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Thromb Haemost 1994; 72(04): 503-507
DOI: 10.1055/s-0038-1648903
Original Article
Schattauer GmbH Stuttgart

Serological and Virological Markers of Human Parvovirus B19 Infection in Sera of Hemophiliacs

A Große-Bley
1   The institute of Medical Microbiology and Immunology, Munich, Germany
,
A M Eis-Hübinger
1   The institute of Medical Microbiology and Immunology, Munich, Germany
,
R Kaiser
1   The institute of Medical Microbiology and Immunology, Munich, Germany
,
J Oldenburg
2   The institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
,
H H Brackmann
2   The institute of Experimental Hematology and Transfusion Medicine, University of Bonn, Bonn, Germany
,
T F Schwarz
3   The Max v. Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Munich, Germany
,
K E Schneweis
1   The institute of Medical Microbiology and Immunology, Munich, Germany
› Author Affiliations
Further Information

Publication History

Received: 17 December 1993

Accepted after resubmission02 June 1994

Publication Date:
06 July 2018 (online)

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Summary

It is known that parvovirus B19 (B19) is transmitted to hemophiliacs by clotting factors prepared from human plasma. However, it is not clear whether B19 is also transmitted by the more recently used inactivated clotting factor preparations. Therefore, we investigated 69 hemophiliacs, mostly children, receiving only virus-inactivated clotting factors. 49 of them (71%) were B19 IgG-positive and 18 of the IgG-positive hemophiliacs (37%) were also B19 IgM-positive. In contrast, out of 73 age-matched controls only 10 (14%) were IgG-positive, two of them being also IgM-positive. In hemophiliacs treated before 1984 with noninactivated clotting factors, seroprevalence was very similar: 94/136 (69%) presented B19 IgG antibodies as compared to their age-matched controls with 16/50 (32%). Out of the 94 IgG-positive patients 24 (26%) were IgM-positive, whereas IgM antibodies were never found in 16 sera of 16 IgG-positive controls. In 4 out of 24 IgM positive hemophiliacs, B19 DNA was detected in the sera by using the polymerase chain reaction. However, B19 DNA was also found in 3/69 anti-B19 IgM-negative, HIV-infected hemophiliacs (all three patients in CDC stage IV). Since it seems unlikely that the results only represent passive acquisition of B19 DNA from blood products and induction of antibodies by immunization with inactivated antigen, the observations rather suggest that infection with B19 is transmitted by clotting factors, including those treated for virus inactivation.

 

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