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Thromb Haemost 1973; 29(03): 701-711
DOI: 10.1055/s-0038-1648113
Original Article
Schattauer GmbH

Lipid Infusions in Man

Blood Platelet Uptake of Lipid and Effects on Platelet Functions
K. A Grøttum
1   Section of Haematology (Chief, Professor P. F. Hjort), Medical Department A (Chief, Professor E. Gjone): Institute for Thrombosis Research (Chief, Professor H. Stormorken), Rikshospitalet, Oslo, Norway
,
A Nordøy
1   Section of Haematology (Chief, Professor P. F. Hjort), Medical Department A (Chief, Professor E. Gjone): Institute for Thrombosis Research (Chief, Professor H. Stormorken), Rikshospitalet, Oslo, Norway
,
A Hellem
1   Section of Haematology (Chief, Professor P. F. Hjort), Medical Department A (Chief, Professor E. Gjone): Institute for Thrombosis Research (Chief, Professor H. Stormorken), Rikshospitalet, Oslo, Norway
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Publikationsdatum:
24. Juli 2018 (online)

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Summary

The soybean lipid emulsion Intralipid was infused during two hours in 13 volunteer individuals. Biochemically, lipid infusions increased the platelet content of triglycerides, but did not cause significant platelet aggregation, and the platelet counts and survival time were unchanged. However, the lipid infusions reduced platelet and erythrocyte electrophoric mobility, reduced the availability of platelet factor 3 by ADP or kaolin and reduced platelet adhesiveness. A coating of parts of the platelet surface by lipid particles or some other changes in the platelet membrane could explain the effects on platelet electrophoretic mobility, platelet factor 3, and adhesiveness.

 

  • References

  • 1 Abrahamsen A. F. 1968; A modification of the technique for Cr51-labelling of blood platelets giving increased circulating platelet radioactivity. Scandinavian Journal of Haematology 05: 53.
    PubMedSuche in Google Scholar
  • 2 Barlett G. R. 1959; Phosphorus assay in column chromatography. Journal of Biological Chemistry 234: 466.
    PubMedSuche in Google Scholar
  • 3 Born G. V. R. 1962; Quantitative investigations into aggregation of blood platelets. Journal of Physiology (Lond) 162: 67.
    PubMedSuche in Google Scholar
  • 4 Brecher G. M, Schneideman M, Cronkite E. P. 1953; The reproducibility and constancy of the platelet count. American Journal of Clinical Pathology 23: 15.
    CrossrefPubMedSuche in Google Scholar
  • 5 Cohen P, Braunwald J, Gardner F. H. 1965; Destruction of canine and rabbit platelets following intravenous administration of carbon particles or endotoxin. Journal of Laboratory and Clinical Medicine 66: 263.
    PubMedSuche in Google Scholar
  • 6 Cronberg S, Nilsson I. M. 1967; Coagulation studies after administration of a fat emulsion, Intralipid. Thrombosis et Diathesis Haemorrhagica 18: 664.
    PubMedSuche in Google Scholar
  • 7 Dudgeon L. S, Goadby H. K. 1931; The examination of the tissues and some observations on the blood platelets of rabbits at intervals of five minutes, and later, after intravenous inoculations of staphyloccoeus aureus and Indian ink. Journal of Hygiene 03: 247.
    PubMedSuche in Google Scholar
  • 8 GRøTTUM K. A. 1968; Influence of aggregating agents on electrophoretic mobility of blood platelets from healthy individuals and from patients with cardiovascular diseases. Lancet I: 1406.
    PubMedSuche in Google Scholar
  • 9 Grøtttjm K. A, Solum N. O. 1969; Congenital thrombocytopenia with giant platelets: a defect in the platelet membrane. British Journal of Haematology 16: 277.
    CrossrefPubMedSuche in Google Scholar
  • 10 Grøttum K. A, Jörgensen L, Jeremic M. 1972; Decrease in platelet surface charge during phagocytosis of polystyrene latex particles, or thorium dioxide. Scandinavian Journal of Haematology 09: 83.
    PubMedSuche in Google Scholar
  • 11 Haslam R. J. 1964; Role of adenosine diphosphate in the aggregation of human blood platelets by thrombin and by fatty acids. Nature (Lond) 202: 765.
    CrossrefPubMedSuche in Google Scholar
  • 12 Hellem A. 1960 The adhesiveness of human blood platelets in vitro. Scandinavian Journal of Clinical and Laboratory Investigation. 12. suppl. 51..
    PubMedSuche in Google Scholar
  • 13 Hellem A. 1970; Platelet adhesiveness in von Willebrand’s disease. A study of a new modification of the glass bead filter method. Scandinavian Journal of Haematology 07: 374.
    PubMedSuche in Google Scholar
  • 14 Hjort P. F, Stoemoekbn H. 1957 A study of the in vitro and in vivo effects of a synthetic heparin-like anticoagulant: Dextran sulphate. Scandinavian Journal of Clinical and Laboratory Investigation. suppl. 29..
    PubMedSuche in Google Scholar
  • 15 Hovig T, Geøttum K. A. Lipid infusions in man: Ultrastructural studies on blood platelet uptake of fat particles. Thrombosis et Diathesis Haemorrhagica. 29.
    PubMed
  • 16 Håkansson I. 1966; Phy sico - chemical changes in artificial fat emulsions during storage. Acta chemica Scandinavica 20: 2267.
    CrossrefPubMedSuche in Google Scholar
  • 17 Laurell S. 1966; A method of routine determination of plasma triglycerides. Scandinavian Journal of Clinical and Laboratory Investigation 18: 668.
    CrossrefPubMedSuche in Google Scholar
  • 18 Lowry H. O, Rosebeough N. J, Lewis Farr A, Randall R. J. 1951; Protein measurements with the folin phenol reagent. Journal of Biological Chemistry 193: 265.
    PubMedSuche in Google Scholar
  • 19 Marinetti G. V. 1962; Chromatographic separation, identification and analysis of phospholipids. Journal of Lipid Research 03: 1.
    PubMedSuche in Google Scholar
  • 20 McGehee W. G, Hjort P. F, Rapaport S. I. 1966; Effects of thorium dioxide upon blood clotting and platelets. II. In vivo studies in rabbits. Thrombosis et Diathesis Haemorrhagica 16: 354.
    PubMedSuche in Google Scholar
  • 21 Miller G. L. 1959; Protein determination of large number of samples. Analytical Biochemistry 964: 31.
    PubMedSuche in Google Scholar
  • 22 Morgenstern E, Pfleiderer Th, Zebisch P, Weber E. 1969; Über die Wirkung von Lipidemulsionen auf Blutplättchen. II. Licht- und elektronenmikroskopische Studien. Thrombosis et Diathesis Haemorrhagica 22: 525.
    PubMedSuche in Google Scholar
  • 23 Mustard J. F, Packham M. A. 1970; Factors influencing platelet function: Adhesion, release and aggregation. Pharmacological Reviews 22: 97.
    PubMedSuche in Google Scholar
  • 24 Mustard J. F, Packham M. A. 1968; Platelet phagocytosis. Series Haematologica 01: 168.
    PubMedSuche in Google Scholar
  • 25 Nordøy A, Lund S. 1968; Platelet factor 3 activity, platelet phospholipids and their fatty acid and aldehyde pattern in normal male subjects. Scandinavian Journal of Clinical and Laboratory Investigation 22: 328.
    CrossrefPubMedSuche in Google Scholar
  • 26 Pfleiderer Th, Morgenstern E, Weber E. 1969; Über die Wirkung von Lipidemulsionen auf Blutplättchen. I. Zunahme der Plättchenaggregation unter dem Einfluß verschiedener Lipidemulsionen in vitro. Thrombosis et Diathesis Haemorrhagica 22: 513.
    PubMedSuche in Google Scholar
  • 27 Saba Th. M. 1970; Physiology and physiopathology of the reticulo-endothelial system. Archives of Internal Medicine 126: 1031.
    CrossrefPubMedSuche in Google Scholar
  • 28 Sachtleben P. 1965. The influences of antibodies on the electrophoretic mobility of red blood cells. In: Ambrose E. J. (ed.): Gell Electrophoresis. J. & A. Churchill Ltd; London: 100.
    Suche in Google Scholar
  • 29 Schulz H. 1968. Thrombocyten und Thrombose im elektronenmikroskopischen Bild. Springer - Verlag; Berlin.:
    Suche in Google Scholar
  • 30 Shafrir E, Biale Y. 1970; Effect of experimental hypertriglyceridaemia on tissue and serum lipoprotein lipase activity. European Journal of Clinical Investigation 01: 19.
    CrossrefPubMedSuche in Google Scholar
  • 31 Singer J. M, Adlersberg L, Hoenig E. M, Ende E. M, Ende E, Tchorsch Y. 1969; Radiolabeled latex particles in the investigation of phagocytosis in vivo: Clearance curves and histological observations. Journal of the Reticuloendothelial Society 06: 561.
    PubMedSuche in Google Scholar
  • 32 Tait J, Elvigde A. R. 1926; Effect upon platelets and on blood coagulation of injecting foreign particles into the blood stream. Journal of Physiology 62: 129.
    CrossrefPubMedSuche in Google Scholar
  • 33 Drugs Today’s. 1970 Solutions for intravenous feeding. British Medical Journal. I. 352.
    PubMedSuche in Google Scholar
  • 34 Van Aken W. G, Goote Th. M, Vreeken J. 1968; Platelet aggregation: an intermediary mechanism in carbon clearance. Scandinavian J ournal of Haematology 05: 333.
    PubMedSuche in Google Scholar
  • 35 Zbinden G. 1967; Laurie acid-induced thrombocytopenia and thrombosis in rabbits. Thrombosis et Diathesis Haemorrhagica 18: 57.
    PubMedSuche in Google Scholar