (n = 8) and from 6 month old streptozotocin-diabetic rats (n = 5) and their age-matched controls (n = 10). Less collagen was obtained by acid extraction and salt precipitations from skins of diabetic and aged rats than from those of their respective controls. The collagen preparations from diabetic and aged rats showed an increased ratio of beta/alpha components. The rate of “in vitro” fibrillogenesis was less for collagen from diabetic rats than from controls. It was not modified for collagens from aged rats. The aggregating potency towards normal human platelets was markedly increased for collagens from aged and diabetic rats: reduced latency time (p <0.01) and increased velocity (p <0.01) were observed for collagens from aged rats when compared with young rats (16.5 μg/ml). Increased velocity (p <0.01) was also observed for collagens from diabetic rats (8.25, 11 and 16.5 μg/ ml), without modification of latency time.

Key words

Skin collagen - Platelet aggregation - Diabetes - Aging

• References

• 1 Heath M, Briden WD, Conever JV, Pollock J, Hunter PR, Kesley J, Bloom A. Platelet adhesiveness and aggregation in relation to diabetic retinopathy. Diabetologia 1971; 7: 308-315
• 2 Colwel JA, Haluskka PV, Sarji K, Levin J, Sagel J, Nair R. Altered platelet function in diabetic mellitus. Diabetes 1976; 27: 826-831
• 3 Scarabin PY, Samama M. Effect of age on ADP-induced platelet aggregation in diabetic and non-diabetic subjects. Thromb Res 1981; 22: 687-692
• 4 Balleinsen L, Rauterberg J. Platelet activation by basement membrane collagens. Thromb Res 1980; 18: 725-732
• 5 Santoro SA, Cunningham LW. The interaction of platelet with collagen. In: Platelets in Biology and Pathology. Gorden JL. (ed) North-Holland Biochemical Press; Amsterdam: 1981. 2 249-264
• 6 Le Pape A, Gutman N, Guitton JD, Legrand Y, Muh JP. Nonenzymatic glycosylation of collagen in diabetes: Incidence on increased normal platelet aggregation. Haemostasis 1983; 13: 36-41
• 7 Le Pape A, Gutman N, Guitton JD, Legrand Y, Muh JP. Nonenzymatic glycosylation increases platelet aggregating potency of collagen from placenta of diabetic human beings. Biochem Biophys Res Commun 1983; 2: 602-610
• 8 Kang AH, Nagai Y, Piez KA, Gross J. Studies on the structure of collagen utilizing a collagenolytic enzyme from Tadpole. Biochemistry 1966; 5 (02) 509-515
• 9 Woessner JJ. The determination of hydroxyproline in tissue and protein samples containing small proportions of this immunoacid. Arch J Biochem Biophys 1961; 93: 440-447
• 10 Weber K, Osborn M. The reliability of molecular determinations by dodecyl-sulfate polyacrilamide gel electrophoresis. J Biol Chem 1969; 16: 4406-4412
• 11 Born GV R. Aggregation of blood platelets by ADP and its reversal. Nature 1962; 194: 927-929
• 12 Guitton JD, Le Pape A, Sizaret PY, Muh JP. Effects of in vitro glycosylation on type I collagen fibrogenesis. Biosci Rep 1981; 1: 945-954
• 13 Amnudeswari S, Liang JN, Chakrabarti B. Polar-apolar characteristics and fibrilogenesis of glycosylated collagen. Collagen Rel Res 1987; 7: 215-223
• 14 Piez KA, Trus BL. Microfibrillar structure of packing of collagen hydrophobic interactions. J Mol Biol 1977; 110: 701-704
• 15 Golub LM, Greenwald RA, Zebrowski J, Ramamurthy NS. The effect of experimental diabetes on the molecular characteristics of soluble rat-tail tendon. Biochim Biophys Acta 1978; 534: 73-81
• 16 Fauvel F, Legrand YJ, Caen JP. Platelet adhesion to type I collagen and alpha₁ (I)₃ trimers: Involvement of the C-terminal alpha₁ CB6a peptide. Thromb Res 1978; 12: 273-285
• 17 Fauvel F, Legrand YJ, Kuhn K, Bentz H, Fietzek PP, Caen JP. Platelet adhesion to type III collagen: Involvement of a sequence of nine aminoacids from alpha₁ (III) CB4 peptide. Thromb Res 1979; 16: 269-273
• 18 Sternberg M, Grochulski A, Peyroux J, Hirbec G, Poirier J. Studies of the alpha glucosidase specific for collagen disaccharide units: variations associated with capillary basement membrane thickening in kidney and brain of diabetic and aged rats. Collagen Rel Res 1982; 2: 495-506
• 19 Sternberg M, Andre J, Peyroux J. Inhibition of the alpha glucosidase specific for collagen disaccharide units in diabetic rat kidney by in vivo glucose levels: Possible contribution to basement membrane thickening. Diabetologia 1983; 23: 286-289