Effects of Injected Antibody against the Platelet Glycoprotein llb/llla Complex on Monkey Platelet Fibrinogen

Masahiko Suzuki; Toshihiro Kawakatsu; Hirokazu Nagata; Kenjiro Hamamoto; Koji Iwata; Shigetoshi Ohga; Shosaku Nomura; Terutoshi Kokawa; Kojiro Yasunaga

doi:10.1055/s-0038-1648497

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1992; 67(05): 578-581
DOI: 10.1055/s-0038-1648497

Original Articles

Schattauer GmbH Stuttgart

Effects of Injected Antibody against the Platelet Glycoprotein llb/llla Complex on Monkey Platelet Fibrinogen

Authors

Masahiko Suzuki

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
Toshihiro Kawakatsu

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
Hirokazu Nagata

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
Kenjiro Hamamoto

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
Koji Iwata

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
Shigetoshi Ohga

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
Shosaku Nomura

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
Terutoshi Kokawa

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan
Kojiro Yasunaga

The First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

Abstract

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Summary

Four monkeys were injected for a 10-day period with the Fab fragment of a murine monoclonal antibody (NNKY 1-32) which inhibits the binding of fibrinogen to the platelet glycoprotein (GP) Ilb/IIIa complex. Platelet fibrinogen levels were assessed quantitatively by electroimmuno assay and qualitatively by immunoelectron microscopy.

The platelet fibrinogen level fell to 9.0 ± 2.8% of the control level after antibody administration. Immunoelectron microscopy showed that the injected antibody was localized on the inner surface of the platelet a-granule membrane.

Our findings suggest that the GP Ilb/IIIa complex can be internalized by a-granules and that it may mediate the endocytosis of plasma fibrinogen by platelets.

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References

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