Download PDF
Thromb Haemost 1991; 65(05): 478-482
DOI: 10.1055/s-0038-1648175
Original Article
Schattauer GmbH Stuttgart

No Evidence of HIV-1 Infection in Seronegative Hemophiliacs and in Seronegative Partners of Seropositive Hemophiliacs through Polymerase Chain Reaction (PCR) and Anti-NEF Serology

Françoise Ferrer-Le-Coeur
The Institut National de Transfusion Sanguine, Paris, France
,
Martine Mariotti
The Institut National de Transfusion Sanguine, Paris, France
,
Philippe Hivert
The Institut National de Transfusion Sanguine, Paris, France
,
Eric Pascal Satre
The Institut National de Transfusion Sanguine, Paris, France
,
Françoise Bouchardeau
The Institut National de Transfusion Sanguine, Paris, France
,
Anne-Marie Couroucé
The Institut National de Transfusion Sanguine, Paris, France
,
Philippe Rouger
The Institut National de Transfusion Sanguine, Paris, France
,
Jean-Jacques Lefrère
The Institut National de Transfusion Sanguine, Paris, France
› Author Affiliations
Further Information

Publication History

Received 21 September 1990

Accepted after revision 14 December 1990

Publication Date:
24 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

We prospectively studied a well-characterized cohort including 60 seronegative hemophiliacs or von Willebrand’s disease patients, 6 seronegative female sexual partners of seropositive hemophiliacs, 59 seropositive hemophiliacs or von Willebrand’s disease patients and 2 seropositive partners of seropositive hemophiliacs (used as positive controls), and 117 seronegative low risk individuals (used as negative controls). PCR assay, performed in peripheral blood mononuclear cells using three primer pairs in the gag, pol, LTR regions, showed no positive results in the 60 seronegative patients, in the 6 seronegative partners of seropositive patients and in the 117 seronegative low risk individuals, while PCR was positive with at least one primer pair in 53 (87%) of 61 seropositive patients. Anti-nef serology (Westem-blot) was negative in seronegative patients, in seronegative partners of seropositive patients and positive in 58% out of the seropositive individuals. These results strongly suggest an absence of HIV-1 infection in individuals with a lastingly negative HIV serology.

 

  • References

  • 1 Allain JP. Prevalence of HTLV-III/LAV antibodies in patients with hemophilia and in their sexual partners in France. N Engl J Med 1986; 315: 517-518
    CrossrefPubMedGoogle Scholar
  • 2 Smiley ML, White GC, Becherer P. et al Transmission of human immunodeficiency virus to sexual partners of hemophiliacs. Am J Hematol 1988; 28: 27-32
    CrossrefPubMedGoogle Scholar
  • 3 Kreiss JK, Kitchen LW, Prince HE. et al Antibody to human T-lymphotropic virus type III in wives of hemophiliacs: evidence for heterosexual transmission. Ann Intern Med 1985; 102: 623-626
    CrossrefPubMedGoogle Scholar
  • 4 Kim HC, Raska K, Clemow L. et al Human immunodeficiency virus infection in sexually active wives of infected hemophilic men. Am J Med 1988; 85: 472-476
    CrossrefPubMedGoogle Scholar
  • 5 Ranki AM, Valle SL, Krohn M. et al Long latency precedes overt seroconversion in sexually transmitted human immunodeficiency virus infection. Lancet 1987; 2: 5889-5893
    PubMedGoogle Scholar
  • 6 Imagawa DT, Lee MH, Wolinski SM. et al Human immunodeficiency virus type 1 infection in homosexual men who remain seronegative for prolonged periods N Engl J Med 1989; 320: 1458-1462
    CrossrefPubMed
  • 7 Saiki RK, Scharf S, Faloona F. et al Enzymatic amplification of betaglobin genomic sequences and restriction site analysis for diagnosis of sickle-cell anemia. Science 1985; 230: 1350-1354
    CrossrefPubMedGoogle Scholar
  • 8 Kwok S, Mack DH, Mullis KB. et al Identification of human imunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection. J Virol 1987; 61: 690-694
    PubMedGoogle Scholar
  • 9 Ou CY, Kwok S, Mitchell SW. et al DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science 1988; 239: 295-297
    CrossrefPubMedGoogle Scholar
  • 10 Loche M, Bach B. Identification of HIV-infected seronegative individuals by a direct diagnostic test based on hybridisation to amplified viral DNA. Lancet 1988; 2: 418-421
    PubMedGoogle Scholar
  • 11 Wolinski SM, Rinaldo CR, Kwok S. et al Human immunodeficiency virus type 1 (HIV-1) infection. A median of 18 months before a diagnostic Western blot. Ann Intern Med 1989; 111: 961-972
    CrossrefPubMedGoogle Scholar
  • 12 Ameisen JC, Guy B, Lecoq JP. et al Persistent antibody response to the HIV-1 negative regulatory factor in HIV-1 infected seronegative persons. N Engl J Med 1989; 320: 241-242
    PubMedGoogle Scholar
  • 13 Soriano V, Hewlett I, Tor J, Clotet B, Epstein J, Foz M. Silent HIV infection in heterosexual partners of seropositive drug abusers in Sapin. Lancet 1990; i: 860
    PubMedGoogle Scholar
  • 14 Hewlett IK, Laurian Y, Epstein J, Allain JP. Analysis by PCR of transmission of HIV from infected hemophiliacs to their sex partners and children. Vth International Conference on AIDS, 1989 (abstract MAO 33)
    PubMedGoogle Scholar
  • 15 Eyster ME, Goedert J, Kumar R. Failure of the polymerase chain reaction (PCR) to detect viral HIV-1 DNA by amplification of a gag segment in hemophiliacs with HIV antibodies. Vth International Conference on AIDS, 1989 (abstract TBP 82)
    PubMedGoogle Scholar
  • 16 Moncany MLJ, Berthier A, Ferries S, Montagnier L. Early detection of HIV infection by PCR before seroconversion in multitransfused seronegative haemophiliacs and sexual partners. Sixth International Conference on AIDS, San Francisco, 1990 (Th A 14)
    PubMedGoogle Scholar
  • 17 Jason J, Ou CY, Moore JL. et al Prevalence of human immunodeficiency virus type 1 DNA in hemophilic men and their sex partners. J Infect Dis 1989; 160 (05) 789-794
    CrossrefPubMedGoogle Scholar
  • 18 De Ronde A, Reiss P, Dekker J. et al Seroconversion to HIV-1 negative regulation factor. Lancet 1988; ii: 574
    PubMedGoogle Scholar
  • 19 Centers for Disease Control: Classification system for human T-lymphotropic virus type III/lymphadenopathy-associated virus infections. MMWR 1986; 35: 334-339
    PubMedGoogle Scholar
  • 20 Wain-Hobson S, Sonigo P, Danos O, Cole S, Alizon M. Nucleotide sequence of the Aids virus. Cell 1985; 40: 9-17
    CrossrefPubMedGoogle Scholar
  • 21 Ranki A, Jarvinen K, Valle SL, Nurmilaakso P, Krohn K. Antibodies to recombinant HIV-1 nef protein detected in HIV-1 infection as well as in non-risk individuals. J AIDS 1989; 3: 348-355
    PubMedGoogle Scholar
  • 22 Taylor GR. HIV detection by DNA amplification. J Clin Pathol 1988; 41: 1244-1246
    CrossrefPubMedGoogle Scholar
  • 23 Markoulatos P, Ferris S, Karagiorga-Logana M, Kordossi A, Montagnier L, Moncany MLJ. Study of HIV infection by PCR in seronegative multitransfused beta-thalassemiac patients. Sixth International Conference on AIDS; San Francisco: 1990. (FA 353)
    Google Scholar
  • 24 Lifson AR, Stanley M, Pane J. et al Detection of human immunodeficiency virus DNA using the polymerase chain reaction in a well-characterized group of homosexual and bisexual men. J Infect Dis 1990; 161: 436-439
    CrossrefPubMedGoogle Scholar
  • 25 Lefrere JJ, Mariotti M, Courouce AM, Rouger P, Salmon C, Vittecoq D. Polymerase chain reaction testing of HIV-1 seronegative at-risk individuals. Lancet 1990; i: 1400-1401
    PubMedGoogle Scholar
  • 26 Mariotti M, Lefrere JJ, Noel B. et al DNA amplification of HIV-1 in seropositive individuals and in seronegative at-risk individuals. AIDS 1990; 4: 633-637
    CrossrefPubMedGoogle Scholar
  • 27 Lefrère JJ, De Montalembert M, Mariotti M, Girot R, Salmon C, Rouger P, Rey J. Absence of DNA sequences in seronegative poly transfused thalassemic patients. Vox Sang, in press
    PubMedGoogle Scholar
  • 28 Shoebridge GI, Gatenby PA, Nightingale BN. et al Polymerase chain reaction testing of HIV-1 seronegative at-risk individuals. Lancet 1990; ii: 180-181
    PubMedGoogle Scholar
  • 29 Hackson JB, Kwok SY, Hopsicker JS. et al Absence of HIV-1 infection in antibody-negative sexual partners of HIV-1 infected hemophiliacs. Transfusion 1989; 29 (03) 265-267
    CrossrefPubMedGoogle Scholar
  • 30 Lefrere JJ, Richard D, Courouce AM. et al Risk factors of HIV seropositive subjects detected through blood donation in France. Transfusion 1989; 29: 84-86
    PubMedGoogle Scholar
  • 31 Horsburgh C, Ou CY, Jason J. et al Duration of human immunodeficiency virus infection before detection of antibody. Lancet 1989; ii: 637-641
    PubMedGoogle Scholar
  • 32 Van Der Meer J, Daenen S, Van Imhoff GW, de Wolf JT, Halie MR. Absence of seroconversion for HTLV-III in haemophiliacs intensively treated with heat treated factor VIII concentrate. Br Med J 1986; 292: 1049
    CrossrefPubMedGoogle Scholar
  • 33 Jackson JB, Balfour HH. Hemophiliacs with HIV seroconversion associated with heat-treated products. Are they actively infected?. JAMA 1989; 261: 1275
    PubMedGoogle Scholar
  • 34 Gluckmann JC, Fretz-Foucault C, Rouzioux C. et al Lack of anti-p27 nef antibody detection in HIV seronegative high risk people. AIDS 1989; 3 (12) 855
    PubMedGoogle Scholar
  • 35 Reiss P, De Ronde A, Lange JMA. et al Antibody response to the viral negative factor (nef) in HIV-1 infection: a correlate of levels of HIV-1 expression. AIDS 1989; 3: 227-233
    CrossrefPubMedGoogle Scholar