Effect of Dextran on Experimental Venous Thrombosis in Rabbits

A.-K Ah-See; K.-E Arfors; D Bergqvist; O Tangen

doi:10.1055/s-0038-1647699

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1974; 32(02/03): 284-291
DOI: 10.1055/s-0038-1647699

Original Article

Schattauer GmbH

Effect of Dextran on Experimental Venous Thrombosis in Rabbits

A.-K Ah-See

¹Department of Experimental Medicine, Pharmacia AB and Department of Surgery, University Hospital, Uppsala, Sweden, and Department of Surgery, University of Aberdeen, Aberdeen, Scotland

,

K.-E Arfors

¹Department of Experimental Medicine, Pharmacia AB and Department of Surgery, University Hospital, Uppsala, Sweden, and Department of Surgery, University of Aberdeen, Aberdeen, Scotland

,

D Bergqvist

¹Department of Experimental Medicine, Pharmacia AB and Department of Surgery, University Hospital, Uppsala, Sweden, and Department of Surgery, University of Aberdeen, Aberdeen, Scotland

,

O Tangen

¹Department of Experimental Medicine, Pharmacia AB and Department of Surgery, University Hospital, Uppsala, Sweden, and Department of Surgery, University of Aberdeen, Aberdeen, Scotland

› Author Affiliations

Abstract

Summary

An experimental model combining controlled stasis and endothelial destruction by sodium morrhuate has been used to investigate the effect of dextran on venous thrombus formation in rabbits. Dextran 70 (1 g/kg body weight in a 10% solution), given 3 hours before thrombosis induction, significantly reduced the incidence of venous thrombosis when compared to a saline control. Dextran 40 (1 g/kg body weight in a 10% solution) and dextran 70 (0.6 g/kg body weight in a 6% solution) had no significant effect under these conditions. When given 10 or 120 minutes before initiation of thrombosis none of the dosages of dextran had any preventive effect. The results indicate that the haemodynamic effects of dextran were not involved in its preventive effect in this study. The effect of dextran was probably due to a combination of an effect on platelet reactivity and a change of the structure of fibrin formed during growth of the thrombi.

Full Text

References

References
1 Ahlberg Â, Nylander G, Robertson B, Cronberg S, Nilsson I. M. 1968; Dextran prophylaxis of thrombosis in fractures of the hip. Acta Chirurgica Scandinavica, Suppl 387: 83.
2 Arfors K-E, Bergqvist D, Davidson A. Influence of dextran on the arteriolar haemostatic plug formation. To be published..
3 Arfors K-E, Dhall D. P, Engeset J, Hint H, Matheson N. A, Tangen O. 1969. In vivo quantitation of platelet activity using biolaser-induced endothelial injury. 5th European Conference on Microcirculation. Gothenburg. 1968. Karger, Basel: 502.
4 Arfors K-E, Hint H. C, McKenzie F. N, Matheson N. A, Svensjö E. 1970; Counteraction of platelet activity using laser induced entodhelial trauma. Thrombosis et Diathesis Haemorrhagica, Suppl 42: 315.
5 Berman H. 1964; Platelet agglutinability as a factor in hemostasis. Biblioteca Ana- tomica 04: 736.
6 Berman H, Fulton G. 1961. Platelets in the peripheral circulation. In: Henry Ford Hospital International Symposium on Blood Platelets. Detroit: 1960: 7.
7 Borgström S, Gelin L-E, Zederfeldt B. 1959; The formation of vein thrombi following tissue injury. An experimental study in rabbits. Acta Chirurgica Scandinavica Suppl. 247.
8 Bygdeman S, Eliasson R. 1967; Effect of dextrans on platelet adhesiveness and aggregation. Scandinavian Journal of Clinical and Laboratory Investigation 20: 17.
9 Bygdeman S, Eliasson R, Gullbring B. 1966; Effect of dextran infusion on the adenosine diphosphate induced adhesiveness and the spreading capacity of human blood platelets. Thrombosis et Diathesis Haemorrhagica 15: 451.
10 Cronberg S, Robertson B, Nilsson I. M, Nihlén J-E. 1966; Suppressive effect of dextran on platelet adhesiveness. Thrombosis et Diathesis Haemorrhagica 16: 384.
11 French J. E. 1969. The fine structure of experimental thrombi. In: Sherry S, Brinkhotjs K, Genton E, Stengle I. (eds.), Thrombosis. National Academy of Sciences; Washington D.C: 1969
12 Golltjb S, Schaefer C. 1968; Structural alteration in canine fibrin produced by colloid plasma expanders. Surgery, Gynaecology and Obstetrics 127: 783.
13 Grossi C, Cliffton E, Cannamela D. 1954; The lysis of intravascular thrombi in rabbits with human plasmin (fibrinolysin). Blood 09: 310.
14 Henry R. 1962; Methods for inducing experimental thrombosis. An annotated bibliography. Angiology 13: 554.
15 Johnson S. R, Bygdeman S, Eliasson R. 1968; Effect of dextran on postoperative thrombosis. Acta Chirurgica Scandinavica, Suppl 387: 80.
16 Koekenberg L. J. L. 1961; Experimental use of Macrodex as a prophylaxis against post-operative thromboembolism. Excerpta Medica 40: 123.
17 Lambie J. M, Barber D. C, Dhall D. P, Matheson N. A. 1970; Dextran 70 in prophylaxis of post-operative venous thrombosis. A controlled trial. British Medical Journal 02: 144.
18 Luft J. H. 1961; Improvements in epoxy resin embedding methods Journal of Biophysical and Biochemical Cytology. 09: 409.
19 Muzaffar T. Z, Stalker A. L, Bryce W. A. J, Dhall D. P. 1972; Structural alterations in fibrin clots with dextran. Thrombosis et Diathesis Haemorrhagica 28: 257.
20 Peterson J, Zucker M. 1970; The effect of adenosine monophosphate, arcaine and anti-inflammatory agents on thrombosis and platelet function in rabbits. Thrombosis et Diathesis Haemorrhagica 23: 148.
21 Reiner L. 1944; The activity of anionic surface active compounds in producing vascular obliteration. Proceedings of the Society for Experimental Biology and Medicine 62: 49.
22 Tangen O, Wik K. O, Almqvist I. A. M, Arfors K-E, Hint H. C. 1972; Effects of dextran on the structure and plasmin-induced lysis of human fibrin. Thrombosis Research 01: 487.
23 Wright H. P, Kubik M. M, Hayden M. 1952; Influence of anticoagulant administration on the rate of recanalization of experimentally thrombosed veins. British Journal of Surgery 40: 163.