Download PDF
Thromb Haemost 1976; 36(02): 334-342
DOI: 10.1055/s-0038-1648049
Original Article
Schattauer GmbH

Sedimentation and Adhesion of Platelets onto a Horizontal Glass Surface

E Ruckenstein
1   Faculty of Engineering and Applied Sciences and the Department of Surgery, School of Medicine, State University of New York at Buffalo, Buffalo, NY 14214, U.S.A.
,
A Marmur
1   Faculty of Engineering and Applied Sciences and the Department of Surgery, School of Medicine, State University of New York at Buffalo, Buffalo, NY 14214, U.S.A.
,
S. R Rakower
1   Faculty of Engineering and Applied Sciences and the Department of Surgery, School of Medicine, State University of New York at Buffalo, Buffalo, NY 14214, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 10 January 1976

Accepted 18 May 1976

Publication Date:
03 July 2018 (online)

Also available at
    Permissions and Reprints

Summary

An experimental method for measuring platelet adhesion to a glass surface and platelet sedimentation rate is described. Anticoagulated platelet-rich plasma is placed on a horizontal glass slide for various contact periods. The number of platelets adhering to the slide per unit area is recorded as a function of time. The experimental results are used to verify theoretical predictions which account for the effect of the slow sedimentation rate of the platelets and for their escape over the potential barrier between them and the solid surface. By a least-squares fit of the theoretical equation to the experimental data, both the platelet adhesiveness, in terms of P the probability to overcome the potential barrier, and the platelet sedimentation rate V are evaluated. A range of values of P and V for healthy humans is presented.

 

  • References

  • 1 Curtis A. S. G. 1973; In: Butler and Noble (Eds.) Cell Adhesion. Progress in Biophysics and Molecular Biology 27 Pergamon Press 315.
    CrossrefPubMedGoogle Scholar
  • 2 George J. N, Weed R. I, Reed C. F. 1971; Adhesion of Human Erythrocytes to Glass: The Nature of the Interaction and the Effect of Serum and Plasma. Journal of Cell. Physiology 77: 51.
    CrossrefPubMedGoogle Scholar
  • 3 Hellem A. J. 1960; The Adhesiveness of Human Blood Platelets in vitro. Scandinavian Journal of Clinical and Laboratory Investigation 12 (Suppl. 51) 1.
    CrossrefPubMedGoogle Scholar
  • 4 Marmur A, Gill W. N, Ruckenstein E. 1976; Kinetics of Cell Deposition Under the Action of an External Field.. Bull. Math. Biology 38.
    PubMedGoogle Scholar
  • 5 Mason R. G, Gilkey J. M. 1971; A Simple Test for Quantitation of Platelet Adhesion to Glass. Studies in Bleeder and Non-bleeder Subjects, Thrombosis et Diathesis Haemorrhagica 25: 21.
    PubMedGoogle Scholar
  • 6 Roberts H. R, Kroncke Jr. F. G. 1971. In: Brinkhouse K. M, Shermer R. W, Mostofi F. K. (Eds.) Tests of Platelet Activity : Application to Clinical Diagnosis in “The Platelet”. Williams and Wilkins; Baltimore.:
    Google Scholar
  • 7 Robertson C. R, Chang H. N. 1974; Platelet Retention in Columns Packed with Glass Beads. Annals of Biomedicine 2: 361.
    PubMedGoogle Scholar
  • 8 Ruckenstein E, Prieve D. C. 1975; Dynamics of Cell Deposition on Surfaces. Journal of Theoretical Biology 51: 429.
    CrossrefPubMedGoogle Scholar
  • 9 Salzman E. W. 1963; Measurement of Platelet Adhesiveness - A Simple in vitro Technique Demonstrating an Abnormality in von Willebrand’s Disease. Journal of Laboratory and Clinical Medicine 62: 724.
    PubMedGoogle Scholar
  • 10 Weiss H. J. 1975; Platelet Physiology and Abnormalities of Platelet Function. The New England Journal of Medicine 293 Part I: 531 Part II: 580.
    CrossrefPubMedGoogle Scholar
  • 11 Zucker M. B, Vroman L. 1969; Platelet Adhesion Induced by Fibrinogen Adsorbed onto Glass. Proceedings of the Society for Experimental Biology and Medicine 131: 318.
    PubMedGoogle Scholar