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Thromb Haemost 1978; 40(01): 011-023
DOI: 10.1055/s-0038-1648540
Original Article
Schattauer GmbH Stuttgart

Ultrastructural Studies on the Surface Coat of Human Platelet Aggregated by Polylysine and Dextran[*]

Y Taketomi
The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, 734, Japan
,
A Kuramoto
The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, 734, Japan
› Author Affiliations
Further Information

Publication History

Received 23 August 1977

Accepted 06 February 1978

Publication Date:
12 July 2018 (online)

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Summary

Positively charged macromolecule, polylysine (mol. wt. 15,000; 23,000; 180,000) could induce the platelet aggregation in low concentration but high concentration was required in the case of neutral macromolecule, dextran (mol. wt. 40,000; 250,000; 2,000,000). The larger molecules of polylysine and dextran were more effective in inducing platelet aggregation. In the dextran-induced aggregation, positively charged Thorotrast particles on the cell surface did not decrease significantly. On the other hand, the surface membranes of platelets aggregated by polylysine were essentially devoid of bound particles. Heparin in hibited the poly lysine-induced platelet aggregation but not the dextran-induced aggregation. These findings suggested that polylysine induced aggregation more effectively than dextran by reducing the negative surface charge and giving stronger adsorption force on cell surface.

In platelet-rich plasma, polylysine elicited the release reaction of 14C-serotonin but dextran did not. Possible mechanism by which polylysine could elicit the release reaction is the formation of more tightly packed platelet aggregate than that by dextran in the presence of the low calcium ion concentration in citrated platelet-rich plasma. Average distance between plasma membranes of aggregated platelets, however, did not vary with the degrees of polymerization of these macromolecules.

* Presented in part at the 6th International Congress on Thrombosis and Haemostasis, Philadelphia, U.S.A., July 1, 1977.


 

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