Download PDF
Thromb Haemost 1973; 29(01): 063-075
DOI: 10.1055/s-0038-1647746
Original Article
Schattauer GmbH

Factors Regulating Plasma Protein Synthesis

II. Influence of Fibrinogenolytlc Products on Plasma Fibrinogen Concentration
V Bocci
1   Istituto di Fisiologia Generate dell’ Università di Siena 53100, Siena, Italy
,
A Pacini
1   Istituto di Fisiologia Generate dell’ Università di Siena 53100, Siena, Italy
› Author Affiliations
Further Information

Publication History

Received 28 April 1972

Publication Date:
30 June 2018 (online)

Also available at
    Permissions and Reprints

Summary

The intravenous injection of a large amount of homologous fibrinogen in the rabbit produces typical changes in the level of circulating fibrinogen. The first peak is due to the administered fibrinogen while the second, occurring after 16 hr, is interpreted as being due to the stimulation of hepatic synthesis and release of preformed fibrinogen. This typical profile was obtained with different fibrinogen preparations but not with heterologous fibrinogen. Neither was it obtained after administration of homologous γ-globulin, α-globulin or albumin. Assuming that enhancement of hepatic synthesis might be due to fibrinogenolytic products, the effect of the injection of fibrinogen digest products (FDP) was investigated. Administration of homologous FDP causes, within 24 hours, a significant increase of the plasma fibrinogen level. The critical requirement of injecting either the protein solution or saline intravenously rather than subcutaneously is emphasized. In conclusion, FDP appear to be endowed with the novel property of stimulating fibrinogen synthesis and possibly of causing the release of preformed fibrinogen.

 

  • References

  • 1 Atencio A. C, Chao P-Y, Chen A. Y, Reeve E. B. 1969; Fibrinogen response to corticotropin preparations in rabbits. American Journal of Physiology 216: 773.
    PubMedSearch in Google Scholar
  • 2 Atencio A. C, Joiner K, Reeve E. B. 1969; Experimental and control systems studies of plasma fibrinogen regulation in rabbits. American Journal of Physiology 216: 764.
    PubMedSearch in Google Scholar
  • 3 Atencio A. C, Loeand L. 1970; Effect of ACTH on biosynthesis of fibrinogen in the rabbit. American Journal of Physiology 219: 1161.
    PubMedSearch in Google Scholar
  • 4 Baenhaet M. I, Cress D. C, Noonan S. M, Walsh R. T. 1970. Influence of fibrinolytic products on hepatic release and synthesis of fibrinogen. In: Owren P. A, Brinkhotjs K. M, Wright I. S, Roberts H. R, Hinnom S, Kiesselbach T. H. Fibrinogen: Structural, Metabolic and Pathophysiologic Aspects. F. K. Schattauer Verlag: Stuttgart; 143.
    Search in Google Scholar
  • 5 Bocci V. 1970; Metabolism of plasma proteins. Archivio di Fisiologia 67: 314.
    PubMedSearch in Google Scholar
  • 6 Bocci V, Pacini A. 1971; Factors regulating the synthesis of plasma fibrinogen. Proceedings of the International Union of Physiological Sciences. XXV International Congress, Munich July 25-31, 1971, vol. 9: 70.
    PubMedSearch in Google Scholar
  • 7 Bocci V, Viti A. 1971; Factors regulating plasma protein synthesis. I. Circadian fluctuations of plasma fibrinogen mass in the rat. American Journal of Physiology 221: 719.
    PubMedSearch in Google Scholar
  • 8 Chandler A. M, Snider G. A. 1970; Plasma protein biosynthesis following laparotomy and partial hepatectomy. Proceedings of the Society for Experimental Biology and Medicine 135: 415.
    PubMedSearch in Google Scholar
  • 9 Chattopadhyay D. P, Cliffton E. E. 1965; Activation of rabbit plasminogen by streptokinase. American Journal of Physiology 208: 190.
    PubMedSearch in Google Scholar
  • 10 Dudek G. A, Kloczewiak M, Budzynski A. Z, Latallo Z. S, Kopec M. 1970; Characterisation and comparison of macromolecular and products of fibrinogen and fibrin proteolysis by plasmin. Biochimica et Biophysica Acta 214: 44.
    CrossrefPubMedSearch in Google Scholar
  • 11 Fisher R. A, Yates F. 1953. Statistical tables for biological, agricultural and medical research. . Oliver and Body; London.:
    Search in Google Scholar
  • 12 Fletcher A. P, Alkjaersig N, Sherry S. 1962; Pathogenesis of the coagulation defect developing during pathological plasma proteolytic (“fibrinolytic”) states. I. The significance of fibrinogen proteolysis and circulating fibrinogen breakdown products. Journal of Clinical Investigation 41: 896.
    CrossrefPubMedSearch in Google Scholar
  • 13 Freeman T. 1965; Gamma globulin metabolism in normal humans and in patients. Scandinavian Journal of Haematology Suppl. 4, 76.
    PubMedSearch in Google Scholar
  • 14 Gans H, Lowman J. T. 1967; The uptake of fibrin and fibrin-degradation products by the isolated perfused rat liver. Blood 29: 526.
    PubMedSearch in Google Scholar
  • 15 Gornall A. G, Bardawill C. J, David M. M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177: 751.
    PubMedSearch in Google Scholar
  • 16 Hilgard P, Hiemeyer V. 1970; Increased plasma fibrinogen and the release of a fibrinogen enhancing factor in tumour-bearing rats. Experientia 26: 182.
    CrossrefPubMedSearch in Google Scholar
  • 17 Hombtjrger F. 1945; A plasma fibrinogen-increasing factor obtained from sterile abscesses in dogs. Journal of Clinical Investigation 24: 43.
    CrossrefPubMedSearch in Google Scholar
  • 18 Jamieson G. A, Gaffney Jr P. J.. 1968; Nature of the high molecular weight fraction of fibrinolytic digests of human fibrinogen. Biochimica et Biophysica Acta 154: 96.
    CrossrefPubMedSearch in Google Scholar
  • 19 Jeejeebhoy K. N, Bruce-Robertson A, Sodtke U, Foley M. 1970; The effect of growth hormone on fibrinogen synthesis. Biochemical Journal 119: 243.
    CrossrefPubMedSearch in Google Scholar
  • 20 John D. W, Miller L. L. 1969; Regulation of net biosynthesis of serum albumin and acute phase plasma proteins. Journal of Biological Chemistry 244: 6134.
    PubMedSearch in Google Scholar
  • 21 Koj A. 1970. Synthesis and turnover of acute-phase reactants. In: Porter R, Knight J. (eds.) Energy Metabolism in Trauma. Ciba Foundation Symposium. Churchill; London: 79.
    Search in Google Scholar
  • 22 Koj A. 1970; Acute-phase reactants and lysosomal enzymes in the blood of rats with experimental inflammation or radiation injury. Folia Biologica 18: 275.
    PubMedSearch in Google Scholar
  • 23 Kowalski E. 1968; Fibrinogen derivatives and their biologic activities. Seminars in Hematology 05: 45.
    PubMedSearch in Google Scholar
  • 24 Kukeal J. C, Zeineh R, Dobryszycka W, Pollitt J, Stone N. 1969; Metabolism of plasma proteins in injury states. Clinical Science 36: 221.
    PubMedSearch in Google Scholar
  • 25 Lerner R. G, Rapaport S. I, Siemsen J. K, Spitzer J. M. 1968; Disappearance of fibrinogen-131I after endotoxin: effects of a first and second injection. American Journal of Physiology 214: 532.
    PubMedSearch in Google Scholar
  • 26 Marder V. J, Shtjlman N. R, Carroll W. R. 1969; High molecular weight derivatives of human fibrinogen produced by plasmin. I. Physicochemical and immunological characterization. Journal of Biological Chemistry 244: 2111.
    PubMedSearch in Google Scholar
  • 27 McFarlane A. S. 1963; In vivo behavior of I131fibrinogen. Journal of Clinical Investigation 42: 346.
    CrossrefPubMedSearch in Google Scholar
  • 28 McFarlane A. S, Todd D, Cromwell S. 1964; Fibrinogen catabolism in humans. Clinical Sciences 26: 415.
    PubMedSearch in Google Scholar
  • 29 Merskey C. 1969; The quantitation of fibrinogen or fibrin split products. Thrombosis et Diathesis Haemorrhagica Suppl. 36, 195.
    PubMedSearch in Google Scholar
  • 30 Merskey C, Lalezari P, Johnson A. J. 1969; A rapid, simple, sensitive method for measuring fibrinolytic split products in human serum. Proceedings of the Society for Experimental Biology and Medicine 131: 871.
    PubMedSearch in Google Scholar
  • 31 Miller L. L, Hanavan H. R, Titthasiri N, Chowdhury A. 1964; Dominant role of the liver in the biosynthesis of the plasma proteins with special reference to the plasma muco-proteins (seromucoid) ceruloplasmin and fibrinogen. Chemistry 44: 17.
    PubMedSearch in Google Scholar
  • 32 Monkhouse F. C, Milojevic S. 1960; Changes in fibrinogen level after infusion of thrombin and thromboplastin. American Journal of Physiology 199: 1165.
    PubMedSearch in Google Scholar
  • 33 Pacini A, Bocci V. 1970; An evaluation of methods for determining the concentration of fibrinogen in rabbit plasma. Italian Journal of Biochemistry 19: 241.
    PubMedSearch in Google Scholar
  • 34 Pessina G. P, Bocci V. 1971; Effect of hypoxia on plasma fibrinogen concentration in rabbits. Archivio di Fisiologia 68: 172.
    PubMedSearch in Google Scholar
  • 35 Pessina G. P, Conti T, Pacini A, Bocci V. 1972 Factors regulating plasma protein synthesis. III. Large scale separation of rabbit fibrinogenolytic products Italian Journal of Biochemistry. in the press..
    PubMedSearch in Google Scholar
  • 36 Pilgeram L. O, Pickart L. R. 1968; Control of fibrinogen biosynthesis: the role of free fatty acid. Journal Atherosclerosis Research 08: 155.
    PubMedSearch in Google Scholar
  • 37 Regoeczi E. L, Henley G. E, Holloway R. C, McFarlane A. S. 1963; Turnover of 131I-labelled fibrinogen in fever. British Journal of Experimental Pathology 44: 397.
    PubMedSearch in Google Scholar
  • 38 Regoeczi E. 1970; Fibrinogen catabolism: kinetics of catabolism following sudden elevation of the pool with exogenous fibrinogen. Clinical Science 38: 111.
    CrossrefPubMedSearch in Google Scholar
  • 39 Regoeczi E. 1971; Iodine-Labelled Fibrinogen: A review. British Journal of Haematology 20: 649.
    CrossrefPubMedSearch in Google Scholar
  • 40 Sherman L. A, Fletcher A. P, Sherry S. 1969; In vivo transformation between fibrinogen of varying ethanol solubilities: A pathway of fibrinogen catabolism. Journal of Laboratory and Clinical Medicine 73: 574.
    PubMedSearch in Google Scholar
  • 41 Siegel N. J, Kolmen S. N. 1969; Fibrinogen: reversible sequestration associated with acidosis in situ. American Journal of Physiology 216: 707.
    PubMedSearch in Google Scholar
  • 42 Snedecor G. W, Cochran W. G. 1968. Statistical methods. . The Iowa State University Press Ames; Iowa, USA.:
    Search in Google Scholar
  • 43 Thuot C, Larrieu M. J. 1971; Fibrinogen degradation products: a critical study of two assay methods. Revue Europeenne d’Etudes Cliniques et Biologiques 16: 27.
    PubMedSearch in Google Scholar
  • 44 Triantaphyllopoulos D. C. 1960; Effect of intravenous injections of the anticoagulant fraction of incubated fibrinogen on blood coagulation. Canadian Journal of Biochemistry and Physiology 38: 909.
    CrossrefPubMedSearch in Google Scholar
  • 45 Walsh R. T, Baenhaet M. I. 1969. Clearance of coagulation and fibrinolysis products by the reticuloendothelial system. In: Mammen E. F, Anderson G. F, Baenhakt M. I. (eds.). Disseminated Intravascular Coagulation. . F. K. Schattauer Verlag; Stuttgart: 83.
    Search in Google Scholar
  • 46 Wycofe H. D. 1970; Production of fibrinogen following an endotoxin injection. Proceedings of the Society for Experimental Biology and Medicine 133: 940.
    PubMedSearch in Google Scholar
  • 47 Young M. C, Kolmen S. N. 1968; Sustained lymphatic delivery of fibrinogen after induced afibrinogenemia. Thrombosis et Diathesis Haemorrhagica 19: 198.
    PubMedSearch in Google Scholar
  • 48 Young M. C, Kolmen S. N. 1970; Recovery of fibrinogen in fibrinolytic dogs. Thrombosis et Diathesis Haemorrhagica 23: 50.
    PubMedSearch in Google Scholar