The Young Platelet Population in Children with Cyanotic Congenital Heart Disease

Béla Goldschmidt; Svein Jan Sørland

doi:10.1055/s-0038-1647927

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1976; 35(02): 342-349
DOI: 10.1055/s-0038-1647927

Original Article

Schattauer GmbH

The Young Platelet Population in Children with Cyanotic Congenital Heart Disease

Authors

Béla Goldschmidt

¹Second Department of Paediatrics, Semmelweis Medical University, Budapest, Hungary, Institute for Thrombosis Research and Department of Paediatrics, Rikshospitalet, Oslo, Norway
Svein Jan Sørland

¹Second Department of Paediatrics, Semmelweis Medical University, Budapest, Hungary, Institute for Thrombosis Research and Department of Paediatrics, Rikshospitalet, Oslo, Norway

Abstract

Summary

Some morphological, biochemical and functional parameters of platelet population in children with cyanotic congenital heart disease (CCHD) were studied by making comparisons of the normal platelet population in both CCHD patients and controls. The mean volume of the platelets from cyanotic patients was greater than from normals. The platelet size distribution curves demonstrated a shift towards larger than normal size in the case of CCHD. The mean protein content, as well as the mean PF3 content of platelets was increased in CCHD. Following addition of kaolin, PF3 release was more rapid and of shorter duration with platelets from CCHD patients as compared to normal platelets. They also released more PF3 than did normal platelets. After addition of ADP, collagen, or adrenalin, platelets of CCHD patients were more responsive than similarly treated platelets from normals. Platelets from CCHD showed an increased initial rate of aggregation and greater maximum aggregation. These data suggested that the platelet population of CCHD patients consists of larger, younger and functionally more active platelets than does the platelet population of normals.

Full Text

References

References
1 Born G. V. R. 1962; Quantitative investigations into the aggregation of blood platelets. Journal of Physiology 762: 67.
2 Bøyum A. 1962; Separation of leukocytes from blood and bone marrow. Scandinavian Journal of Clinical and Laboratory Investigation, Supplement 21: 97.
3 Detwiler T. C, Odell Jr. T. T, McDonald T. P. 1962; Platelet size, ATP content and clot retraction in relation to platelet age. American Journal of Physiology 203: 107.
4 Feissly R, Lüdin H. 1949; Microscopie par contrastes de phases; applications a l’hématologie. Revue d’hématologie 4: 481.
5 Ginsburg A. D, Aster R. H. 1970; Changes associated with platelet aging. Thrombosis et Diathesis Haemorrhagica 23: 407.
6 Goldschmidt B. 1970; Blutkoagulationsstörungen bei Kindern mit angeborenen Herzfehlern. Monatsschrift fur Kinderheilkunde 118: 450.
7 Goldschmidt B, Fonó R. 1972; Cyclic fluctuations in platelet count, megakaryocyte maturation and thrombopoietin activity in cyanotic congenital heart disease. Acta Paediatrica Scandinavica 61: 310.
8 Goldschmidt B, Sarkadi B, Gárdos G, Matlary A. 1974; Platelet production and survival in cyanotic congenital heart disease. Scandinavian Journal of Haematology 13: 110.
9 Grossman C. M, Kohn R. 1965; Enzymatic characteristics of in vitro incorporation of P³² orthophosphate into human platelet phosphatides. Thrombosis et Diathesis Haemorrhagica 13: 126.
10 Holmsen H, Ostvold A. C, Day H. J. 1973; Behaviour of endogenous and newly absorbed serotonin in the platelet release reaction. Biochemical Pharmacology 22: 2599.
11 Ihenacho H. N. C, Breeze G. P, Fletcher G. J, Stuart J. 1973; Consumption coagulopathy in congenital heart disease. Lancet 1: 231.
12 Inceman S, Tangün Y. 1969; A simple test for the evaluation of platelet coagulant activity. Journal of Laboratory and Clinical Medicine 74: 174.
13 Karpatkin S. 1969; Heterogenity of human platelets II. Functional evidence suggestive of young and old platelets. Journal of Clinical Investigations 48: 1083.
14 Karpatkin S. 1972; Human platelet senescence. Annual Review of Medicine 23: 101.
15 Lövry O. H, Rosenbrough N. J, Farr A. L, Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265.
16 Maurer H. M, McCue C. M, Caul J, Still W. J. S. 1972; Impairement in platelet aggregation in cyanotic congenital heart disease. Blood 40: 207.
17 McDonald T. P, Odell Jr. T. T, Gosslee D. G. 1964; Platelet size in relation to platelet age. Proceedings of the Society for experimental Biology and Medicine 115: 684.
18 Miller G. L. 1959; Protein determinations for large numbers of samples. Analytical Chemistry 31: 964.
19 O’Brien J. R. 1973. Drug induced thrombocytopathy. In: Girdwood R. H. (ed.) Blood disorders due to drugs and other agents . Excerpta Medica Foundation; Amsterdam:
20 Spaet T. H, Cintron J. 1965; Studies on platelet factor 3 availability. British Journal of Haematology 11: 269.
21 Stormorken H, Lund-Ruse A, Røvik T. O. 1965; Platelet adhesiveness to glass beads: methodological investigations using automatic platelet counting. Scandinavian Journal of Clinical and Laboratory Investigation. Supplement 85: 184.