Measurement of the Platelet Response to Laserinduced Microvascular Injury

T Hovig; F. N McKenzie; K.-E Arfors

doi:10.1055/s-0038-1647739

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1974; 32(02/03): 695-703
DOI: 10.1055/s-0038-1647739

Original Article

Schattauer GmbH

Measurement of the Platelet Response to Laserinduced Microvascular Injury

Ultrastructural Studies

T Hovig

¹Dept, of Pathology, Rikshospitalet, University of Oslo, Oslo, Norway, Dept, of Surgery, Univ. of Aberdeen, Aberdeen, Scotland and Dept, of Experimental Medicine, Pharmacia AB, Uppsala, Sweden

,

F. N McKenzie

¹Dept, of Pathology, Rikshospitalet, University of Oslo, Oslo, Norway, Dept, of Surgery, Univ. of Aberdeen, Aberdeen, Scotland and Dept, of Experimental Medicine, Pharmacia AB, Uppsala, Sweden

,

K.-E Arfors

¹Dept, of Pathology, Rikshospitalet, University of Oslo, Oslo, Norway, Dept, of Surgery, Univ. of Aberdeen, Aberdeen, Scotland and Dept, of Experimental Medicine, Pharmacia AB, Uppsala, Sweden

› Author Affiliations

Abstract

Summary

Microvascular injury was produced in rabbit mesentery and ear chamber preparations using a biolaser. Ultrastructural studies of the injury sites revealed damage to the endothelium which showed cytoplasmic swelling and flocculation. Complete destruction of the endothelium was not observed, indicating that sub endothelial structures were not exposed to blood flow. Microvascular thrombi which occurred at the sites of injury were composed of platelets which in general were loosely packed and showed minor alterations in shape and internal structure. Erythrocytes appeared to be heavily damaged by the laser injury. Fibrin was not observed. It is concluded that ADP may be released from red blood cells, and possibly from other sources, as a result of the laser injury, and that the platelet thrombus formation is induced by ADP.

Full Text

References

References
1 Arfors K.-E, McKenzie F. N, Matheson N. A. 1975 Measurement of the platelet response to laser-induced microvascular injury: influence of haematological and haemodynamic variables. Thrombosis Research. in Press.
2 Hovig T. 1968; The ultrastructure of blood platelets in normal and abnormal states. Series Haematologica 01: 3.
3 McKenzie F. N, Arfors K-E. 1975 Measurement of the platelet response to laser-induced microvascular injury; methodological studies. Thrombosis Research. in Press.
4 McKenzie F. N, Areors K-E, Matheson N. A. 1975; Measurement of the platelet response to laser-induced microvascular injury; assessment of determinants of platelet aggregation in vivo. Thrombosis et Diathesis Haemorrhagica 32: 704.