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Thromb Haemost 1994; 72(01): 044-053
DOI: 10.1055/s-0038-1648809
Original Article
Schattauer GmbH Stuttgart

Plasminogen Activator Inhibitor-1 Expression in Human Liver and Healthy or Atherosclerotic Vessel Walls

N Chomiki
The Laboratory of Hematology, CHU Timone, Marseille, France
,
M Henry
The Laboratory of Hematology, CHU Timone, Marseille, France
,
M C Alessi
The Laboratory of Hematology, CHU Timone, Marseille, France
,
F Anfosso
The Laboratory of Hematology, CHU Timone, Marseille, France
,
I Juhan-Vague
The Laboratory of Hematology, CHU Timone, Marseille, France
› Institutsangaben
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Publikationsverlauf

Received 14. September 1993

Accepted after resubmission 22. März 1994

Publikationsdatum:
12. Juli 2018 (online)

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Summary

Individuals with elevated levels of plasminogen activator inhibitor type 1 are at risk of developing atherosclerosis. The mechanisms leading to increased plasma PAI-1 concentrations are not well understood. The link observed between increased PAI-1 levels and insulin resistance has lead workers to investigate the effects of insulin or triglyceride rich lipoproteins on PAI-1 production by cultured hepatocytes or endothelial cells. However, little is known about the contribution of these cells to PAI-1 production in vivo. We have studied the expression of PAI-1 in human liver sections as well as in vessel walls from different territories, by immunocytochemistry and in situ hybridization.

We have observed that normal liver endothelial cells expressed PAI-1 while parenchymal cells did not. However, this fact does not refute the role of parenchymal liver cells in pathological states.

In healthy vessels, PAI-1 mRNA and protein were detected primarily at the endothelium from the lumen as well as from the vasa vasorum. In normal arteries, smooth muscle cells were able to produce PAI-1 depending on the territory tested. In deeply altered vessels, PAI-1 expression was observed in neovessels scattering the lesions, in some intimal cells and in smooth muscle cells. Local increase PAI-1 mRNA described in atherosclerotic lesions could be due to the abundant neovascularization present in the lesion as well as a raised expression in smooth muscle cells. The increased PAI-1 in atherosclerosis could lead to fibrin deposit during plaque rupture contributing further to the development and progression of the lesion.

 

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