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Thromb Haemost 1992; 67(04): 434-439
DOI: 10.1055/s-0038-1648466
Original Articles
Schattauer GmbH Stuttgart

The Effect of Suramin on Laboratory Tests of Coagulation

McDonald K Horne III
*   The Hematology Section, Clinical Pathology Department, Warren Grant Magnuson Clinical Center, and the Pharmacology Branch, Maryland, USA
,
Olga J Wilson
*   The Hematology Section, Clinical Pathology Department, Warren Grant Magnuson Clinical Center, and the Pharmacology Branch, Maryland, USA
,
Michael Cooper
**   The Hematology Section, Clinical Pathology Department, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
,
Harvey R Gralnick
*   The Hematology Section, Clinical Pathology Department, Warren Grant Magnuson Clinical Center, and the Pharmacology Branch, Maryland, USA
,
Charles E Myers
**   The Hematology Section, Clinical Pathology Department, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
› Author Affiliations
Further Information

Publication History

Received 04 July 1991

Accepted after revision 22 October 1991

Publication Date:
03 July 2018 (online)

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Summary

The antitrypanosomal drug suramin, which has recently been under investigation as a cancer chemotherapeutic agent, has previously been found to induce heparin-like anticoagulants in treated patients. In the currently reported work suramin is shown to have an additional anticoagulant activity that is due to direct effects of the drug on procoagulant proteins. The studies were conducted with pooled normal plasma treated in vitro with suramin and with plasma samples obtained from patients who had received the drug intravenously for 2 weeks. It is demonstrated that in plasma suramin inhibits factors V, VIII, IX, X, XI, and XII, while thrombin, prothrombin, and factor VII are unaffected. The inhibition of factor V is virtually irreversible, although the effect of suramin on the other factors is readily reversed by dilution.

 

  • References

  • 1 La Rocca RV, Stein CA, Myers CE. Suramin: prototype of a new generation of antitumor compounds. Cancer Cells 1990; 2: 106-115
    PubMedGoogle Scholar
  • 2 Home MK, Stein CA, La Rocca RV, Myers CE. Circulating glycosaminoglycan anticoagulants associated with suramin treatment. Blood 1988; 71: 273-279
    PubMedGoogle Scholar
  • 3 Klecker RW, Collins JM. Quantification of suramin by reverse-phase ion-pairing high-performance liquid chromatography. J Liquid Chromat 1985; 8: 1685-1696
    PubMedGoogle Scholar
  • 4 Blumenkrantz N, Asboe-Hansen G. New method for quantitative determination of uronic acid. Anal Biochem 1973; 54: 484-489
    CrossrefPubMedGoogle Scholar
  • 5 von Clauss A. Gerinnungsphysiologische Schnellmethode zur Bestim-mung des Fibrinogens. Acta Haematol 1957; 17: 237-246
    CrossrefPubMedGoogle Scholar
  • 6 Triplett DA, Harms CS. Factor II, V, VII, or X assay. In: Procedures for the Coagulation Laboratory. Educational Products Division, American Society of Clinical Pathologists; Chicago, IL: 1981. pp 34-60
    Google Scholar
  • 7 Ellison N, Ominsky AJ, Wollman H. Is protamine a clinically important anticoagulant?. Anesthesiology 1971; 35: 621-629
    CrossrefPubMedGoogle Scholar
  • 8 Eisen V, Loveday C. Effects of suramin on complement, blood clotting, fibrinolysis and kinin formation. Br J Pharmacol 1973; 49: 678-687
    CrossrefPubMedGoogle Scholar
  • 9 Muller WE, Wollert U. Spectroscopic studies on the complex formation of suramin with bovine and human serum albumin. Biochim Biophys Acta 1976; 427: 465-480
    CrossrefPubMedGoogle Scholar
  • 10 Collins JM, Klecker RW, Yarchoan R, Lane HC, Fauci AS, Redfield RR, Broder S, Myers CE. Clinical pharmacokinetics of suramin in patients with HTLV-III/LVA infection. J Clin Pharmacol 1986; 26: 22-26
    CrossrefPubMedGoogle Scholar
  • 11 Vansterkenburg ELM, Wilting J, Janssen LHM. Influence of pH on the binding of suramin to human serum albumin. Biochem Pharmacol 1989; 38: 3029-3035
    CrossrefPubMedGoogle Scholar
  • 12 Town BW, Wills ED, Wilson EJ, Wormall A. Studies on suramin. 8. The action of the drug on enzymes and some other proteins. General considerations. Biochem J 1950; 47: 149-170
    CrossrefPubMedGoogle Scholar
  • 13 Mann KG, Jenny RJ, Krishnaswamy S. Cofactor proteins in the assembly and expression of blood clotting enzyme complexes. Annu Rev Biochem 1988; 57: 915-956
    CrossrefPubMedGoogle Scholar