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DOI: 10.1055/s-0038-1648049
Sedimentation and Adhesion of Platelets onto a Horizontal Glass Surface
Publikationsverlauf
Received
10. Januar 1976
Accepted
18. Mai 1976
Publikationsdatum:
03. Juli 2018 (online)
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Summary
An experimental method for measuring platelet adhesion to a glass surface and platelet sedimentation rate is described. Anticoagulated platelet-rich plasma is placed on a horizontal glass slide for various contact periods. The number of platelets adhering to the slide per unit area is recorded as a function of time. The experimental results are used to verify theoretical predictions which account for the effect of the slow sedimentation rate of the platelets and for their escape over the potential barrier between them and the solid surface. By a least-squares fit of the theoretical equation to the experimental data, both the platelet adhesiveness, in terms of P the probability to overcome the potential barrier, and the platelet sedimentation rate V are evaluated. A range of values of P and V for healthy humans is presented.
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References
- 1 Curtis A. S. G. 1973; In: Butler and Noble (Eds.) Cell Adhesion. Progress in Biophysics and Molecular Biology 27 Pergamon Press 315.
- 2 George J. N, Weed R. I, Reed C. F. 1971; Adhesion of Human Erythrocytes to Glass: The Nature of the Interaction and the Effect of Serum and Plasma. Journal of Cell. Physiology 77: 51.
- 3 Hellem A. J. 1960; The Adhesiveness of Human Blood Platelets in vitro. Scandinavian Journal of Clinical and Laboratory Investigation 12 (Suppl. 51) 1.
- 4 Marmur A, Gill W. N, Ruckenstein E. 1976; Kinetics of Cell Deposition Under the Action of an External Field.. Bull. Math. Biology 38.
- 5 Mason R. G, Gilkey J. M. 1971; A Simple Test for Quantitation of Platelet Adhesion to Glass. Studies in Bleeder and Non-bleeder Subjects, Thrombosis et Diathesis Haemorrhagica 25: 21.
- 6 Roberts H. R, Kroncke Jr. F. G. 1971. In: Brinkhouse K. M, Shermer R. W, Mostofi F. K. (Eds.) Tests of Platelet Activity : Application to Clinical Diagnosis in “The Platelet”. Williams and Wilkins; Baltimore.:
- 7 Robertson C. R, Chang H. N. 1974; Platelet Retention in Columns Packed with Glass Beads. Annals of Biomedicine 2: 361.
- 8 Ruckenstein E, Prieve D. C. 1975; Dynamics of Cell Deposition on Surfaces. Journal of Theoretical Biology 51: 429.
- 9 Salzman E. W. 1963; Measurement of Platelet Adhesiveness - A Simple in vitro Technique Demonstrating an Abnormality in von Willebrand’s Disease. Journal of Laboratory and Clinical Medicine 62: 724.
- 10 Weiss H. J. 1975; Platelet Physiology and Abnormalities of Platelet Function. The New England Journal of Medicine 293 Part I: 531 Part II: 580.
- 11 Zucker M. B, Vroman L. 1969; Platelet Adhesion Induced by Fibrinogen Adsorbed onto Glass. Proceedings of the Society for Experimental Biology and Medicine 131: 318.