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Thromb Haemost 1992; 67(05): 578-581
DOI: 10.1055/s-0038-1648497
Original Articles
Schattauer GmbH Stuttgart

Effects of Injected Antibody against the Platelet Glycoprotein llb/llla Complex on Monkey Platelet Fibrinogen

Masahiko Suzuki
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
,
Toshihiro Kawakatsu
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
,
Hirokazu Nagata
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
,
Kenjiro Hamamoto
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
,
Koji Iwata
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
,
Shigetoshi Ohga
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
,
Shosaku Nomura
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
,
Terutoshi Kokawa
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
,
Kojiro Yasunaga
The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
› Author Affiliations
Further Information

Publication History

Received 04 July 1991

Accepted after revision 03 December 1991

Publication Date:
03 July 2018 (online)

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Summary

Four monkeys were injected for a 10-day period with the Fab fragment of a murine monoclonal antibody (NNKY 1-32) which inhibits the binding of fibrinogen to the platelet glycoprotein (GP) Ilb/IIIa complex. Platelet fibrinogen levels were assessed quantitatively by electroimmuno assay and qualitatively by immunoelectron microscopy.

The platelet fibrinogen level fell to 9.0 ± 2.8% of the control level after antibody administration. Immunoelectron microscopy showed that the injected antibody was localized on the inner surface of the platelet a-granule membrane.

Our findings suggest that the GP Ilb/IIIa complex can be internalized by a-granules and that it may mediate the endocytosis of plasma fibrinogen by platelets.

 

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