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Thromb Haemost 1978; 40(02): 272-287
DOI: 10.1055/s-0038-1648662
Original Article
Schattauer GmbH Stuttgart

Thromboplastin Calibration

Experience of the Dutch Reference Laboratory for Anticoagulant Control
E A Loeliger
The Leiden University Medical Centre, Department of Haematology, Leiden, The Netherlands
,
R. van der Hoeff-van Halem
The Leiden University Medical Centre, Department of Haematology, Leiden, The Netherlands
,
L P van Halem-Visser
The Leiden University Medical Centre, Department of Haematology, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 07 September 1977

Accepted 08 January 1978

Publication Date:
12 July 2018 (online)

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Summary

Standardization of the thromboplastin most commonly used for anticoagulant control in The Netherlands has been achieved by the Dutch Reference Laboratory for Anticoagulant Control. The system established for control indicates the performance characteristics of each batch of thromboplastin in patients relative to a national calibration batch. Calibration constants were assessed according to an ICTH/ICSH proposal and with a reproducibility of approximately 2% (CV). With the use of a given calibration constant of the International Reference Preparation of Thromboplastin (1.0) and after assessment of the calibration constant of the National Reference Preparation in international terms (0.93), the accuracy of calibration with the various modifications of the calibration procedure is of the order of magnitude of 3% (CV). On the basis of the calibration protocol, physicians will be provided with correlation tables containing, for their convenience, prothrombin (thromboplastin) times in terms of the reference thromboplastin (uniform, i. e., batch-independent, prothrombin times), conventional prothrombin activity (percentages), and in due course with International Calibrated Ratios.* With this approach current practice in the prescription of oral anticoagulants will be maintained. Opportunity is given, however, to aim at internationally proposed therapeutically optimal target values.

 

  • References

  • 1 Averdunk R, Borner K. 1974; Standardisierung des Quick-Testes Referenz-Thromboplastin oder Referenz-Plasmen. Blut 28: 445
    CrossrefPubMedSearch in Google Scholar
  • 2 Bailey EL, Harper TA, Pinkerton PH. 1971; The “therapeutic range” of the one-stage prothrombin time in the control of anticoagulant therapy. The effect of different thromboplastin preparations Canadian Medical Association Journal 105: 1041
    PubMedSearch in Google Scholar
  • 3 Bangham DR, Biggs R, Brozovic M, Denson KW E. 1973; Calibration of five different thromboplastins, using fresh and freeze-dried plasma. Thrombosis et Diathesis Haemorrhagica 29: 228
    PubMedSearch in Google Scholar
  • 4 Bangham DR, Loeliger EA. 1977 Stability of the WHO international reference preparation (former ICTH reference thromboplastin 67/40). Thrombosis and Haemostasis, to be published.
    PubMedSearch in Google Scholar
  • 5 Biggs R, Denson KW E. 1967; Standardization of the one-stage prothrombin time for the control of anticoagulant therapy. British Medical Journal 1: 84
    CrossrefPubMedSearch in Google Scholar
  • 6 Biggs R, Bangham DR. 1971; Standardization of the one-stage prothrombin time test for the control of anticoagulant therapy. Availability and use of thromboplastin reference preparations British Medical Journal 3: 470
    CrossrefPubMedSearch in Google Scholar
  • 7 Boekhout-Mussert MJ, Kolk-Schaap PJ, Van Der Hermans J, Loeliger EA. 1978 Comparative study of the clinical validity of the three types of thromboplastin (human, ox, and rabbit). To be published
    PubMedSearch in Google Scholar
  • 8 Bradlow BA, Saunders C, Whitbread P, Lurie A, Penfold GK, Metz J. 1974; Standardisation of the laboratory control of anticoagulant therapy. South African Medical Journal 48: 1857
    PubMedSearch in Google Scholar
  • 9 Brozovic M, Gurd LJ, Robertson I, Bangham DR. 1971; Stability of prothrombin and factor VII in freeze-dried plasma. Journal of Clinical Pathology 24: 690
    CrossrefPubMedSearch in Google Scholar
  • 10 Brozovic M, Howarth DJ, Halem-Visser LP, Van. Loeliger EA. 1973; Stability of freeze-dried plasma prepared from patients on oral anticoagulants. Journal of Clinical Pathology 26: 857
    CrossrefPubMedSearch in Google Scholar
  • 11 Denson KW E. 1971; International and national standardization of control of anticoagulant therapy in patients receiving coumarin and indanedione drugs using calibrated thromboplastin preparations. Journal of Clinical Pathology 24: 460
    CrossrefPubMedSearch in Google Scholar
  • 12 Duk-Wierda CA, Van Hermans J, Loeliger EA, Roos J. 1977; Interlaboratory oral anticoagulant quality assessment by the Netherlands Federation of Thrombosis Services. Thrombosis and Haemostasis 37: 509
    Thieme ConnectPubMedSearch in Google Scholar
  • 13 Grimmer Ø. 1977 Personal communication.
    PubMedSearch in Google Scholar
  • 14 Ingram GI C, Hills M. 1976; Reference method for the one-stage prothrombin time test on human blood. Thrombosis and Haemostasis 36: 231
    PubMedSearch in Google Scholar
  • 15 Ingram GI C, Hills M. 1978 Second draft report of the ICSH/ICTH Expert Panel on Oral Anticoagulant Control, presented at the occasion of the 22nd Annual Meeting of the International Committee on Thrombosis and Haemostasis, Kyoto 1976. To be published
    PubMedSearch in Google Scholar
  • 16 Kahan J, Noren I. 1975; Use of a lyophilized reference plasma to compare coagulation test procedures. Thrombosis et Diathesis Haemorrhagica 34: 426
    PubMedSearch in Google Scholar
  • 17 Loeliger EA. 1972; Progress in the control of oral anticoagulation. Thrombosis et Diathesis Haemorrhagica 28: 109
    PubMedSearch in Google Scholar
  • 18 Loeliger EA. 1974; Performance characteristics of reference thromboplastins. American Journal of Clinical Pathology 62: 574
    PubMedSearch in Google Scholar
  • 19 Loeliger EA, Halem-Visser LP. 1975; A simplified thromboplastin calibration procedure for standardization of anticoagulant control. Thrombosis et Diathesis Haemorrhagica 33: 172
    PubMedSearch in Google Scholar
  • 20 Loeliger EA, Duk-Wierda CA, Van. Roos J. 1977. Quality control and standardization of the prothrombin time. Second International Symposion on Quality Control Tokyo: 1976
    Search in Google Scholar
  • 21 Loeliger EA, Duk-Wierda CA, Van. Roos J. 1977; Qualitàtskontrolle und Standardisierung der Prothrombinzeitbestimmung zur Kontrolle oraler Antikoagulation. Blut 34: 147
    CrossrefPubMedSearch in Google Scholar
  • 22 Loeliger EA. 1977; Stability studies on lyophilized reference thromboplastins for standardization of prothrombin times. Lancet 2: 1279
    PubMedSearch in Google Scholar
  • 23 Miale JB, Kent JW. 1972; Standardization of the therapeutic range for oral anticoagulants based on standard reference plasmas. American Journal of Clinical Pathology 57: 80
    CrossrefPubMedSearch in Google Scholar
  • 24 Owren PA. 1959; Thrombotest. A new method for controlling anticoagulant therapy Lancet 2: 3
    PubMedSearch in Google Scholar
  • 25 Patterson BB, Puls JL. 1976; Standardization of prothrombin and activated partial thromboplastin time reagents and controls. American Journal of Clinical Pathology 65: 213
    CrossrefPubMedSearch in Google Scholar
  • 26 Poller L. 1967; A national standard for anticoagulant therapy. Lancet 1: 491
    PubMedSearch in Google Scholar
  • 27 Poller L. 1975; The British system for anticoagulant control. Thrombosis et Diathesis Haemorrhagica 33: 157
    PubMedSearch in Google Scholar
  • 28 Thomson JM, Chart IS. 1970; The system for laboratory control of oral anticoagulant therapy using the British Comparative Thromboplastin. Journal of Medical Laboratory Technology 27: 207
    PubMedSearch in Google Scholar
  • 29 Zucker S, Cathey MH, Sox PJ, Hall EC. 1969; Standardization of laboratory tests for controlling anticoagulant therapy. American Journal of Clinical Pathology 53: 348
    PubMedSearch in Google Scholar