Importance of Protease Inhibition in Studies on Purified Factor VIII (Antihaemophilic Factor)

Eugen A. Beck; Peter Bachmann; Peter Barbier; Miha Furlan

doi:10.1055/s-0038-1647943

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1976; 35(01): 186-190
DOI: 10.1055/s-0038-1647943

Original Article

Schattauer GmbH

Importance of Protease Inhibition in Studies on Purified Factor VIII (Antihaemophilic Factor)

Authors

Eugen A. Beck

¹Central Haematology Laboratory, Inselspital, CH-3010 Berne, Switzerland
Peter Bachmann

¹Central Haematology Laboratory, Inselspital, CH-3010 Berne, Switzerland
Peter Barbier

¹Central Haematology Laboratory, Inselspital, CH-3010 Berne, Switzerland
Miha Furlan

¹Central Haematology Laboratory, Inselspital, CH-3010 Berne, Switzerland

Abstract

Summary

According to some authors factor VIII procoagulant activity may be dissociable from carrier protein (MW~ 2 × 10⁶) by agarose gel filtration, e.g. at high ionic strength. We were able to reproduce this phenomenon. However, addition of protease inhibitor (Trasylol) prevented the appearance of low molecular weight peak of factor VIII procoagulant activity both at high ionic strength and elevated temperature (37°C). We conclude from our results that procoagulant activity and carrier protein (von Willebrand factor, factor VIII antigen) are closely associated functional sites of native factor VIII macro molecule. Consequently, proteolytic degradation should be avoided in functional and structural studies on factor VIII and especially in preparing factor VIII concentrate for therapeutic use.

Full Text

References

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