Hirudin C-Terminal Fragments lnhibit Thrombin Induced Neutrophil Chemotaxis

Judith K Rowand; Philip Marucha; Lawrence J Berliner

doi:10.1055/s-0038-1648433

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1992; 67(03): 289-291
DOI: 10.1055/s-0038-1648433

Rapid Communications

Schattauer GmbH Stuttgart

Hirudin C-Terminal Fragments lnhibit Thrombin Induced Neutrophil Chemotaxis

Judith K Rowand

¹Department of Chemistry, The Ohio State University, Columbus, Ohio, USA

,

Philip Marucha

²College of Dentistry, The Ohio State University, Columbus, Ohio, USA

,

Lawrence J Berliner

¹Department of Chemistry, The Ohio State University, Columbus, Ohio, USA

› Institutsangaben

Abstract

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Summary

Since native hirudin blocks the thrombin induced chemotaxis response of neutrophils, we examined whether hirudin C-terminal peptides were also capable of this inhibition. The studies showed that thrombin induced human neutrophil chemotaxis was effectively blocked by the C-terminal hirudin peptide analogs, Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-Gin (12-mer[54-65]) and Thr-Pro-Lys-Pro-Gln-Ser-His-Asn-Asp-Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-Gln (21-mer[45-65]). Furthermore, neither peptide had an effect on formyl-L-methionyl-L-leucyl-L-phenylalanine induced chemotaxis. The results suggest that binding of the hirudin C-terminal peptides block the thrombin chemotactic domain.

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Referenzen

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