Thromb Haemost 1991; 65(04): 335-338
DOI: 10.1055/s-0038-1648147
Original Article
Schattauer GmbH Stuttgart

Immunocytochemical Study on Endothelial Markers with the Cerebral Vessel from a Patient with Familial Moyamoya Disease

Fusako Usuki
1   The Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan
3   The Sakimoto Hospital, Kagoshima, Japan
,
Haruo Ohtani
2   The Department of Pathology, Tohoku University School of Medicine, Sendai, Japan
,
Toshihide Okadome
1   The Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan
,
Koichi Shinmyozu
1   The Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan
,
Ikuro Maruyama
1   The Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan
,
Tetsuro Sakimoto
3   The Sakimoto Hospital, Kagoshima, Japan
,
Masayuki Atsuji
4   The Atsuli Hospital of Neurosurgery, Kagoshima, Japan
,
Mitsuhiro Osame
1   The Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received: 25. Juli 1990

Accepted after revision 23. November 1990

Publikationsdatum:
02. Juli 2018 (online)

Summary

The immunocytochemical study of thrombomodulin (TM), a newly recognued anticoagulant endothelial surface protein, was performed with a surgical specimen of a superficial temporalartery (STA) obtained from a 29-year-old woman with familial moyamoya disease. The staining of TM showed positive immunoreactivity in smaller vessels in the surrounding connective tissue of the specimen, whereas negative in STA. Immunoelectronmicroscopically the luminal plasma membrane of endothelial cells was positive for TM. These staining pattern was the same as that in controls. She concurrently suffered from von Willebrand disease type I, and she had two cerebral hemorrhagic attacks. A quantitative defect of the von Willebrand factor in the endothelium was demonstrated immunocytochemically.

 
  • References

  • 1 Suzuki J, Thkaku A. cerebrovascular “moyamoya” disease. Arch Neurol 1969; 20: 288-299
  • 2 Esmon NL, Owen WG, Esmon CT. Isolation of a membrane bound cofactor for thrombin-catalyzed activation of protein C. J Biol Chem 1982; 257: 859-864
  • 3 Marlar RA, Kleiss AJ, Griffin JH. Mechanism of action of human activated protein C, a thrombin-dependent anticoagulant ervyme. Blood 1982; 59: 1067-1072
  • 4 Suzuki K, Stenflo J, Dahlback B, Teodorsson G. Inactivation of human coagulation factor V by activated protein C. J Biol Chem 1983; 258: 1914-1920
  • 5 Salem HH, Maruyama I, Ishii H, Majerus pw. Isolation and characterization of thrombomodulin from human placenta. J Biol Chem 1984; 259: 12246-12251
  • 6 Suzuki K, Kusumoto H, Deyashiki y, Nishioka J, Maruyama I, Zushi M, Kagahara S, Honda G, yamamoto S, Horiguchi S. Structure and expression of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for protein C. EMBO J 1987; 5: 1891-1897
  • 7 Maruyama I, Bell E, Majerus PW. Thrombomodulin is found on endothelium of arteries, veins, capillaries, and lymphatics, and on syncytiotrophoblast of human placenta. J Cell Biol 1985; 101: 363-371
  • 8 Maruyama I. Functional and morphologic alterations of endothelium induced by thrombin. Jpn J Arteriosclerosis 1988; 16: 911-915
  • 9 Kumada I, Dittman WA, Majerus PW. A role for thrombomodulin in the pathogenesis of thrombin-induced thromboembolism in mice. Blood 1987; 71: 72843
  • 10 Ohtani ff, Sasano N. Characterization of microvasculature in the stroma of human colorectal carcinoma: An immunoelectron microscopic study on factor VIIVvon Willebrand factor. J Electron Microsc 1987; 36: 204-212
  • 11 Ishii H, Salem HH, Bell CE, Laposata EA, Majerus pw. Thrombomodulin, an endothelial anticoagulant protein, is absent from the human brain. Blood 1986; 67: 362-365
  • 12 Wen D, Dittman WA, Ye RD, Deaven LL, Majerus PW, Sadler JE. Human thrombomodulin: complete cDNA sequence and chromosome localization of the gene. Biochemistry 1987; 26: 4350-4357
  • 13 Ohtani H, Maruyama I, Yonezawa S. Ultrastructural immunolocalization of thrombomodulin in human placenta with microwave fixation. Acta Histochem 1989; 22: 393-395
  • 14 Jaffe EA, Hoyer TW, Nachman RL. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin lnvest 1973; 52: 2757-2764
  • 15 Sporn LA, Chavin SI, Marder VJ, Wagner DD. Biosynthesis of von Willebrand protein by human megakaryocytes. J Clin Invest 1985; 76: 1102-1106
  • 16 Meyer D, Baumgartner HR. Role of von Willebrand factor in platelet adhesion to the subendothelium. Br J Haematol 1983; 54: 1-9
  • 17 Ruggeri ZM, Zimmerman TS. Variant von Willebrand’s disease. Characteraation of two subtypes by analysis of multimeric composition of factor VIII/von Willebrand factor in plasma and platelets. J Clin Invest 1980 65. 1318-1325