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DOI: 10.1055/s-0038-1647966
On the Measurement of Spontaneous Platelet Aggregation. The Platelet Aggregation Test III.
Methods and First Clinical ResultsPublikationsverlauf
Received:
30. April 1974
Accepted:
28. November 1976
Publikationsdatum:
02. Juli 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/197601/lookinside/thumbnails/10-1055-s-0038-1647966-1.jpg)
Summary
A new measuring device was developed for the study of “spontaneous” aggregating activity of thrombocytes. In the photometric platelet aggregation test (PAT III) 0.6 ml of platelet-rich plasma (PRP) are rotated in a disc-shaped cuvette at 20 rpm and 37° C. Changes in optical density of PRP which are induced by the formation of platelet aggregates are continuously registered using a chart recorder. PAT III was developed for the detection of enhanced platelet aggregation, indicating a risk of thrombosis and thromboembolic complications.
In 146 healthy individuals a certain percentage showed slight primary aggregation (α1) which in some cases was followed by marked aggregation (α2) at a certain time (Tr) after the beginning of rotation. The percentage of individuals showing α2 increased with age. An increase of plasma pH in the rotating sample, which was caused by diffusion of CO2, was an important conditioning factor for aggregation. The test results depended on the platelet count in PRP. Aggregation curves were suppressed by admixture of erythrocytes and lipid turbidity. The tendency of platelets to aggregate increased within 60–90 min following blood sampling. During this period the interval to the onset of aggregation (Tr) became shorter and the maximum aggregation speed (α2) increased with time. PAT III yielded reproducible results when it was carried out more than 60 min after blood drawing.
In a group of 327 diabetic patients “spontaneous” aggregation occurred more frequently in all age groups as compared with the controls.
Additional equipment was available for the registration of ADP-, collagen-, or epine-phrine-induced aggregation similar to Born’s and O’Brien’s method. The device can easily be mounted on an Eppendorf photometer without further alterations.
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References
- 1 Badawi H, El Sawy M, Mikhail M, Nomeir A. M, Tewfik S. 1970; Platelets, coagulation and fibrinolysis in diabetic and nondiabtic patients with quiescent coronary disease. Angiology 21: 511.
- 2 Born G. V. R. 1962; Quantitative investigations into the aggregation of blood platelets. Journal of Physiology 162: 67.
- 3 Breddin K. 1963. Untersuchungen über die Agglutinationsbereitschaft der Thrombozyten bei Gefäßkrankheiten. In: Emmrich R, Perlick E. Gefäßwand und Blutplasma. IL Symp. Med; Klinik Leipzig:
- 4 Breddin K. 1968 Die Thrombozytenfunktion bei hämorrhagischen Diathesen, Thrombosen und Gefäßkrankheiten. Thrombosis et Diathesis Haemorrhagica. Suppl. 27.
- 5 Breddin K. 1968; Klinische und experimentelle Untersuchungen über die Thrombozytenaggregation bei Gefäßerkrankungen. Deutsche medizinische Wochenschrift 93: 1555.
- 6 Breddin K, Bauke J. 1965; Thrombozytenagglutination und Gefäßkrankheiten. Blut 11: 144.
- 7 Breddin K, Krzywanek H. J, Scharrer I, Jäger W. 1973. Heparinoids, Sulfated Polysaccharides and Platelet Aggregation. IInd International Symposium, Vienna, lune 1972, printed in Erythrocytes, Thrombocytes, Leukocytes. Georg Thieme Publishers; Stuttgart:
- 8 Breddin K, Krzywanek H. J, Bald M, Kutschera J. 1974. Is enhanced platelet aggregation a risk factor for thromboembolic complications in atherosclerosis?. In: Schettler G. U, Weizel A. Atherosclerosis III. Springer Verlag; Heidelberg, New York: 197.
- 9 Breddin K, Krzywanek H. J, Ziemen M. 1975; Methoden zur Erfassung einer Thromboseneigung oder zur Erkennung arterieller Gefäßprozesse. Therapiewoche 25: 1558.
- 10 Breddin K, Ziemen M, Bauer O, Schaudinn L, Ludwig H. Time dependent changes of ADP-collagen or epinephrineinduced and “spontaneous” platelet aggregation and platelet morphology. In preparation.
- 11 Fyfe T, Hamilton E. 1967; Effect of variation of the interval between venepuncture and measurement of platelet adhesiveness by the Payling Wright method. Lancet II: 542.
- 12 Haanen C, Holdrinet A. 1968; Blood platelet aggregation under various conditions and in some diseases. Experimental Biology and Medicine 3: 164.
- 13 Hassanein A. A, El-Garf Th. A, El-Baz Z. 1972; Platelet aggregation in diabetes mellitus and the effect of insulin in vivo on aggregation. Thrombosis et Diathesis Haemorrhagica (Stuttg.), 27: 114.
- 14 Heath H, Bridgen W. D, Canever J. V, Pollock J, Hunter P. R, Kelsey J, Bloom A. 1971; Platelet adhesiveness in relation to diabetic retinopathy. Diabetology 7: 308.
- 15 Hellem J. 1971; Adenosinediphosphate induced platelet adhesiveness in diabetes mellitus with complications. Acta medica Scandinavica 190: 291.
- 16 Jäger W, Kutschera J, Wendeberg H, Kauschmann R, Riese W, Pietsch U, Berger E, Bennert C. 1974; Die fortlaufende Messung der Plättchenaggregation ohne Zugabe von Aggregationsauslösern. Blut 29: 184.
- 17 Kwaan H. C, Colwell J. A, Cruz S, Suwanwela N, Dobbie J. G. 1972; Increased platelet aggregation in diabetes mellitus. Journal of Laboratory and Clinical Medicine 80: 236.
- 18 Krzywanek H. J, Breddin K. 1971; Alterung und Einengung des plättchenaggregierenden Plasmafaktors. Verhandlung der Deutschen Gesellschaft für innere Medizin 77: 152.
- 19 Leuschner M, Breddin K. Electronmicroscopic investigations on the primary shape change of platelets at room temperature, in preparation..
- 20 O’Brien J. R. 1961; The adhesiveness of native platelets and its prevention. Journal of Clinical Pathology 14: 140.
- 21 Passa P, Bensossan D, Levy-Toledano S, Caen J, Canivet J. 1974; Etude de l’aggrégation plaquettaire au course de la rétinopathie diabétique. Atherosclerosis 19: 277.
- 22 Reuter H, Podolsak W, Hagen I, Linker H, Ströder J, Gross R. 1973; Investigations on the time dependance of platelet functions I. General methodical investigations. Thrombosis Research 3: 307.
- 23 Rogers A. B. 1972; The effect of pH on human platelet aggregation induced by epinephrine. Proceedings of the Society for Experimental Biology and Medicine 139: 1100.
- 24 Seitz L. 1966. Untersuchungen mit dem Plättchenaggregationstest nach Breddin und Bauke, vorwiegend bei peripheren Durchblutungsstörungen mit besonderer Berücksichtigung der Marcumar-behandlung und des Rauchens. Dissertationsschrift; München:
- 25 Skoza L, Zucker M. B, Jerushalmy Z, Grant R. 1967; Kinetic studies of platelet aggregation induced by adenosine diphosphate and its inhibition by chelating agents, guanidino compounds, and adenosine. Thrombosis et Diathesis Haemorrhagica 18: 713.
- 26 Warlow Ch, Corina A, Ogston D, Douglas A. S. 1974; The relationship between platelet aggregation and time interval after venepuncture. Thrombosis et Diathesis Haemorrhagica 31: 133.
- 27 Wessely S. 1972. Zur Methode des Plättchenaggregationstestes. Behandlung des Blutes zwischen Entnahme und Bestimmung. Dissertationsschrift; Frankfurt: