Expression of Platelet Membrane Glycoprotein V in Human Megakaryocytes and Megakaryocytic Cell Lines: A Study Using a Novel Monoclonal Antibody against GPV

Toshiro Takafuta; Kingo Fujirmura; Hironori Kawano; Masaaki Noda; Tetsuro Fujimoto; Kenji Oda; Takeshi Shimomura; Atsushi Kuramoto

doi:10.1055/s-0038-1648955

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1994; 72(05): 762-769
DOI: 10.1055/s-0038-1648955

Original Article

Schattauer GmbH Stuttgart

Expression of Platelet Membrane Glycoprotein V in Human Megakaryocytes and Megakaryocytic Cell Lines: A Study Using a Novel Monoclonal Antibody against GPV

Toshiro Takafuta

The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

,

Kingo Fujirmura

The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

,

Hironori Kawano

The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

,

Masaaki Noda

The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

,

Tetsuro Fujimoto

The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

,

Kenji Oda

The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

,

Takeshi Shimomura

The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

,

Atsushi Kuramoto

The Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima, Japan

› Author Affiliations

Abstract

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Summary

Glycoprotein V (GPV) is a platelet membrane protein with a molecular weight of 82 kD, and one of the leucine rich glycoproteins (LRG). By reverse transcription-polymerase chain reaction (RT-PCR), GPV cDNA was amplified from mRNA of platelets and megakaryocytic cell lines. However, since there are few reports indicating whether GPV protein is expressed in megakaryocytes as a lineage and maturation specific protein, we studied the GPV expression at the protein level by using a novel monoclonal antibody (1D9) recognizing GPV. Flow cytometric and immunohistochemical analysis indicated that GPV was detected on the surface and in the cytoplasm of only the megakaryocytes in bone marrow aspirates. In a megakaryocytic cell line UT-7, GPV antigen increased after treatment with phorbol-12-myri-state-13-acetate (PMA). These data indicate that only megakaryocytes specifically express the GPV protein among hematopoietic cells and that the expression of GPV increases with differentiation of the megakaryocyte as GPIb-IX complex.

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References

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