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Thromb Haemost 1991; 65(05): 545-548
DOI: 10.1055/s-0038-1648187
Original Article
Schattauer GmbH Stuttgart

Cellular Localization of Activated Factor X by Xa-Specific Probes

Leo R Zacharski
1   The VA Medical Center, White River Junction, Vermont, U.S.A.
2   The Department of Medicine, Dartmouth Medical School, Pennsylvania, U.S.A.
,
Christopher Dunwiddie
3   The Department of Pharmacology, Merck, Sharp and Dohme Research Laboratories, West Point, Pennsylvania, U.S.A.
,
Elka M Nutt
3   The Department of Pharmacology, Merck, Sharp and Dohme Research Laboratories, West Point, Pennsylvania, U.S.A.
,
Jane Hunt
1   The VA Medical Center, White River Junction, Vermont, U.S.A.
,
Vincent A Memoli
4   The Department of Pathology, Dartmouth Medical School, Pennsylvania, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 05 November 1990

Accepted after revision 16 January 1991

Publication Date:
24 July 2018 (online)

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Summary

A probe, recombinant antistasin, that reacts specifically with the activated form of factor X (Xa) was used in immunohistochemical procedures to detect cellular sites of Xa generation within intact tissues. Factor Xa was detected on tumor cells in small cell carcinoma of the lung, renal cell carcinoma, and malignant melanoma. Tumor-associated macrophages (but not tumor cells) expressed Xa in adenocarcinoma and squamous cell carcinoma of the lung, and Hodgkin’s disease. Factor Xa in these locations corresponded to evidence reported previously for an intact coagulation pathway and thrombin formation associated with these tumor cells and macrophages. By contrast, only rare connective tissue cells stained for Xa in breast and colon cancer, tumor types shown previously to lack an intratumoral coagulation pathway and thrombin generation, and in normal liver, lung, breast, kidney, and placental tissues. Hepatocytes did not stain. These results suggest that such probes may be useful for studying the activation state of cell-associated factor X in situ within intact tissues.

 

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