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Thromb Haemost 1991; 65(05): 581-587
DOI: 10.1055/s-0038-1648194
Original Article
Schattauer GmbH Stuttgart

Platelets Adhere to Sulfatides by von Willebrand Factor Dependent and Independent Mechanisms

Richard E Data
The Hematology Service, Clinical Pathology Department, Clinical Center, Bethesda, MD, U.S.A.
,
Sybil B Williams
The Hematology Service, Clinical Pathology Department, Clinical Center, Bethesda, MD, U.S.A.
,
David D Roberts
*   The Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A.
,
Harvey R Gralnick
The Hematology Service, Clinical Pathology Department, Clinical Center, Bethesda, MD, U.S.A.
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Publikationsverlauf

Received 02. Oktober 1990

Accepted after revision 09. Januar 1991

Publikationsdatum:
24. Juli 2018 (online)

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Summary

Unstimulated human platelets from normal volunteers adhere to sulfatides (galactosylceramide-I3-sulfate) as single cells but do not adhere appreciably to other lipids including gangliosides, neutral glycolipids, phospholipids or cholesterol-3-SO4. Platelet adhesion to sulfatide is saturable and dose-dependent, reaches maximal levels in 90 to 120 min, and is not divalent cation-dependent. Because sulfatides bind von Willebrand factor (vWf) with specificity and high affinity and platelet adhesion to structurally related sulfated glycolipids is approximately proportionate to their ability to bind vWf, we examined whether vWf mediates platelet adhesion to sulfatides. Platelets from a patient with severe Type I von Willebrand’s disease adhere poorly to sulfatides. However, adhesion to levels seen with normal platelets is restored by the addition of vWf. Adhesion of normal platelets can be partially inhibited by a monospecific antibody to vWf. Normal platelet adhesion to sulfatides, however, is not increased following preincubation with vWf. Both vWf binding and platelet adhesion to sulfatides can be inhibited by the sulfated polysaccharide dextran sulfate at low concentration, fucoidan at high concentrations, but not by heparin, fibrinogen, fibronectin, or the synthetic peptides Gly-Arg-Gly-Asp-Ser-Pro or Gly-Arg-Gly-Glu-Ser-Pro. Thus, adhesion to sulfatides appears to be of two types; vWf dependent (50-75%) and vWf independent (25-50%).

 

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