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Thromb Haemost 1978; 40(01): 118-127
DOI: 10.1055/s-0038-1648641
Original Article
Schattauer GmbH Stuttgart

Effects of Heparin, Typhoid Vaccine and Thrombophlebitis on Factor X Metabolism in the Dog

Y Takeda
The Departrnent of Medicine, University of Colorado School of Medicine, Denver, Colorado 80220, U.S.A.
,
H Arai
The Departrnent of Medicine, University of Colorado School of Medicine, Denver, Colorado 80220, U.S.A.
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Further Information

Publication History

Received 28 April 1977

Accepted 28 March 1978

Publication Date:
12 July 2018 (online)

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Summary

Studies were made of the effects of heparin, typhoid vaccine and thrombophlebitis on factor X (F.X) metabolism in dogs, using 125-labelled F.X (125I-F.X) as a tracer. 10 healthy male dogs were used for control study and 5 male dogs were used for each of other studies. Plasma F.X concentration was measured by a solid-phase radioimmunoassay. In the control dogs, the plasma F.X concentration was 2.4 ± 0.2 (SD) mg per dl, the plasma F.X (x) was 1.2 ± 0.1(SD) mg per kg, the halflife of plasma 125I-F.X(t½) was 0.5 ± 0.01 (SD) days, the fractional catabolic rate (j3p) was 2.4 ± 0.1 (SD) per day and the catabolic flur (J3pX) (synthesis rate) was 2.9 ± 0.7 (SD) mg per kg per day. These results were not appreciably altered in heparinized dogs. However, a single intravenous injection of 2 ml typhoid vaccine caused a shortened t½ of 0.38 ± 0.01 (SD) days and a decrease of plasma F.X concentration to 1.3 ± 0.13 (SD) mg per dl. Studies in heparinized dogs showed similar effects of typhoid vaccine. Next, thrombophlebitis was produced by a previous method and its effects on F.X metabolism were studied. No detectable alterations of F.X metabolism were found. These results indicate that in health most F. X is directly catabolized without the formation of activated F.X (F.Xa), that the observed effects of typhoid vaccine are mostly due to an acceleration of the direct catabolism of F.X without F.Xa formation and finally that localized thrombophlebitis has no appreciable effects on F.X metabolism.

 

  • References

  • 1 Arai H, Takeda Y. 1977; Properties and radioimmunoassay of canine Factor X. Thrombosis Research 11: 57
    CrossrefPubMedSearch in Google Scholar
  • 2 Bajaj SP, Mann KG. 1973; Simultaneous purification of bovine prothrombin and Factor X. Journal of Biological Chemistry 248: 7729
    PubMedSearch in Google Scholar
  • 3 Biggs R, Denson KW E. 1963; The fate of prothrombin and Factors VIII, IX and X transfused to patients deficient in these Factors. British Journal of Haematology 9: 532
    CrossrefPubMedSearch in Google Scholar
  • 4 Davis BJ. 1964; Disc electrophoresis. II. Method and its application to human serum proteins. Annals of New York Academy of Sciences 121: 404
    PubMedSearch in Google Scholar
  • 5 Didisheim P, Loeb J, Blatrix C, Soulier JP. 1959; Preparation of a human plasma fraction rich in prothrombin, proconvertin, Stuart Factor, and PTC and a study of its activity and toxicity in rabbits and man. Journal of Laboratory and Clinical Medicine 53: 322
    PubMedSearch in Google Scholar
  • 6 Gladhaug A, Prydz H. 1970; Purification of the coagulation Factors VII and X from human serum. Some properties of Factor VII. Biochimica et biophysica acta 215: 105
    CrossrefPubMedSearch in Google Scholar
  • 7 Hogenauer E, Lechner K, Deutsch E. 1968; Isolation and characterization of blood clotting Factors VII and X. Thrombosis et Diathesis Haemorrhagica 19: 304
    PubMedSearch in Google Scholar
  • 8 Hougie C. 1962; A simple assay method for Factor X. Proceedings of the Societies for Experimental Biology and Medicine 109: 754
    PubMedSearch in Google Scholar
  • 9 Jackson CM, Johnson TF, Hanahan DJ. 1968; Studies on bovine Factor X. I. Large-scale purification of the bovine plasma protein possessing Factor X activity. Biochemistry 7: 4492
    CrossrefPubMedSearch in Google Scholar
  • 10 Jesty J, Spencer AK, Nemerson Y. 1974; The mechanisms of activation of Factor X. Journal of Biological Chemistry 249: 5614
    PubMedSearch in Google Scholar
  • 11 Loeliger EA, Van Der Esch B, Mattern MJ, Henker HC. 1964; The biological disappearance rate of prothrombin, Factors VII, IX and X from plasma in hypothyroidism, hyperthyroidism, and during fever. Thrombosis et Diathesis Haemorrhagica 19: 267
    PubMedSearch in Google Scholar
  • 12 McFarlane AS. 1958; Efficient tracer labelling of proteins with iodine Nature (London). 182: 53
    PubMedSearch in Google Scholar
  • 13 Reeve EB, Roberts JE. 1959; The kinetics of the distribution and breakdown of I-131- albumin in the rabbit. Journal of General Physiology 43: 415
    CrossrefPubMedSearch in Google Scholar
  • 14 Rosenberg JS, Beeler DL, Rosenberg RD. 1975; Activation of human prothrombin by highly purified human Factors V and Xa in the presence of human antithrombin. Journal of Biological Chemistry 250: 1607
    PubMedSearch in Google Scholar
  • 15 Solomon AK. 1953; The kinetics of biological processes. Special problems connected with the use of tracers Advances in Biology, Medicine and Physics 3: 65
    PubMedSearch in Google Scholar
  • 16 Stenflo J. 1977; Vitamin K, prothrombin and gamma-carboxyglutamic acid. New England Journal of Medicine 296: 624
    CrossrefPubMedSearch in Google Scholar
  • 17 Takeda Y. 1964; Metabolism and distribution of autologous and homologous albumin-I-131 in the dog. American Journal of Physiology 206: 1223
    PubMedSearch in Google Scholar
  • 18 Takeuchi T, Takeda Y. 1978 Physicochemical and biological properties of canine prothrombin and thrombin. Thrombosis Research. (in press)
    PubMedSearch in Google Scholar
  • 19 Van Oosterom AT, Mattie H, Hermens WT, Veltakamp JJ. 1976; The influence of the thyroid function on the metabolic rate of prothrombin, Factor VII, and Factor X in the rats. Thrombosis and Haemostasis 35: 607
    Thieme ConnectPubMedSearch in Google Scholar