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Thromb Haemost 1994; 72(02): 309-312
DOI: 10.1055/s-0038-1648858
Original Article
Schattauer GmbH Stuttgart

The Calcium Modulator Nifedipine Exerts Its Antiaggregatory Property via a Nitric Oxide Mediated Process

R Berkels
The Institut für Pharmakologie, Universität zu Köln Germany
,
W Klaus
The Institut für Pharmakologie, Universität zu Köln Germany
,
M Boiler
The Institut für Pharmakologie, Universität zu Köln Germany
,
R Rösen
The Institut für Pharmakologie, Universität zu Köln Germany
› Author Affiliations
Further Information

Publication History

Received 02 19 1993

Accepted after resubmission 25 April 1994

Publication Date:
24 July 2018 (online)

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Summary

The in vitro effect of nifedipine, a calcium channel blocker of the dihydropyridine (DHP) type, on platelet aggregation was reinvestigated considering especially the capability of platelets to form endogenous nitric oxide (NO). We studied the dose-dependent antiaggregatory property of nifedipine in porcine platelet rich plasma. Aggregation was stimulated by collagen (7.5 ¼g/ml). Nifedipine inhibited collagen-induced platelet aggregation with an IC50 of 380 nmol/1. The antiaggregatory effect of nifedipine could be significantly diminished by N-nitro-L-arginine (NNA) in a concentration dependent manner, whereas oxy haemoglobin (4 ¼M), a NO scavenger, totally abolished the effect of nifedipine. L-Arginine, the precursor of NO, dose-dependently inhibited the collagen-induced platelet aggregation but did not potentiate the effects of nifedipine. Therefore, we propose that in platelet rich plasma the nifedipine induced inhibition of platelet aggregation is mediated by NO, a potent endogenous inhibitor of aggregation. We could confirm this hypothesis by measuring NO directly with a specific electrode.

 

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