γ-Dimerization, α-Polymerization, and Plasmin Degradation of Human Fibrin

J Carsten Feddersen; Johs Gormsen

doi:10.1055/s-0038-1648006

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1976; 36(01): 027-036
DOI: 10.1055/s-0038-1648006

Original Article

Schattauer GmbH

γ-Dimerization, α-Polymerization, and Plasmin Degradation of Human Fibrin

Effect of Various Inhibitors of Factor XIII on the Patterns in SDS-Electrophoresis and Crossed Immunoelectrophoresis

Authors

J Carsten Feddersen

¹Coagulation Laboratory, Sundby Hospital, DK-2300 Copenhagen S, Denmark
Johs Gormsen

¹Coagulation Laboratory, Sundby Hospital, DK-2300 Copenhagen S, Denmark

Abstract

Summary

The effect of different factor XIII inhibitors (competetive inhibition, interference with active center SH-groups in different ways, Ca²⁺ depletion) on the sequence of the γ-dimerization and α-polymerization of fibrin is examined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAE) (reduced material). They all inhibit either γ-dimerization and a-polymerization or a-polymerization alone irrespective of the factor XIII inhibitory mechanism.

Non-crosslinked fibrin and fibrin clots of different degrees of crosslinking are digested with plasmin and the lysate tested in SDS-PAE (non-reduced material) and crossed agarose gel Immunoelectrophoresis (CAIE). The digests contain Fragment D and Fragment D-D respectively and Fragment E. An additional Fragment E with less anodic mobility in CAIE, and not demonstrable in SDS-PAE, is seen in increasing amounts with increasing γ-dimerization, α-polymerization does not further change the CAIE patterns.

Full Text

References

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