Effects of Injected Antibody against the Platelet Glycoprotein llb/llla Complex on Monkey Platelet Fibrinogen

Masahiko Suzuki; Toshihiro Kawakatsu; Hirokazu Nagata; Kenjiro Hamamoto; Koji Iwata; Shigetoshi Ohga; Shosaku Nomura; Terutoshi Kokawa; Kojiro Yasunaga

doi:10.1055/s-0038-1648497

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1992; 67(05): 578-581
DOI: 10.1055/s-0038-1648497

Original Articles

Schattauer GmbH Stuttgart

Effects of Injected Antibody against the Platelet Glycoprotein llb/llla Complex on Monkey Platelet Fibrinogen

Masahiko Suzuki

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

,

Toshihiro Kawakatsu

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

,

Hirokazu Nagata

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

,

Kenjiro Hamamoto

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

,

Koji Iwata

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

,

Shigetoshi Ohga

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

,

Shosaku Nomura

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

,

Terutoshi Kokawa

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

,

Kojiro Yasunaga

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

› Author Affiliations

Abstract

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Summary

Four monkeys were injected for a 10-day period with the Fab fragment of a murine monoclonal antibody (NNKY 1-32) which inhibits the binding of fibrinogen to the platelet glycoprotein (GP) Ilb/IIIa complex. Platelet fibrinogen levels were assessed quantitatively by electroimmuno assay and qualitatively by immunoelectron microscopy.

The platelet fibrinogen level fell to 9.0 ± 2.8% of the control level after antibody administration. Immunoelectron microscopy showed that the injected antibody was localized on the inner surface of the platelet a-granule membrane.

Our findings suggest that the GP Ilb/IIIa complex can be internalized by a-granules and that it may mediate the endocytosis of plasma fibrinogen by platelets.

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References

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